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Materia medica
2018, vol. 34, br. 3, str. 1649-1655
jezik rada: srpski
vrsta rada: originalan članak
doi:10.5937/MatMed1803649J


Endokrine ćelije pankreasa u pacova hronično tretiranih kadfmijumom
aUniverzitet u Nišu, Medicinski fakultet
bKlinički Centar Crne Gore, Centar za abdominalnu hirurgiju, Podgorica, Crna Gora
cKlinički Centar Crne Gore, Klinika za Gastroenterologiju i Hepatologiju, Podgorica,Crna Gora

e-adresa: ninajancic@live.com

Sažetak

Kadmijum (Cd) je mekan srebtrnasto-beli metal, jedan od 126 prioritetnih zagađivača, a svrstan je i u grupu humanih karcinogena I kategorije.Cilj rada je mikromorfološko i funkcionalno ispitivanje endokrinog pankreasa pacova hronično tretiranih kadmijumom. Za istraživanje su korišćeni beli Wistar pacovi ženskog pola, starosti 35-37 dana, težine 120-140 g.Ukupno je bilo 22 životinje koje su podeljene na kontrolnu (n=11) i eksperimentalnu grupu (n=11). Eksperimentalna grupa je svakodnevno tretirana sa 15mg/kg CdCl2 rastvorenog u pijaćoj vodi. Kontrolna grupa nije bila podvrgnuta nikakvom tretmanu. Svi pacovi su čuvani u kontrolisanim laboratorijskim uslovima. Posle tri meseca, sve životinje su žrtvovane. Tkivo pankreasa je rutinski obrađeno i kalupljeno u parafi n. Na 4μm presecima su primenjene HE i imunohistohemijska ABC metoda sa antitelima na: chromogranin A, insulin,glucagon,somatostatin, pankreasni polipeptid, i peptid YY. U životinja eksperimentalne grupe su nađene guste, hiperplastične B ćelije koje zaposedaju skoro čitavu površinu insule. Prisutna je i hiperplazija A ćelija sa izraženom funkcionalnom aktivnošću. Osim po obodu hiperplastičnih insula, pojedinačne A ćelije se nalaze i u acinusima u kojima je njihova aktivnost znatno povećana. Zapažen je povećan mitotski indeks i odsustvo citoplazmatskih produžetka D ćelija. Izražena je hiperplazija PP ćelija, sa znacima kako morfološkog tako i funkcionalnog polimorfi zma. Prisustvo PP ćelija je evidentirano i u hiperplastičnom i displastičnom epitelu većih duktusa. Samo u životinja eksperimentalne grupe smo našli ćelije koje sekretuju peptid YY. Ove ćelije imaju identičnu topografi ju kao i A ćelije, ali je njihov broj znatno manji. Hronično izlaganje kadmijumu remeti strukturu i funkciju endokrinog pankreasa.Sve pankreasne endokrine ćelije su pogođene.

