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2018, vol. 34, br. 1, str. 49-58
Potencijal ekstrakta listova Telfairia occidentalis kao ekstendera semena nerastova
aBowen University, Department of Animal sciences and Fisheries management, Iwo, Osun state, Nigeria + University of Ibadan, Department of Animal sciences, Oyo state, Nigeria
bUniversity of Ibadan, Department of Animal sciences, Oyo state, Nigeria
cBowen University, Department of Animal sciences and Fisheries management, Iwo, Osun state, Nigeria

e-adresaayoolamatt@gmail.com
Ključne reči: ekstender semena; nerast; pH; Telfairia occidentalis; rastvor za otapanje Beltsville
Sažetak
Čuvanje semena za veštačku inseminaciju (AI) zahteva korišćenje ekstendera, koji je služio kao puferski medijum za zaštitu i očuvanje ćelija sperme u dobrom stanju za oplodnju. U ovoj studiji, poređen je potencijal ekstrakta lista Telfairia occidentalis (TOLE) kao nekonvencionalnog ekstendera semena nerastova sa rastvorom za otapanje Beltsville (BTS), konvencionalnim ekstenderom semena tokom perioda od 48 časova skladištenja. Vodeni rastvor TOLE-a je pripremljen u tri (3) nivoa koncentracije (25, 50 i 75%). Sveži ejakulati nerastova su tretirani u (1: 4) TOLE rastvorima i BTS ekstenderu u potpuno slučajnom dizajnu; tretmani su ponovljeni četiri (4) puta. Tretirano seme je skladišteni na temperaturi od 170 C i ocenjivano je njegov kvalitet odn. pH, odnos mrtvih/živih (%) i koncentracija spermatozoida u vremenskim intervalima (0, 12, 24, 36 i 48h). Tretirano seme je bilo značajno (P<0,05) pod uticajem ekstendera semena i perioda skladištenja za pH, a procenat/udeo mrtvih/živih (%), ali i koncentracija spermatoznih ćelija (103) nisu bile pod uticajem faktora (P>0.05). Vrednosti pH za BTS su značajno (P <0.05) veće u poređenju sa TOLE ekstenderima. TOLE ekstender u koncentraciji od 75% je imao najveću vrednost (43,89%), a BTS je imao najmanju vrednost (12,15%) za odnos mrtvih/živi šti je statistički signifikantno (P <0,05). Vrednosti pH i odnos mrtvih/živih (%) povećane su tokom vremena čuvanja u svim ekstenderima. Optimalne performanse TOLE-a u poređenju sa BTS ekstendorom zabeležene su u koncentraciji TOLE-a od 50%. TOLE je pokazao potencijal kao ekstender semena nerastova, ali su potrebne dalje studije za validaciju i poboljšanje njegove primene.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/bah1801049A
objavljen u SCIndeksu: 13.07.2018.
Creative Commons License 4.0

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