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2018, vol. 34, iss. 1, pp. 49-58
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Potential of Telfairia occidentalis leaf extract as boar semen extender
Potencijal ekstrakta listova Telfairia occidentalis kao ekstendera semena nerastova
aBowen University, Department of Animal sciences and Fisheries management, Iwo, Osun state, Nigeria + University of Ibadan, Department of Animal sciences, Oyo state, Nigeria bUniversity of Ibadan, Department of Animal sciences, Oyo state, Nigeria cBowen University, Department of Animal sciences and Fisheries management, Iwo, Osun state, Nigeria
email: ayoolamatt@gmail.com
Abstract
The preservation of semen for Artificial insemination (AI) required the use of extender, which served as a buffer media to keep the sperm cell in good condition for fertilization. In this study, fluted pumpkin Telfairia occidentalis leaf extract (TOLE) potential as an unconventional boar semen extender was compared to Beltsville thawing solution (BTS), a conventional semen extender over a 48hr storage period. Aqueous solution of TOLE was prepared at three (3) concentration levels (25, 50 and 75%). Fresh ejaculates from boar were extended at (1:4) in TOLE solutions and BTS extender in a completely randomized design; treatments were replicated four (4) times. Extended semen was stored at 170 C and evaluated for semen qualities which include pH, dead/live ratio (%) and sperm cell concentration at time intervals (0, 12, 24, 36 and 48) hrs. Extended semen was significantly (P < 0.05) affected by semen extenders and storage periods for pH, and dead/live ratio (%) but sperm cell concentration (103 /cm3 ) not (P > 0.05) affected. The pH values for BTS was significantly (P < 0.05) higher as compared to TOLE extenders. 75% TOLE extender had highest value (43.89%), and BTS had lowest value (12.15%) for dead/live ratio significantly (P < 0.05). The pH and dead/live ratio (%) values increased over storage time in all extender. The optimum performance of TOLE as compared to BTS extender was recorded at 50% concentration of TOLE extender. TOLE showed a potential as boar semen extender, but further studies are required to validate and improve its application.
Sažetak
Čuvanje semena za veštačku inseminaciju (AI) zahteva korišćenje ekstendera, koji je služio kao puferski medijum za zaštitu i očuvanje ćelija sperme u dobrom stanju za oplodnju. U ovoj studiji, poređen je potencijal ekstrakta lista Telfairia occidentalis (TOLE) kao nekonvencionalnog ekstendera semena nerastova sa rastvorom za otapanje Beltsville (BTS), konvencionalnim ekstenderom semena tokom perioda od 48 časova skladištenja. Vodeni rastvor TOLE-a je pripremljen u tri (3) nivoa koncentracije (25, 50 i 75%). Sveži ejakulati nerastova su tretirani u (1: 4) TOLE rastvorima i BTS ekstenderu u potpuno slučajnom dizajnu; tretmani su ponovljeni četiri (4) puta. Tretirano seme je skladišteni na temperaturi od 170 C i ocenjivano je njegov kvalitet odn. pH, odnos mrtvih/živih (%) i koncentracija spermatozoida u vremenskim intervalima (0, 12, 24, 36 i 48h). Tretirano seme je bilo značajno (P<0,05) pod uticajem ekstendera semena i perioda skladištenja za pH, a procenat/udeo mrtvih/živih (%), ali i koncentracija spermatoznih ćelija (103) nisu bile pod uticajem faktora (P>0.05). Vrednosti pH za BTS su značajno (P <0.05) veće u poređenju sa TOLE ekstenderima. TOLE ekstender u koncentraciji od 75% je imao najveću vrednost (43,89%), a BTS je imao najmanju vrednost (12,15%) za odnos mrtvih/živi šti je statistički signifikantno (P <0,05). Vrednosti pH i odnos mrtvih/živih (%) povećane su tokom vremena čuvanja u svim ekstenderima. Optimalne performanse TOLE-a u poređenju sa BTS ekstendorom zabeležene su u koncentraciji TOLE-a od 50%. TOLE je pokazao potencijal kao ekstender semena nerastova, ali su potrebne dalje studije za validaciju i poboljšanje njegove primene.
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