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Serbian Journal of Experimental and Clinical Research
2010, vol. 11, br. 4, str. 147-155
jezik rada: engleski
vrsta rada: izvorni naučni članak
Uticaj bazalne vrednosti superoksid dismutaze na balans pro/antioksidanasa izazvan akutnim fizičkim naporom
Katedra za fiziologiju, Medicinski fakultet, Kragujevac

Sažetak

Superoksid dismutaza (SOD), prva linija antioksidativnog zaštitnog sistema, enzim je koji je u prethodnim istraživanjima bio najpodložniji promenama usled uticaja trenažnog procesa. Ovaj enzim je takođe bio onaj po kom su se dobro utrenirani i netrenirani ispitanici istraživanja razlikovali. Cilj naše studije bio je utvrđivanje razlike u odgovoru parametara redoks ravnoteže na jednokratno fi zičko vežbanje kod ispitanika sa različitom bazalnom aktivnosti SOD. 24 mlada rukometaša podvrgnuta su maksimalnom progresivnom testu opterećenja. Neposredno pre i nakon testa opterećenja ispitanicima su uzeti uzorci krvi, radi biohemijske analize parametara redoks ravnoteže - u plazmi je određivan nivo superoksid anjon radikala (O2-), hidrogen peroksida (H2O2), azotnog monoksida (NO, određivanog preko nitrita NO2-), indeksa lipidne peroksidacije (TBARS), a u eritrocitima aktivnost superoksid dismutaze (SOD) i katalaze (CAT). Jednokratno vežbanje izazvalo je statistički značajne promene svih praćenih parametara, osim O2-, a najznačajnije promene uočene su u grupi ispitanika sa najnižom bazalnom aktivnosti SOD. Značajnije korelacije pronađene su između bazalne aktivnosti SOD i H2O2, i SOD i NO (NO2-). Ovi rezultati navode nas na zaključak da bazalna aktivnost SOD određuje efekte vežbanja na redoks ravnotežu.

Ključne reči

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