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Acta veterinaria
2008, vol. 58, br. 2-3, str. 129-137
jezik rada: engleski
vrsta rada: neklasifikovan
doi:10.2298/AVB0803129N


Antioksidativna zaštita u moždanom stablu i talamusu u eksperimentalnoj sepsi
aVojnomedicinska akademija
bNaučni institut za veterinarstvo 'Novi Sad', Novi Sad
cDepartment of Toxicology, Faculty of Pharmacy, Belgrade

e-adresa: vmaimi@EUnet.yu

Sažetak

Moždane komplikacije u toku sepse nisu retke, ali patofiziološki događaji u nekim delovima mozga još uvek nisu razjašnjeni. U ovom radu su razmatrani rezultati dobijeni određivanjem antioksidativne komponente - aktivnost glutation peroksidaze (GSHPx) i koncentracije redukovanog glutationa (GSH) u integrativnim moždanim centrima, moždanom stablu i talamusu. Odraslim Wistar pacovima, mužjacima (200-250 g) sepsa je izazivana cekalnom ligacijom i perforacijom (CLP) uz inokulaciju suspenzije Escherichia coli (ATCC 25922) (n=40). Kontrolne životinje su bile lažno operisane (n=40). U vremenski određenim terminima (0, 12, 24 i 72 časa) nakon tretmana, po deset životinja iz svake grupe je bilo dekapitovano. U moždanom stablu, aktivnost GSHPx povećala se u 12. i 24. času nakon CLP, dok se u talamusu, aktivnost GSHPx povećala u 72. času u odnosu na kontrolu. U moždanom stablu, koncentracija GSH smanjila se u 12. i 24. satu, a u talamusu se smanjila u 72. satu, u odnosu na kontrolu. Promenjen oksidativni status u moždanom stablu, već nakon 12 h od CLP pokazuje brzu reaktivnost centralnog nervnog sistema, što bi mogao da bude značajan faktor poremećaja vazo-motornog odgovora u sepsi.

Ključne reči

brain stem; glutathione; glutathione peroxydase; rat; sepsis; thalamus

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