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2019, vol. 70, br. 1, str. 28-33
Produkcija biofilma i pokretljivost po tipu trzanja i rojenja kliničkih izolata Acinetobacter baumannii
aUniverzitet u Beogradu, Medicinski fakultet
bUniverzitet u Banjoj Luci, Medicinski fakultet, Katedra za medicinsku mikrobiologiju, Republika Srpska, BiH
cUniverzitet u Beogradu, Medicinski fakultet, Institut za mikrobiologiju i imunologiju

e-adresaina.gajic@med.bg.ac.rs
Ključne reči: Acinetobacter baumannii; biofilm; pokretljivost; pili
Sažetak
Uvod: Acinetobacter baumannii je ubikvitaran gram negativan bacil, koji zahvaljujući multirezistenciji I preživljavanju na neživim objektima u vidu biofilma, postaje sve češći uzročnik nozokomijalnih infekcija. Bakterije roda Acinetobacter poseduju pile koji imaju značajnu ulogu u adherenciji, ali I u pokretljivosti bakterija po tipu trzanja I rojenja. Cilj: Cilj ovog rada je bio da se utvrdi sposobnost produkcije biofilma I pokretljivosti po tipu trzanja I rojenja kroz hranljivu podlogu kod sojeva a. baumannii izolovanih iz različitih kliničkih uzoraka. Materijal I metode: Ukupno je testirano 128 izolata a. baumannii poreklom iz krvi (34), briseva rana (35), respiratornog trakta (39) I ostalih kliničkih materijala (20). Izolatima je ispitana sposobnost produkcije biofilma u mikrotitarskoj ploči na 26°C I 37°C tokom 24 sati I na 37°C tokom 48 sati. Prisustvo pila je ispitano testiranjem pokretljivosti po tipu trzanja I rojenja kroz polučvrstu hranljivu podlogu. Rezultati: Preko 90% ispitivanih izolata je pokazalo umerenu ili izraženu sposobnost produkcije biofilma, nezavisno od uslova testiranja, pri čemu je sa produženjem vremena inkubacije uočeno smanjenje učestalosti jakih produktora biofilma (p < 0,001). Tokom 24-časovne inkubacije otkriveno je da su izolati iz rana I respiratornog trakta bili značajno češći u grupi jakih produktora biofilma u odnosu na izolate iz krvi I ostalih uzoraka. Nije uočena povezanost između specifičnih tipova pokretljivosti I produkcije biofilma. Zaključak: Produkcija biofilma izolata a. baumannii je u ispitivanim uslovima bila relativno stabilna fenotipska osobina. Nije uočena povezanost kretanja po tipu trzanja I rojenja ispitivanih uzoraka sa produkcijom biofilma.
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O članku

jezik rada: srpski
vrsta rada: izvorni naučni članak
DOI: 10.5937/mp70-17903
objavljen u SCIndeksu: 24.05.2019.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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