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2012, vol. 36, br. 1, str. 133-139
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Nalaz antitela protiv virusnog arteritisa kod kobila u pripustu
Finding of antibodies against the viral arteritis in mares in the breeding
Univerzitet u Novom Sadu, Poljoprivredni fakultet, Departman za veterinarsku medicinu
Ključne reči: virusni arteritis; konji; SN test
Keywords: viral arteritis; horses; SN test
Sažetak
Virusni arteritis konja (EAV) predstavlja respiratorno, virusno, kontagiozno oboljenje ekvida, a uzročnik je virus iz roda Arterivirus (EVA). Oboljenje karakterišu lezije u malim krvnim sudovima, posebno arteriolama. Oboljenje najčešće protiče subklinički ili akutno. EAV infekcija može izazvati simptome slične gripu odnosno respiratorne simptome bolesti i to kod odraslih konja, abortus kod gravidnih kobila, intersticijalnu pneumoniju kod mladih ždrebadi, i perzistentnu infekciju kod pastuva. Infekcija je moguća samo kod životinja iz porodice ekvida. Prenosi se horizontalno putem aerosola odnosno respiratorne infekcije prijemčivih konja ili prilikom prirodnog odnosno veštačkog osemenjavanja kobila perzistentno zaraženih pastuva. Od seroloških testova razvijeni su serum neutralizacioni test (SN), indirektna imunofluorescencija, agar gel imunodifuzija i ELISA test, a trenutno su najčešće u upotrebi SN test i ELISA (Timoney, 2002.; OIE 2008.). U radu su korišćeni uzorci krvi od sedam kobila, koje se koriste za pripust. Testom serum neuralizacije utvrđeno je da 5 grla imaju pozitivan nalaz što čini 71,43% pozitivnih životinja u ovom zapatu. Grla koja imaju pozitivan titar antitela su pripuštana više puta, sva grla su imala reproduktivnih problema ali je samo kod jednog grla došlo do abortusa. Grla koja imaju negativan nalaz su bila pripuštena prvi put. Kod njih, iako se drže zajedno sa ostalim grlima, nije došlo do horizontalnog prenosa virusa. Pozitivna grla ispoljavaju određene reproduktivne probleme koji se mogu ali ne moraju pripisati ovom virusu.
Abstract
Equine viral arteritis virus (EAV) is a respiratory, viral, contagious disease of equines and the caused by a virus of the genus Arterivirus (EVA). The disease is characterized by lesions in small vessels, especially arterioles. The disease usually runs as subclinical or acute. EAV infection can cause flu-like symptoms or respiratory symptoms in adult horses, abortion in pregnant mares, interstitial pneumonia in young foals, and persistent infection in stallions. Infection is possible only in equines and it is transmitted horizontally, either by aerosol or respiratory infection of susceptible horses or in natural or artificial insemination of mares with persistently infected stallions. Since serological tests have been developed serum neutralization test (SN), indirect immunofluorescence, agar gel immunodiffusion and ELISA test, and is currently the most commonly used are ELISA and SN test (Timoney, 2008; OIE 2008). In this study blood samples were collected from seven mares, which are used for mating. Serum neutralization test showed that there are 5 animals with a positive finding which is 71.43% of positive animals in the herd. Animals that have a positive antibody titers were repeatedly thought alike, all animals had reproductive problems but only one head came to abortion. Animals that have negative findings were first thought alike. For them, even though they stick together with other throats, there was no horizontal transmission of the virus. Positive animals exhibit some reproductive problems that can, but not exclusively, be attributed to this virus.
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