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2015, vol. 31, br. 1, str. 85-100
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Skladištenje, čuvanje zamrznute sperme na farmama muznih krava
Management of the storage of cryopreserved sperm on dairy cattle farms
aUnidad de Reproducción y Obstetricia, Universidad de Santiago de Compostela (USC), Facultad de Veterinaria, Departamento de Patología Animal, Lugo Spain bUniversidade Federal do Rio Grande do Sul (UFRGS), Facultade de Veterinária, Porto Alegre, Brasil
e-adresa: rodrigomuino@colvet.es
Sažetak
Ukupno 26 kontejnera sa tečnim azotom je izabrano sa različitih farmi muznih krava. Tri doze semena su stavljene u kontejner/kanister koji se često koristi, dok je još tri slamke položeno u drugi kanister koji nije sadržavao nijednu dozu sperme, da se utvrdi da li se dopunjavanje sa tečnim azotom radi na odgovarajući način. Zatim, 10 dozi sperme koje pripadaju istoj zamrznutoj partiji su čuvane u našoj laboratoriji pod idealnim uslovima, i koristile su se kao kontrolne doze. Doze su posle određenog vremensko perioda prikupljene sa farmi i analizirane kako bi se dobili podaci o ukupnom motilitetom sperme i pojedinačnim kinetičkim parametrima svake sperme. Četiri subpopulacije sperme (SP) sa različitim obrascima pokretljivosti su identifikovani pomoću multivariacione analize klastera. Rezultati pokazuju da je srednja ukupna pokretljivost sperme neznatno opala za doze uskladištene u kontejneru/kanisteru koji se često koristi (45,2 ± 6,9%) u odnosu na doze pohranjene u kontejner/kanister koji se retko koristi (46,9 ± 59,0). Međutim, smanjenje ukupnog motiliteta bilo je veće u poređenju sa kontrolnim dozama (59,0%). Što se tiče sperme SP, (SP4 brza i progresivna sperma) koja je sadržala 31% od ukupnog broja spermatozoida (kontrolne doze), razlikovala se najviše kada su kontrolne doze poređene sa dozama koje se čuvaju u kontejnerima na farmama. Procenat potonjih je smanjen na 10% nakon što su skladištene u kontejnerima na farmama u periou od 7 meseci. Takva oštećenje kod SP 4 je progresivno i kumulativno i verovatno će doći do drastičnog smanjenja, što ugrožava plodnost navedenih doza sperme.
Abstract
26 liquid nitrogen tanks were selected from different dairy cattle farms. Three sperm doses were introduced in a frequently used canister, while another three straws were deposited in another canister that did not contain any sperm doses, to determine whether the refilling with liquid nitrogen had been done appropriately. Then, 10 sperm doses belonging to the same freezing lot were stored in our laboratory under ideal conditions to be used as control doses. After certain time period, the doses were collected from the farms and were analysed to obtain data about their total sperm motility and the individual kinetic parameters of each sperm. Four sperm subpopulations (SP) with different patterns of motility were identified using a cluster multivariate analysis. The results show that the mean total sperm motility has hardly decreased for the doses stored in the frequently used canister (45.2 ± 6.9%) in comparison with the doses stored in the rarely used canister (46.9 ± 59.0). However, the decrease in total motility was greater when compared with the control doses (59.0%). As for the sperm SP, (SP4 rapid and progressive sperm) which contained 31% of the total of sperm (control doses), differed the most when control doses were compared to straw stored in farm tanks. The percentage of the latter was reduced to 10 % after being stored in the tanks of the farms for 7 mo. Such damage in SP 4 is progressive and cumulative and would probably reduce drastically compromising the fertility of the aforementioned sperm doses.
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