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2020, vol. 61, br. 1, str. 44-51
In vitro ispitivanje antioksidativne aktivnosti preparata ruzmarinske kiseline i meda
aHealthcare center "Sloga Medik", Kruševac
bUniverzitet u Beogradu, Farmaceutski fakultet, Institut za medicinsku biohemiju
cUniverzitet u Beogradu, Farmaceutski fakultet, Institut za bromatologiju
dSvijet biljaka d.o.o, Vodnjan, Croatia

e-adresamiron.sopic@pharmacy.bg.ac.rs
Sažetak
Usled narušavanja ravnoteže između slobodnih radikala i antioksidanasa, dolazi do oksidativnog stresa, patofiziološkog stanja, koje je povezano sa nastankom i razvojem mnogih oboljenja. Kako su se određeni sintetski antioksidansi pokazali kao potencijalno štetni po zdravlje ljudi, sve se više pažnje usmerava ka pronalaženju prirodnih antioksidanasa. Polifenoli, fenolne kiseline, u koje spada i ruzmarinska kiselina, terpeni i terpenoidi su sekundarnii metaboliti biljaka koji poseduju antioksidativne osobine, a mogu se naći u različitim biljnim kompleksima (hidrolatima) i medu. Cilj ovog rada je utvrđivanje in vitro antioksidativne aktivnosti preparata koji predstavlja mešavinu biljnih kompleksa (hidrolata), ruzmarinske kiseline i meda. U "pool" seruma, koji je dobijen mešanjem seruma zdravih pojedinaca, dodavana su serijska razblaženja (100%, 50%, 25%) ispitivanog preparata, kao i serijska razblaženja ispitivanog preparata (100%, 50%, 25%) u prisustvu terc-butil hidroperokisda (TBH), kao prooksidansa. Pored ovih uzoraka, pripremljeni su i uzorci u koje je dodavan troloks (hidrofilni analog vitamina E), kao i troloks u prisustvu TBH. Iz svih uzoraka vršeno je određivanje parametara antioksidativnog stresa: totalnog antioksidativnog statusa (TAS), paraoksonaze-1 (PON1), ukupnog sadržaja sulfhidrilnih grupa (SHG), totalnog oksidativnog statusa (TOS), prooksidativnog-antioksidativnog balansa (PAB) i uznapredovalih produkata oksidacije proteina (AOPP). Takođe, izračunat je i odnos TAS/TOS koji predstavlja kvantitativnu meru odnosa antioksidativnih redukujućih supstanci i oksidujućih supstanci u serumu. Poređenjem vrednosti antioksidativnih parametara TAS, SHG, TAS/ TOS odnosa i parametara ravnoteže prooksidanasa i antioksidanasa PAB za uzorke preparata bez i sa dodatkom TBH, nije uočena značajna razlika, što ukazuje da je i pored dodatka TBH ispitivani preparat održavao svoje antioksidativno delovanje. Ispitivani preparat pokazao je značajno antioksidativno delovanje, što se može pripisati sinergističkom delovanju terpena i terpenoida iz hidrolata, ruzmarinske kiseline, polifenola i ostalih antioksidativnih supstanci iz meda.
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O članku

jezik rada: engleski
vrsta rada: izvorni članak
DOI: 10.5937/hraIsh2001044M
primljen: 10.12.2020.
prihvaćen: 30.12.2020.
objavljen u SCIndeksu: 06.03.2021.

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