Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:[2]
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:6
  • preuzimanja u poslednjih 30 dana:1

Sadržaj

članak: 3 od 4  
Back povratak na rezultate
2015, vol. 34, br. 3, str. 338-343
Ekspresija HMGA2 u karcinomu bubrega i njen klinički značaj
The Zhongshan Affiliated Hospital of Dalian University, Department of Urology, Dalian, China

e-adresaqizhongfucn@163.com
Ključne reči: karcinom bubrega; visokopokretljiva grupa A; imunohistohemija; RT-PCR
Sažetak
Uvod: Cilj studije je da se otkrije ekspresija HMGA2 u karcinomu bubrega kako bi se istražio njen odnos sa kliničkom patologijom kao i značaj u prognozi. Metode: Ekspresije mDNK i proteina HMGA2 određene su u 50 uzoraka karcinoma bubrega, 50 odgovarajućih uzoraka okolnog normalnog tkiva bubrega i 40 uzoraka benignih tumora bubrega putem reverzne transkripcije - lančane reakcije polimeraze i imunohistohemijskim testovima. Analiza ekspresije obavljena je zajedno sa analizom kliničkih podataka. Rezultati: Relativni nivoi ekspresije mDNK HMGA2 u karcinomu bubrega, tkivima benignih bubrežnih tumora i tkivima okolnog normalnog bubrežnog tkiva bili su redom: 0,84± 0,23, 0,19± 0,06 i 0,08± 0,04. Pozitivne stope proteina HMGA2 bile su 68,0%, 7,5% i 2,0%, uz postojanje značajne razlike (P<0,05). Ekspresija HMGA2 nije značajno korelisala sa polom, starošću, veličinom tumora i histološkim tipom (P> 0,05), ali je bila u značajnoj korelaciji sa stupnjevima TNM i metastazama limfnih cvorova (P<0,05). ZakJjučak: Ekspresije gena i proteina HMGA2 u karcinomu bubrega bile su u bliskoj korelaciji sa formiranjem, progresijom i metastazama tumora. HMGA2 bi mogao da postane moćan novi patološki marker i prognostički faktor za karcinom bubrega.
Reference
Belge, G., Meyer, A., Klemke, M., Burchardt, K., Stern, C., Wosniok, W., Loeschke, S., Bullerdiek, J. (2008) Upregulation ofHMGA2 in thyroid carcinomas: A novel molecular marker to distinguish between benign and malignant follicular neoplasias. Genes, Chromosomes and Cancer, 47(1): 56-63
Borrmann, L., Schwanbeck, R., Heyduk, T., Seebeck, B., Rogalla, P., Bullerdiek, J., et al. (2003) High mobility group A2 protein and its derivatives bind a specific region of the promoter of DNA repair gene ERCC1 and modulate its activity. Nucleic Acids Research, 31(23): 6841-6851
Cohen, H.T., McGovern, F.J. (2005) Renal-Cell Carcinoma. New England Journal of Medicine, 353(23): 2477-2490
de Martino, I., Visone, R., Wierinckx, A., Palmieri, D., Ferraro, A., Cappabianca, P., Chiappetta, G., Forzati, F., Lombardi, G., Colao, A., Trouillas, J., Fedele, M., Fusco, A. (2009) HMGA Proteins Up-regulate CCNB2 Gene in Mouse and Human Pituitary Adenomas. Cancer Research, 69(5): 1844-1850
Fehr, A., Meyer, A., Heidorn, K., Röser, K., Löning, T., Bullerdiek, J. (2009) A link between the expression of the stem cell marker HMGh2, grading, and the fusion CRTCl-MAML2 in mucoepidermoid carcinoma. Genes, Chromosomes and Cancer, 48(9): 777-785
Franciso, H.D., Kim, H.L., Seligson, D., Liu, X. (2004) Using protein expressions to predict survival in clear cell renal carcinoma. Clinical Cancer Research, 10: 5464-71
