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2020, vol. 36, br. 4, str. 437-445
Studija povezanosti starosti ovnova i kvaliteta njihovih ejakulata dobijenih van sezone parenja
BAS, Institute of Biology and Immunology of Reproduction "Acad. K. Bratanov", Sofia, Bulgaria

e-adresamadlena_andreeva_@abv.bg
Projekat:
The financial support of National Scientific Program for young scientists and postdoctoral fellows, funded by the Bulgarian Ministry of Education and Science (MES) with RMS № 577 (17.08.2018)

Ključne reči: ovan; spermatozoidi; pokretljivost; LDH; GGT
Sažetak
Cilj ove studije bui je da se istraži uticaj starosti na kvalitet ejakulata ovnova iz sintetičke populacije bugarske mlečne rase. Za eksperiment su korišćeni ovnovi podeljeni u dve starosne grupe: G1 (2,5 godine) i G2 (6 godina). Ejakulati su dobijeni metodom veštačke vagine i razblaženi korišćenjem medijuma 6A-G. Testirani ejakulati dobijeni su van sezone parenja. Analizator klase sperme (Sperm Class Analyzer, SCA Microptic, Barselona, Španija) korišćen je za proučavanje pokretljivosti sperme. Biohemijska aktivnost enzima laktat dehidrogenaze (LDH) i gama-glutamil transferaze (GGT) određena je pomoću automatskog spektrofotometra Mindrai BA88. Aktivnost enzima proučavana je u plazmi sperme, u vodenom i tritonskom ekstraktu. Pronađene su značajne razlike između grupa u ukupnoj pokretljivosti sperme (P≤0,05) i aktivnosti enzima LDH u plazmi sperme (P≤0,01). Što se tiče efikasnosti LDH enzima u vodenom ekstraktu i ekstraktu tritona, nisu pronađene značajne razlike između grupa. Nisu pronađene značajne razlike između grupa za aktivnost GGT, dok je starost imala značajan uticaj na LDH enzim u plazmi sperme.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/BAH2004437A
primljen: 15.10.2020.
prihvaćen: 19.11.2020.
objavljen u SCIndeksu: 06.01.2021.
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