Ključne reči

endokrine ćelije pankreasa; pacovi; kadmijum; trovanje; imunohistohemija

Reference

*** (2011) National Toxicology Program(NTP): 12th report on carcinogens. NC: National Institution of Environmental Health Sciences, U.S. Department of Health and Human Services, Public Health Service, 80-82
Abnosi, M.H., Golami, S. (2017) Cadmium chloride treatment of rats significantly impairs membrane integrity of mesenchymal stem cells via electrolyte imbalance and lipid peroxidation, a possible explanation of Cd related osteoporosis. Iran J Basic Med Sci, 20(3), 280-287
Alán, L., Olejár, T., Cahová, M., Zelenka, J., Berková, Z., Smětáková, M., Saudek, F., Matěj, R., Ježek, P. (2015) Delta cell hyperplasia in adult Goto-Kakizaki (GK/MolTac) diabetic rats. Journal of Diabetes Research, 2015, 385-395
Alosi, J.A., McFadden, D.W. (2009) Peptide YY mediates inhibition of tumor growth and inflammation. Methods Mol Biol, 512, 377-394
Andujar, P., Bensefa-Colas, L., Descatha, A. (2010) Acute and chronic cadmium poisoning. Rev Med Intern, 31, 107-115
Cannino, G., Ferruggia, E., Luparello, C., Rinaldi, A.M. (2009) Cadmium and mitochondria. Mitochondrion, 9(6), 377-384
Cartularo, L., Laulicht, F., Sun, H., Kluz, T., Freedman, J.H., Costa, M. (2015) Gene expression and pathway analysis of human hepatocellular carcinoma cells treated with cadmium. Toxicology and Applied Pharmacology, 288(3), 399-408
Cuypers, A., Plusquin, M., Remans, T., Jozefczak, M., Keunen, E., Gielen, H., Opdenakker, K., Nair, A.R., Munters, E., Artois, T.J., Nawrot, T., Vangronsveld, J., Smeets, K. (2010) Cadmium stress: An oxidative challenge. BioMetals, 23(5), 927-940
Dover, N.E., Patel, N.Y., Stýblo, M. (2018) Impact of in vitro heavy metal exposure on pancreatic β-cell function. Toxicology Letters, 299, 137-144
Gepts, W., de Mey, J. (1978) Pancreatic polypeptide. Diabete Metab, Dec; 4(4), 275-283
Govil, N., Chaudhary, S., Waseem, M., Parvez, S. (2012) Postnuclear supernatant: An in vitro model for assessing cadmium-induced neurotoxicity. Biological Trace Element Research, 146(3), 402-409
Hogervorst, J., Plusquin, M., Vangronsveld, J., Nawrot, T., Cuypers, A., van Hecke, E., Roels, H.A., Carleer, R., Staessen, J.A. (2007) House dust as possible route of environmental exposure to cadmium and lead in the adult general population. Environmental Research, 103(1), 30-37
Holemans, K., Aerts, L., van Assche, F.A. (2003) Lifetime consequences of abnormal fetal pancreatic development. Journal of Physiology, 547(1), 11-20
Huang, C.L., Sun, L., Moonga, B.S., Zaidi, M. (2006) Molecular physiology and pharmacology of calcitonin. Cell Mol Biol (Noisy-le-grand), 52(3), 33-43
International Agency For Research On Cancer Iarc (2012) Monographs on the evaluation of carcinogenic risks to humans. Cadmium and Cadmium Compounds, 100C, 121-145
Järup, L., Åkesson, A. (2009) Current status of cadmium as an environmental health problem. Toxicology and Applied Pharmacology, 238(3), 201-208
Kang, H., Kim, S., Lim, T.S., Lee, H.W., Choi, H., Kang, C.M., Kim, H.G., Bang, S. (2014) A case of Alpha-cell nesidioblastosis and hyperplasia with multiple glucagon-producing endocrine cell tumor of the pancreas. Korean Journal of Gastroenterology, 63(4), 253-257
Kawahara, H., Takashima, Y., Nakamura, M., Yamagami, A. (1975) Electron microscopic study of the cytotoxicity of cadmium and mercury in vitro. Journal of Dental Research, 54(1), 125-130
Kiessl, G.R.R., Laessle, R.G. (2016) Stress inhibits PYY secretion in obese and normal weight women. Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity, 21(2), 245-249
Klaassen, C.D., Liu, J., Diwan, B.A. (2009) Metallothionein protection of cadmium toxicity. Toxicology and Applied Pharmacology, 238(3), 215-220
Lima, C.A.I., Pestana, I.A., Azevedo, L.S., Ribeiro, D.P., Almeida, M.G., Prins, C.L., Marciano, C.R., Souza, C.M.M. (2019) Bioconcentration and translocation of Cd and Hg in a tomato (Solanum lycopersicum) from cultivated soils in southeastern Brazil. Environmental Monitoring and Assessment, 191(2), 103-103
Liu, T., He, W., Yan, C., Qi, Y., Zhang, Y. (2011) Roles of reactive oxygen species and mitochondria in cadmium-induced injury of liver cells. Toxicology and Industrial Health, 27(3), 249-256
Marcano, L.B.C., Carruyo, I.M., Montiel, X.M., Morales, C.B., de Soto, P.M. (2009) Effect of cadmium on cellular viability in two species of microalgae (Scenedesmus sp. and Dunaliella viridis). Biological Trace Element Research, 130(1), 86-93
Martelli, A., Rousselet, E., Dycke, C., Bouron, A., Moulis, J.M. (2006) Cadmium toxicity in animal cells by interference with essential metals. Biochimie, 88(11), 1807-1814
Nordberg, G.F., Nogawa, K., Nordberg, M.F., Friberg, L.T. (2007) Cadmium. u: Nordberg G.F., Fowler B.A., Nordberg M., Friberg L. [ur.] Handbook on the Toxicology of Metals, Amsterdam: Elsevier, 445-486
Olsson, I.M., Bensryd, I., Lundh, T., Ottosson, H., Skerfving, S., Oskarsson, A. (2002) Cadmium in blood and urine-impact of sex, age, dietary intake, iron status, and former smoking-association of renal effects. Environmental Health Perspectives, 110(12), 1185-1190
Peng, L., Huang, Y.T., Zhang, F., Chen, J.Y., Huo, X. (2018) Chronic cadmium exposure aggravates malignant phenotypes of nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway via hypermethylation of the casein kinase 1α promoter. Cancer Management and Research, Volume 11, 81-93
Petering, D.H., Krezoski, S., Tabatabai, N.M. (2009) Metallothionein toxicology: metal ion trafficking and cellular protection. Met Ions Life Sci, 5, 353-397
Plagemann, A., Heidrich, I., Rohde, W., Gotz, F., Dörner, G. (1992) Hyperinsulism during differentiation of the hypothalamus is a diabetogenic and obesity risk factor in rats. Neuroendocrinology, Letters, 5, 373-378
Richter, P., Faroon, O., Pappas, R. (2017) Cadmium and Cadmium/Zinc Ratios and tobacco-related morbidities. International Journal of Environmental Research and Public Health, 14(10), 1154-1154
Sacchi, T., Bani, D., Biliotti, G. (1985) Nesidioblastosis and islet cell changes related to endogenous hypergastrinemia. Virchows Archiv B Cell Pathology Including Molecular Pathology, 48(3), 261-276
Schwartz, T.W. (1979) Pancreatic-polypeptide (PP) and endocrine tumours of the pancreas. Scand J Gastroenterol Suppl, 53, 93-100
Turner, A. (2019) Cadmium pigments in consumer products and their health risks. Science of The Total Environment, 657, 1409-1418
Vahter, M., Åkesson, A., Lidén, C., Ceccatelli, S., Berglund, M. (2007) Gender differences in the disposition and toxicity of metals. Environmental Research, 104(1), 85-95
Ware, G. (2007) Reviews of environmental contamination and toxicology. London: Springer, 48-55
Zhang, C., Yuan, X., Mao, W., Yue, L., Kong, X., Gao, Y., Luo, L., Yin, Z. (2010) Inhibition of cadmium-induced apoptosis by glutathione S-transferase P1 via mitogen-activated protein kinases and mitochondrial pathways. Environmental Toxicology and Pharmacology, 30(2), 202-208
Zhou, T., Jia, X., Chapin, R.E., Maronpot, R.R., Harris, M.W., Liu, J., Waalkes, M.P., Eddy, E.M. (2004) Cadmium at a non-toxic dose alters gene expression in mouse testes. Toxicology Letters, 154(3), 191-200