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Journal of Medical Biochemistry
2016, vol. 35, br. 3, str. 319-323
jezik rada: engleski
vrsta rada: izvorni naučni članak
objavljeno: 02/02/2020
PMC ID: PMC5346810
doi: 10.1515/jomb-2016-0008
Polimorfizmi NLRP3 i CARD8 utiču na pojačanu aktivnost bolesti u reumatoidnom artritisu
aUniversity of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Pharmacogenetics Laboratory, Ljubljana, Slovenia
bUniversity Medical Centre Ljubljana, Department of Rheumatology, Ljubljana, Slovenia + University of Ljubljana, Faculty of Medicine, Ljubljana, Slovenia

e-adresa: vita.dolzan@mf.uni-lj.si

Projekat

This work was financially supported by the Slovenian Research Agency (ARRS Grants No. P1-0170)

Sažetak

Uvod: Aktivacija NLRP3-inflamazoma može pojačati zapaljenske procese u reumatoidnom artritisu (RA). Funkcionalni polimorfizmi u genima koji kodiraju za njegove komponente NLRP3 i CARD8 dovedeni su u vezu sa proinflamatornim fenotipom. Naš cilj bio je da istražimo uticaj ovih polimorfizama na podložnost RA-u i aktivnost bolesti u vreme dijagnoze i posle šest meseci. Metode: U grupi od 128 obolelih od RA-a koji su lečeni metotreksatom kao i kod 122 zdravih kontrolnih ispitanika urađena je genotipizacija za polimorfizme NLRP3 rs35829419 (p. Q705K) i CARD8 rs2043211 (p. C10X). Rezultati: Na podložnost RA-u nisu uticali istraživani polimorfizmi niti njihova interakcija. Istraživani polimorfizmi objašnjavaju 8% varijabilnosti u DAS28 u vreme dijagnoze. Nosioci polimorfizama NLRP3 rs35829419 ili CARD8 rs2043211 imali su značajno viši DAS28 u vreme dijagnoze (p=0,003, odnosno p=0,022). Polimorfni genotip CARD8 rs2043211 TT takođe je bio povezan sa višim DAS28 posle šest meseci lečenja (p=0,033). Zaključak: arijabilnost komponenti inflamazoma može dovesti do pojačane aktivnosti bolesti u vreme dijagnoze i posle šest meseci lečenja metotreksatom kod obolelih od RA-a. Bolje razumevanje imunoloških mehanizama koji stoje iza aktivnijeg toka RA-a moglo bi ukazati na nove pristupe u lečenju u podskupu pacijenata sa proinflamatornim fenotipom.

Ključne reči

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