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Vojnosanitetski pregled
2011, vol. 68, br. 7, str. 567-574
jezik rada: engleski
vrsta rada: originalan članak
doi:10.2298/VSP1107567D
Ekspresija regulatornih proteina i proliferativna aktivnost u vezi sa fenotipskim karakteristikama karcinoma gornjeg urotelijuma
aUniverzitet u Prištini (Kosovska Mitrovica), Medicinski fakultet
bSchool of Medicine, Institute of Pathology, Niš
cSchool of Medicine, Clinic of Surgery, Niš
dUniverzitet u Nišu, Medicinski fakultet
eKlinički centar Niš

e-adresa: dravel@open.telekom.rs

Projekat

Etiologija, dijagnostika, prevencija, i terapija endemske nefropatije i sa njom povezanih tumora urotela - značaj istraživanja genoma i proteoma (MPNTR - 175092)

Sažetak

Uvod/Cilj. Deregulacija normalnog ćelijskog ciklusa je česta kod karcinoma gornjeg urotelijuma (KGU). Cilj ovog rada bio je da se ispita ekspresija regulatornih proteina ćelijskog ciklusa (p53, p16, ciklin D1, HER-2) i proliferativna aktivnost Ki-67 kod KGU i da se utvrdi njihov međusobni uticaj i uticaj na fenotipske karakteristike KGU. Metode. Kod 44 bolesnika sa KGU urađene su patohistološka i imunohistohemijska analiza (p53, p16, cyclin D1, HER-2 i Ki-67) tumora. Rezultati. Prekomerna ekspresija/izmenjena ekspresija p53, p16, ciklina D1 i HER-2 otkrivena je kod 20%, 57%, 64% i 57% tumora, redom. Jedanaest (25%) KGU imalo je visoki proliferativni Ki-67 indeks. Četrdeset bolesnika (91%) imalo je alteraciju najmanje jednog markera, dok su četiri (9%) tumora imala wild-type status. Analiza povezanosti ekspresije molekularnih markera pokazala je da je samo visoka ekspresija p53 bila značajno udružena sa izmenjenom p16 aktivnošću (p < 0,05). Visoki Ki-67 indeks bio je udružen sa visokim stadijumom (p < 0,005), solidnim rastom (p < 0,01), visokim gradusom (p < 0,05) i multifokalnošću (p < 0,05) KGU, dok je visoka ekspresija p53 bila udružena sa solidnim rastom (p < 0,05). U regresionom modelu koji je uključivao sve molekularne markere i fenotipske karakteristike, samo je Ki-67 korelisao sa rastom (p < 0,0001), stadijumom (p < 0,01), gradusom (p < 0,05) i multifokalnošću (p < 0,05) KGU, a ekspresija Ki-67 i HER-2 korelisala je sa limfovaskularnom invazijom (p < 0,05). Zaključak. Ovo istraživanje pokazalo je da su samo negativni regulatorni proteini ćelijskog ciklusa, p53 i p16, bili značajno povezani kod KGU, dok je proliferativni Ki-67 marker bio povezan sa ključnim fenotipskim karakteristikama KGU na najbolji način.

Ključne reči

mokraćna bešika; karcinomi; ćelija, ciklus, proteini; Ki-67 antigen

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