Hammond, S.M., Sharpless, N.E. (2008) HMGA2, MicroRNAs, and Stem Cell Aging. Cell, 135(6): 1013-1016
Huang, M., Chen, C.C., Chang, L.C. (2009) Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer. International Journal of Colorectal Disease, 24(11): 1281-1286
Li, A.Y.J., Boo, L.M., Wang, S.Y., Lin, H.H., Wang, C.C.C., Yen, Y., Chen, B.P.C., Chen, D.J., Ann, D.K. (2009) Suppression of Nonhomologous End Joining Repair by Overexpression of HMGA2. Cancer Research, 69(14): 5699-5706
Mankoff, D.A., Thompson, J.A., Gold, P., Eary, J.F., Guinee, D.G.Jr., Samlowski, W.E. (1997) Identification of interleukin-2-induced complete response in metastatic renal cell carcinoma by FDG PET despite radiographic evidence suggesting persistent tumor. American Journal of Roentgenology, 169(4): 1049-1050
Mayr, C., Hemann, M.T., Bartel, D.P. (2007) Disrupting the pairing between let-7 and HMGA2 enhances oncogenic transformation. Science, 315(5818): 1576-1579
Medeiros, F., Araujo, A.R., Erickson-Johnson, M.R., Kashyap, P.C., Cin, D.I.P., Nucci, M., et al. (2010) HMGA1 and HMGA2 rearrangements in mass-forming endometriosis. Genes Chromosomes Cancer, 49: 630-634
Meyer, B., Loeschke, S., Schultze, A., Weigel, T., Sandkamp, M., Goldmann, T., Vollmer, E., Bullerdiek, J. (2007) HMGA2 overexpression in non-small cell lung cancer. Molecular Carcinogenesis, 46(7): 503-511
Miyazawa, J., Mitoro, A., Kawashiri, S., Chada, K.K., Imai, K. (2004) Expression of Mesenchyme-Specific Gene HMGA2 in Squamous Cell Carcinomas of the Oral Cavity. Cancer Research, 64(6): 2024-2029
Nestorov, J., Matić, G., Elaković, I., Tanić, N. (2013) Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR. Journal of Medical Biochemistry, vol. 32, br. 4, str. 325-338
Nishino, J., Kim, I., Chada, K., Morrison, S.J. (2008) Hmga2 Promotes Neural Stem Cell Self-Renewal in Young but Not Old Mice by Reducing p16Ink4a and p19Arf Expression. Cell, 135(2): 227-239
Reeves, R., Beckerbauer, L. (2001) HMGI/Y proteins: Flexible regulators of transcription and chromatin structure. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1519(1-2): 13-29
Tessari, M.A., Gostissa, M., Altamura, S., Sgarra, R., Rustighi, A., Salvagno, C., Caretti, G., Imbriano, C., Mantovani, R., Del, S.G., Giancotti, V., Manfioletti, G. (2003) Transcriptional Activation of the Cyclin A Gene by the Architectural Transcription Factor HMGA2. Molecular and Cellular Biology, 23(24): 9104-9116
Wang, X., Liu, X., Li, A.Y., Chen, L., Lai, L., Lin, H.H., Hu, S., Yao, L., Peng, J., Loera, S., Xue, L., Zhou, B., Zhou, L., Zheng, S., Chu, P., Zhang, S., Ann, D.K., Yen, Y. (2011) Overexpression of HMGA2 Promotes Metastasis and Impacts Survival of Colorectal Cancers. Clinical Cancer Research, 17(8): 2570-2580
Wu, Y., Zhou, B.P. (2008) New insights of epithelial-mesenchymal transition in cancer metastasis. Acta Biochimica et Biophysica Sinica (Shanghai), 40(7): 643-650
Young, A.R.J., Narita, M. (2007) Oncogenic HMGA2: Short or small?. Genes & Development, 21(9): 1005-1009
Yu, F., Yao, H., Zhu, P., Zhang, X., Pan, Q., Gong, C., et al. (2007) Let-7 regulates self-renewal and tumorigenicity of breast cancer cells. Cell, 131: 1109-23