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Ratarstvo i povrtarstvo
2014, vol. 51, iss. 2, pp. 100-105
Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules
Dinić Zorana ORCID, Ugrinović Milanb ORCID, Bosnić Predragc ORCID, Mijatović Mirab, Zdravković Jasminab ORCID, Miladinović Miroslava, Jošić Draganaa ORCID
aInstitute of Soil Sciences, Belgrade, Serbia
bInstitute for Vegetable Crops, Smederevska Palanka, Serbia
cUniversity of Belgrade, Institute for Multidisciplinary Research, Serbia
Project: New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants (MESTD - 46007)
Keywords: endophytic bacteria; French bean nodules; phosphate solubilizing bacteria; Pseudomonas; soil pH
Abstract
Phosphorus (P) is one of the major essential macronutrient for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study endophytic bacteria were isolated form nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4. Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml-1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.
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About

article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov51-6222
published in SCIndeks: 12/12/2014
peer review method: double-blind

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