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Acta veterinaria
2012, vol. 62, br. 1, str. 53-65
jezik rada: engleski
vrsta rada: neklasifikovan
doi:10.2298/AVB1201053J


Koncentracija malonil-dialdehida u krvnoj plazmi, biohemijski i hematološki parametri kod američkog kasača tokom odabranih testova opterećenja na terenu
aAgency for medicinal products and medical devices, Ljubljana, Slovenia
bUniversity of Ljubljana, Veterinary Faculty, Ljubljana, Slovenia
cUniversity of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia
dUniversity of Ljubljana, Veterinary Faculty, Institute of Physiology, Pharmacology and Toxicology, Ljubljana, Slovenia

e-adresa: sarajagric@gmail.com

Sažetak

Cilj ove studije je bio da se utvrde promene u koncentraciji malonil-dialdehida (MDA) u krvnoj plazmi kao i hematološki i biohemijski profil kod 10 klinički zdravih američkih kasača podvrgnutih odabranim testovima opterećenja na terenu. U krvnom serumu je određivan i odnos između koncentracije MDA, krajnjeg produkta glavne lipidne peroksidacije i aktivnosti enzima mišića, kreatin kinaze (CK) i aspartat aminotransferaze (AST). Uzorski venske krvi za analize su prikupljani u mirovanju, neposredno po završetku opterećenja a zatim i 24 i 48 sati nakon toga. Neposredno po završetku opterećenja, utvrđene su značajne promene većine biohemijskih i hematoloških parametara i one se mogu smatrati normalnim fiziološkim odgovorom. Većina ovih parametara se vratila na vrednosti registrovane u mirovanju u roku od 48 sati. Koncentracija MDA u krvnoj plazmi je odmah posle napora bila povećana, ali ovo povećanje nije bilo statistički značajno za razliku od vrednosti registrovanih 24 sata posle vežbe. Maksimalna vrednost MDA je registrovana nakon 48 sati. Ovaj oksidativni stres je bio indukovan opterećenjem što potvrđuje povećan stepen lipidne peroksidacije. Na osnovu brzog pada aktivnosti CK u serumu po završetku opterećenja i odsustvu značajne korelacije između koncentracije MDA i aktivnosti mišićnih enzima u serumu, može se zaključiti da odabrani testovi opterećenja nisu imali za posledicu trajne alteracije u mišićnim ćelijama i da nisu dovodili do njihovog oštećenja.

Ključne reči

biochemical parameters; exercise; haematological parameters; lipid peroxidation; muscle cell integrity; standardbred horses

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