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Journal of Agricultural Sciences (Belgrade)
2016, vol. 61, br. 3, str. 247-256
jezik rada: engleski
vrsta rada: izvorni naučni članak
doi:10.2298/JAS1603247K

Creative Commons License 4.0
Izolacija i karakterizacija bakterija i kvasaca iz kontaminiranog zemljišta
aUniverzitet u Beogradu, Poljoprivredni fakultet
bUniverzitet EDUCONS, Fakultet zaštite životne sredine, Sremska Kamenica
cUniverzitet u Prištini (Kosovska Mitrovica), Prirodno-matematički fakultet

e-adresa: karlicicvera@gmail.com

Projekat

Biodiverzitet kao potencijal u ekoremedijacionim tehnologijama oštećenih ekosistema (MPNTR - 31080)
AREA - Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7 - 316004)

Sažetak

Zemljište predstavlja dinamičan ekosistem naseljen brojnim mikroorganizmima među kojima su bakterije najbrojnije. Najveći broj je skoncentrisan u uskoj zoni zemljišta koja okružuje koren i koja se naziva rizosfera. Procenjuje se da 1-2% bakterija koje naseljavaju ovu zonu imaju sposobnost da promovišu rast biljaka (engl. plant growth promoting bacteria - PGPB). Pored bakterija, sve više je podataka da i zemljišni kvasci poseduju ove sposobnosti. Mikroorganizmi koji stimulišu rast biljaka koriste različite mehanizme kojima povećavaju dostupnost nutrijenata biljkama, regulišu njihov hormonski status kao i odnos prema biljnim patogenima. Bakterije i kvasci koji stimulišu rast biljaka igraju važnu ulogu i u procesima bioremedijacije. Trideset bakterijskih i deset izolata kvasaca je izolovano iz kontaminiranog zemljišta (PAH i PCB) i testirano na prisustvo mehanizama kojima se pospešuje rast biljaka. Cilj je bio doći do sojeva koji su prilagođeni na život u zagađenom zemljištu, a istovremeno imaju i potencijalno stimulativno dejstvo. Nakon izvršenih biohemijskih analiza (produkcija amonijaka, indol-sirćetne kiseline, siderofora, rastvaranje neorganskog fosfora) u stimulatore biljnog rasta svrstana su tri bakterijska (Serratia liquefaciens, Micrococcus sp. i Serratia sp.) i dva izolata kvasaca (Candida utilis i Candida tropicalis). Među njima najveću sposobnost produkcije indol-sirćetne kiseline je pokazao izolat Serratia sp. (25,5 μg/ml). Analize tolerancije na prisustvo teških metala (Cu+2, Cr+6 i Ni+2) pokazale su da su izolati Serratia liquefaciens, Micrococcus sp., Serratia sp. i Candida tropicalis sposobni da podnesu više koncentracije. Rezultati ovih istraživanja mogu imati praktičnu primenu u budućim remedijacionim aktivnostima i unapređenju kvaliteta životne sredine.

Ključne reči

stimulacija rasta biljaka; mikroorganizmi; teški metali

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