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2015, vol. 31, br. 1, str. 85-100
Skladištenje, čuvanje zamrznute sperme na farmama muznih krava
aUnidad de Reproducción y Obstetricia, Universidad de Santiago de Compostela (USC), Facultad de Veterinaria, Departamento de Patología Animal, Lugo Spain
bUniversidade Federal do Rio Grande do Sul (UFRGS), Facultade de Veterinária, Porto Alegre, Brasil

e-adresarodrigomuino@colvet.es
Ključne reči: motility; sperm subpopulations; management
Sažetak
Ukupno 26 kontejnera sa tečnim azotom je izabrano sa različitih farmi muznih krava. Tri doze semena su stavljene u kontejner/kanister koji se često koristi, dok je još tri slamke položeno u drugi kanister koji nije sadržavao nijednu dozu sperme, da se utvrdi da li se dopunjavanje sa tečnim azotom radi na odgovarajući način. Zatim, 10 dozi sperme koje pripadaju istoj zamrznutoj partiji su čuvane u našoj laboratoriji pod idealnim uslovima, i koristile su se kao kontrolne doze. Doze su posle određenog vremensko perioda prikupljene sa farmi i analizirane kako bi se dobili podaci o ukupnom motilitetom sperme i pojedinačnim kinetičkim parametrima svake sperme. Četiri subpopulacije sperme (SP) sa različitim obrascima pokretljivosti su identifikovani pomoću multivariacione analize klastera. Rezultati pokazuju da je srednja ukupna pokretljivost sperme neznatno opala za doze uskladištene u kontejneru/kanisteru koji se često koristi (45,2 ± 6,9%) u odnosu na doze pohranjene u kontejner/kanister koji se retko koristi (46,9 ± 59,0). Međutim, smanjenje ukupnog motiliteta bilo je veće u poređenju sa kontrolnim dozama (59,0%). Što se tiče sperme SP, (SP4 brza i progresivna sperma) koja je sadržala 31% od ukupnog broja spermatozoida (kontrolne doze), razlikovala se najviše kada su kontrolne doze poređene sa dozama koje se čuvaju u kontejnerima na farmama. Procenat potonjih je smanjen na 10% nakon što su skladištene u kontejnerima na farmama u periou od 7 meseci. Takva oštećenje kod SP 4 je progresivno i kumulativno i verovatno će doći do drastičnog smanjenja, što ugrožava plodnost navedenih doza sperme.
Reference
Abaigar, T., Holt, W.V., Harrison, R.A., del Barrio, G. (1999) Sperm subpopulations in boar (Sus scrofa) and gazelle (Gazella dama mhorr) semen as revealed by pattern analysis of computer-assisted motility assessments. Biology of reproduction, 60(1): 32-41
Barth, A.D. (1993) Factors affecting fertility with artificial insemination. u: Female bovine infertility, Vet. Clin. North. Anim. Food. Anim. Pract. 9 2, 275- 289
Berndtson, W.E., Picket, B.W., Rugg, C.D. (1976) Procedures for field handling of bovine semen in plastic straws. u: 6 th Tech. Conf. Artif. Insem. Reprod. Nat. Assoc. Anim. Breeders, Proc, 51-60
Berndtson, W.E., Foote, R.H. (1972) Bull sperm survival following freezing in ampoules, pellets and straws. u: 7th Inter Congr Anim Reprod Artif Insem, 2, 1353
Coulter, G.H., Foote, R.H. (1973) Sperm changes during processing in straws. J. Anim. Sci, 37, 306
Dalton, J.C. (2002) Semen handling: The forgotten link in the reproductive efficiency. West. Dairy News, 2 2, 35-36
de Jarnette, J.M., Saacke, R.G., Bame, J., Vogler, C.J. (1992) Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle. Journal of animal science, 70(2): 484-91
Ferraz, M.A.M.M., Morató, R., Yeste, M., Arcarons, N., Pena, A.I., Tamargo, C., Hidalgo, C.O., Muiño, R., Mogas, T. (2014) Evaluation of sperm subpopulation structure in relation to in vitro sperm-oocyte interaction of frozen-thawed semen from Holstein bulls. Theriogenology, 81(8): 1067-72
García-Ispierto, I., López-Gatius, F., Santolaria, P., Yániz, J.L., Nogareda, C., López-Béjar, M. (2007) Factors affecting the fertility of high producing dairy herds in northeastern Spain. Theriogenology, 67, 632-8
Hansen, P.J., Arechiga, C.F. (1999) Strategies for managing reproduction in the heat-stressed dairy cow. J Anim Sci, 77 Suppl 2 36-50
