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2019, vol. 46, br. 1, str. 147-153
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Aspekti biohemijskih parametara krvne plazme i intestinalnog zdravlja odlučenih prasadi hranjenih mešavinom ekstrudiranog lanenog semena i sačme oraha
Aspects of plasma biochemistry and intestinal health of weaned piglets fed dietary extruded linseed and walnut meal mixture
Nacionalni istraživačko-razvojni institut za biologiju i ishranu životinja (INCDBNA), Balotešti, Kalea Bukurešti, Rumunija
e-adresa: anca.gheorghe@ibna.ro
Projekat: This study was funded by the Romanian Ministry of Research and Innovation through Nucleus Program, grant 1820 0103 and Subprogram 1.2. Institutional Performance, Program 1. Developing National R&D, National Research and Development and Innovation, Contract no.17 PFE/17.10.2018
Ključne reči: ekstrudirano hranivo; hranljiva smeša; enteritis; fekalna mikroflora; parametri krvne plazme; prasad
Keywords: extruded meal; dietary mixture; enteritis; fecal microflora; plasma parameters; piglets
Sažetak
U okviru ovog istraživanja ispitivan je uticaj ishrane prasadi smešom ekstrudiranog semena lana (ELS) i sačme oraha (WM) u odnosu 8:1 na odabrane parametre krvne plazme i zdravlja creva kod odlučenih jedinki. Četrdeset prasadi (Topigs hibrid) telesne mase (BW) 8,02 ± 0,82 kg, starosti 30 ± 3 dana, podeljeno je u 2 tretmana: kontrolni (C, ishrana na bazi kukuruza, tritikalea i sojine sačme (SBM)) i eksperimentalni (smešom u kojoj smeša ekstrudiranog semena lana i oraha, u odnosu 8:1, delimično zamenjuje SBM). Uzorci krvi su sakupljani 7-og i 21-og dana nakon odlučivanja prasadi (PW). Za određivanje lipida u krvnoj plazmi (ukupni holesterol, T-Chol; HDL-holesterol, HDL-C; trigliceridi, TG), mineralnog i enzimskog profila korišćen je hemijski analizator. Mikrobiološke populacije iz fekalnih uzoraka određivane su brojanjem kolonija dobijenih na odabranim medijumima. Na osnovu dobijenih rezultata nije ustanovljen efekat primenjene mešavine u ishrani odlučene prasadi (PW) na parametre krvne plazme uzorkovane 7-og i 21-og dana ogleda (P>0,05). Rezultati istraživanja pokazali su da je koncentracija HDL-C u plazmi PW bila u pozitivnoj korelaciji sa sadržajem alfa-linolenskih (ALA) masnih kiselina nakon 7-og dana (r = 0,94, P <0,0001) i 21-og dana ogleda (r = 0,89, P <0,0001), dok je 21-og dana ispitivana mešavina uticala na sadržaj T-Chol (r = 0,52, P = 0,08) i TG (r = 0,54, P = 0,07). Fekalni skor (FS) kod PW je redukovan 7-og dana (P> 0,05), a 21-og dana je uočena tendencija smanjenja FS kao posledice dodavanja eksperimentalne smeše u ishrani prasadi (P = 0,07). Testirana smeša za ishranu prasadi je pozitivno je uticala na populacije fekalnih mikroorganizama i to smanjenjem Staphylococcus spp. (P= 0,001), E. coli (P <0,0001) i gljivica (P = 0,004), a takođe je uočena tendencija povećanja vrsta Lactobacillus spp. (P = 0,08). U zaključku, ispitivana smeša može biti alternativa za delimičnu zamenu SBM u ishrani prasadi zbog pozitivnog biohemijskog odgovora i dobrog uticaja na zdravlje creva.
Abstract
The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on some plasma parameters and intestinal health in weaned piglets was investigated. Forty piglets (Topigs hybrid; body weight BW=8.02±0.82 kg), age 30±3 days, were divided into 2 groups and fed 2 diets: control (C, based on corn-triticale-soybean meal (SBM)) and experimental (ELS:WM, where the ELS:WM mixture 8:1 partially replaces SBM). Blood samples were collected at 7 and 21 days postweaning (PW). A chemistry analyzer was used to determine the plasma lipid (total cholesterol, T-Chol; HDL-cholesterol, HDL-C; triglycerides, TG), mineral and enzymatic profile. Microbial populations from fecal samples were determined by counting the colonies obtained on selected media. There was no effect of dietary mixture inclusion on plasma parameters at 7 and 21 days PW (P>0.05). The plasma HDL-C concentration was positively correlated with the alpha-linolenic (ALA) fatty acids content of diet at days 7 (r=0.94, P<0.0001) and 21 PW (r=0.89, P<0.0001), while at day 21 PW the T-Chol (r=0.52, P=0.08) and TG (r=0.54, P=0.07) tended to be influenced by the dietary treatment. Fecal score (FS) decreased at day 7 PW (P>0.05), and at day 21 PW a tendency to decrease FS as a response to dietary mixture addition was observed (P=0.07). The tested dietary mixture positively affected the microbial fecal populations by decreasing the Staphylococcus spp. (P=0.001), E. coli (P<0.0001) and fungi (P=0.004), also tended to increase the Lactobacillus spp. (P=0.08). In conclusion, the dietary mixture could be an alternative to partially replace SBM in piglet's diet due to positive biochemical response and intestinal health.
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