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Hemijska industrija
2013, vol. 67, br. 3, str. 411-418
jezik rada: engleski
vrsta rada: naučni članak
objavljeno: 02/09/2013
doi: 10.2298/HEMIND120607093S
Oksidacija kvercetina pomoću peroksidaze iz rena - efekat UV-B zračenja
Univerzitet u Nišu, Tehnološki fakultet, Leskovac

e-adresa: zpetronijevic@yahoo.com

Projekat

Biljni i sintetički bioaktivni proizvodi novije generacije (MPNTR - 34012)
Dobijanje, fizičko-hemijska karakterizacija, analitika i biološka aktivnost farmakološki aktivnih supstanci (MPNTR - 172044)

Sažetak

Peroksidaza iz rena (HRP) jedan je od najviše istraživanih enzima iz grupe peroksidaza, koji poseduje pored drugih bioloških aktivnosti i sposobnost da katalizuje oksidaciju mnogih flavonoida i fenolnih supstrata, između ostalog i kvercetina. Sa druge strane, poznato je da kvercetin poseduje izraženu antioksidativnu aktivnost, koja u slučaju UV zračenja može biti izražena delom i na preventivni način: kvercetin je odličan UV-absorber. Stoga je cilj ovog istraživanja bio da se ispita oksidacija kvercetina pomoću peroksidaze iz rena u fosfatnom puferu pod uslovima UV-stresa, tj. pod dejstvom kontinualnog produženog UV-B ozračivanja. Rezultati pokazuju da definitivno, UV-B ozračivanje utiče na aktivnost HRP, kao i na ukupnu brzinu oksidacije kvercetina pomoću HRP, s tim da je uticaj na brzinu oksidacije (kvercetina) veoma mali nakon dužeg perioda UV-B ozračivanja (>30 min).

Ključne reči

Kvercetin; Peroksidaza UV-B zračenje; Kinetika; Oksidacija

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