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Medicinski časopis
2013, vol. 47, br. 2, str. 74-79
jezik rada: srpski
vrsta rada: prethodno saopštenje
doi:10.5937/mckg47-1794
Poređenje imunohemijskih metoda za određivanje koncentracije srčanog troponina i kod dva analizatora
KBC Zvezdara

e-adresa: katarina.ille@gmail.com

Sažetak

Cilj. Srčani troponin I je biohemijski pokazatelj oštećenja miokarda koji se godinama koristi u Kliničko-bolničkom centru 'Zvezdara' u Beogradu. Za određivanje koncentracije troponina I u serumu pacijenata koristi se hemiluminiscentni imunoesej (Siemens Immulite 1000). S ciljem ubrzanja dobijanja rezultata razmatrano je uvođenje nove metode za određivanje kardijalnog troponina I, elektrohemiluminiscentnog imunoeseja, korišćenjem analizatora tipa Roche Cobas e411. Metode. Ispitana je nova, kandidat metoda za određivanje koncentracije troponina I pre eventualnog uvođenja u kliničku praksu i upoređena je sa metodom koja se već koristi. Poređenje koncentracija srčanog troponina I dobijenih upotrebom metoda Roche Cobas e411 analizatora (x) sa Siemens Immulite 1000 analizatora (y) izvedeno je kod uzoraka seruma 94 odrasla pacijenta, oba pola, sa znacima i simptomima akutnog infarkta miokarda. Rezultati. Analizom rezultata ustanovljena je jednačina regresije y = 1,91x + 4,67; koeficijent korelacije iznosio je 0,92. U 15 od 94 pacijenta (16%) rezultati istog uzorka dobijeni dvema metodama nisu se slagali. Kod takvih osoba rezultat troponina I dobijen jednom metodom ukazivao je na prisustvo akutnog infarkta miokarda ali kod druge, on je bio ispod dijagnostičke pragovne vrednosti. Zaključak. Između vrednosti koncentracija troponina I izmerenih poređenim metodama ne postoji dobra saglasnost, izraženo kroz koeficijent korelacije i jednačinu regresije. Interpretacija rezultata bar kod svakog šestog pacijenta upućivala bi na suprotne zaključke u dijagnozi akutnog koronarnog događaja.

Ključne reči

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