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Medicinski časopis
2019, vol. 53, br. 2, str. 43-48
jezik rada: engleski
vrsta rada: izvorni naučni članak
doi:10.5937/mckg53-17381
Razlikovanje modaliteta ćelijske smrti leukocita korišćenjem protočne citometrije kod bolesnika sa hroničnim rinosinuzitisom bez nazalnih polipa
aKharkiv National Medical University, Department of Biochemistry, Kharkiv, Ukraine
bKharkiv National Medical University, Department of Otorhinolaryngology, Kharkiv, Ukraine
cInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Department of Cryocytology, Kharkiv, Ukraine

e-adresa: antontkachenko555@gmail.com

Sažetak

Cilj. Cilj našeg istraživanja bio je da se ispitaju brzina i modaliteti ćelijske smrti leukocita pomoću seta protočne citometrije aneksina V i 7-AAD kod pacijenata sa hroničnim rinosinuzitisom bez nazalnih polipa (CRSsNP). Metode. Za otkrivanje modaliteta ćelijske smrti leukocita kod dvanaest pacijenata sa CRSsNP korišćena je protočna citometrija. Za procenu faza apoptoze korišćeni su aneksin V FITC, anti-CD45 PE i 7-AAD (7-aminoaktinomicin D). Aktuelna metoda omogućava identifikovanje četiri različita stanja ćelija: 1 - vijabilne ćelije (aneksin V negativne, 7-AAD negativne ćelije); 2 - rane apoptotičke ćelije (aneksin V pozitivne, 7-AAD negativne ćelije); 3 - kasne apoptotičke/ nekrotične ćelije (aneksin V pozitivne, 7-AAD pozitivne ćelije); 4 - mrtve nekrotične ćelije (aneksin V negativne, 7-AAD pozitivne ćelije). Da bi se procenila stopa oksidativnog stresa u CRSsNP, određivane su koncentracije 8-izoprostana iz krvnog seruma pomoću ELISA. Rezultati. Ustanovljeno je da je CRSsNP praćen razvojem oksidativnog stresa, pokazano povišenjem 8-izoprostana u krvnom serumu i povećanom stopom ćelijske smrti leukocita sa dominacijom ranih apoptotskih ćelija, što potvrđuje i veći procenat aneksin V pozitivnih, 7-AAD negativnih ćelija u poređenju sa kontrolnim pacijentima. Zaključak. Nalazi studije sugerišu da CRSsNP prati aktivacija apoptoze leukocita.

Ključne reči

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