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2016, vol. 73, br. 5, str. 449-457
Udruženost ekspresije vaskularnog endotelnog faktora rasta sa patohistološkim parametrima kožnih melanoma
aVojnomedicinska akademija, Klinika za plastičnu hirurgiju i opekotine, Beograd + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd
bVojnomedicinska akademija, Institut za patologiju i sudsku medicinu, Beograd
cUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd + Vojnomedicinska akademija, Institut za patologiju i sudsku medicinu, Beograd
dUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd + Vojnomedicinska akademija, Institut za medicinska istraživanja, Beograd
eDorset County Hospital Foundation Trust, Dorchester, UK
fUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd + Vojnomedicinska akademija, Odsek za prevenciju i kontrolu bolničkih infekcija, Beograd
gUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd + Vojnomedicinska akademija, Klinika za kožne i polne bolesti, Beograd

e-adresamilomir.gacevic@gmail.com
Sažetak
Uvod/Cilj. Melanom je najagresivniji maligni tumor kože. Do sada su objavljeni kontradiktorni podaci o vaskularnom endotelnom faktoru rasta (VGEF) prisutnom u uzorcima tumora, kao i njegovoj ulozi u progresiji i prognozi melanoma kože. Cilj ove studije bio je da se ispita značaj ekspresije VEGF kao prognostičkog parametra kod melanoma. Metode. Eksperimentalna grupa sastojala se od 81 bolesnika sa primarnim melanomom kože lečenih na Vojnomedicinskoj akademiji, Beograd, od 2009. do 2013. godine. Kontrolna grupa sastojala se od 20 bolesnika sa displastičnim i 20 sa benignim nevusima. Stratifikacija je izvršena prema polu, starosti, kliničkom i histološkom stadijumu, lokalizaciji, histološkom tipu, Clark-u, Breslovu, broju mitoza, regresiji i ulceraciji, prisustvu tumorinfiltrišucih limfocita i načinu širenja. Imunohistohemijsko bojenje izvedeno je na biopsijama kože uz upotrebu DAKO anti-VEGF antitela, LSABTM + HRP, Liquid DAB i mikrotalasnog demaskiranja u DAKO pH 9.0 rastvoru. Za statističku obradu korišćenisu testovi ANOVA, Bonferroni post-test, Mann-Whitney i Wilcoxon test. Rezultati. Prosečan intenzitet VEGF bojenja bio je značajno veći u melanomima nego u benignim ili displastičnin nevusima, a u melanomima bio je najveći u Ia i IV kliničkom stadijumu. Većina melanoma u pT1a patološkom stadijumu imala je najveće prosečne vrednosti VEGF. Melanomi sa najvećim brojem mitoza (> 6) imali su značajno veći intenzitet VEGF bojenja nego oni sa < 2 mitoze. Melanomi bez limfocitne infiltracije imali su najveće vrednosti VEGF, dok su oni sa najintenzivnijom infiltracijom imali najniže vrednosti. Ova razlika je bila statistički visokoznačajna. Melanomi sa limfnim i hematogenim načinom širenja imali su najveće prosečne vrednosti VEGF. Nije bilo statistički značajne korelacije između intenziteta VEGF bojenja i bilo kog drugog parametra. Zaključak. U primarnim melanomima kože povećana ekspresija VEGF značajna je za tumorsku progresiju, a povezana je kako sa odsustvom limfocitne infiltracije, tako i sa prisustvom limfovaskularne invazije. Potrebna su detaljnija prospektivna istraživanja ekspresije VEGF kao prognostičkog parametra kod melanoma na većem broju bolesnika.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/vsp140804027G
objavljen u SCIndeksu: 06.05.2016.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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