Holt, C., Holt, W.V., Moore, H.D. (1996) Choice of operating conditions to minimize sperm subpopulation sampling bias in the assessment of boar semen by computer-assisted semen analysis. Journal of andrology, 17(5): 587-96
Holt, W.V. (2000) Basic aspects of frozen storage of semen. Animal reproduction science, 62(1-3): 3-22
Janett, F., Schilter, E., Weber, F., Witschi, U., Thun, R. (2008) Effects of straw handling during storage on semen quality in the bull. Schweiz Arch. Tierheilkd, 150 12, 591-597
Johnson, M.S., Senger, P.L., Allen, C.H., Hancock, D.D., Alexander, B.M., Sasser, R.G. (1995) Fertility of bull semen packaged in .25- and .5-milliliter french straws. Journal of animal science, 73(7): 1914-9
Larson, G.L., Graham, E.F. (1958) Effects of low temperatures in storage of bovine semen. AI Digest, 6, 6
Lineweaver, J.A., Saake, R.G., Gwasdauskas, F.C. (1979) Effect of double decking and storage time on semen stored in farm storage tanks. u: Am. Dairy Sci. Assoc, Proc, 175
López-Gatius, F. (2003) Is fertility declining in dairy cattle? A retrospective study in northeastern Spain. Theriogenology, 60(1): 89-99
Lucy, M.C. (2001) Reproductive loss in high-producing dairy cattle: where will it end?. Journal of dairy science, 84(6): 1277-93
Lucy, M.C. (2003) Mechanisms linking nutrition and reproduction in postpartum cows. Reprod Suppl, 61: 415-27
Martínez, I.N., Morán, J.M., Peña, F.J. (2006) Two-step cluster procedure after principal component analysis identifies sperm subpopulations in canine ejaculates and its relation to cryoresistance. Journal of andrology, 27(4): 596-603
Mortimer, S.T. (1997) A critical review of the physiological importance and analysis of sperm movement in mammals. Human reproduction update, 3(5): 403-39
Muiño, R., Tamargo, C., Hidalgo, C.O., Peña, A.I. (2008) Identification of sperm subpopulations with defined motility characteristics in ejaculates from Holstein bulls: Effects of cryopreservation and between-bull variation. Animal Reproduction Science, 109(1-4): 27-39
Nadir, S., Saacke, R.G., Bame, J., Mullins, J., Degelos, S. (1993) Effect of freezing semen and dosage of sperm on number of accessory sperm, fertility, and embryo quality in artificially inseminated cattle. Journal of animal science, 71(1): 199-204
Pace, M.M., Sullivan, J.J. (1978) A biological comparison of the O.5 ml ampule and O.5 ml French straw systems for packaging bovine spermatozoa. u: 7th Tech. Conf. Artif. Insem. Reprod. Nat. Assoc. Anim. Breeders, Proc, 3-9
Pickett, B.W. (1971) Factors affecting the utilization of frozen bovine semen for maximum reproductive efficiency. A.I. Digest, 19 2, 8
Pickett, B.W., Berndtson, W.E. (1974) Preservation of Bovine Spermatozoa by Freezing in Straws: A Review. Journal of Dairy Science, 57(11): 1287-1301
Quintela, L.A., Díaz, C., Becerra, J., Herradon, P., Angeles, P., Gallego, N. (1997) Importancia de los minerales y las vitaminas en la reproducción del ganado vacuno. Albeitar, 12-14
Quintero-Moreno, A., Miró, J., Teresa, R.A., Rodríguez-Gil, J.E. (2003) Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenology, 59(9): 1973-90
Rapatz, G. (1966) What happens when semen is frozen. u: 1st. Tech. Conf. Artif. Insem. Bovine Reprod. Nat. Assoc. Anim. Breeder, Proc, 45
Roche, J.F., Mackry, D., Diskin, M.D. (2000) Reproductive menagement of postpartum cows. Animal Reproduction Science, 60-61, 703-712
Saacke, R.G. (1983) Semen quality in relation to semen preservation. Journal of dairy science, 66(12): 2635-44
Senger, P.L. (1980) Handling frozen bovine semen - Factors which influence viability and fertility. Theriogenology, 13(1): 51-62
Stevenson, J.S., Higgins, J.J., Jung, Y. (2009) Pregnancy outcome after insemination of frozen-thawed bovine semen packaged in two straw sizes: A meta-analysis. Journal of Dairy Science, 92(9): 4432-4438
 

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/BAH1501085M
objavljen u SCIndeksu: 24.06.2015.