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Food and Feed Research
2020, vol. 47, br. 1, str. 43-53
Brza spektrofotometrijska metoda za određivanje reaktivnih supstanci tiobarbiturne kiseline (TBARS) u hrani za kalifornijske pastrmke
Rakita Slađana M.a ORCID, Čolović Dušica S.a, Levart Alenka P.b, Banjac Vojislav V.a, Čolović Radmilo R.a ORCID, Dragojlović Danka M.a, Đuragić Olivera M.a ORCID
aInstitut za prehrambene tehnologije, Novi Sad, Srbija
bUniverzitet u Ljubljani, Biotehnički fakultet, Domžale, Slovenija

e-adresa: sladjana.rakita@fins.uns.ac.rs
Projekat:
Istraživanje savremenih biotehnoloških postupaka u proizvodnji hrane za životinje u cilju povećanja konkurentnosti, kvaliteta i bezbednosti hrane (MPNTR - 46012)

Ključne reči: hrana za ribe; reaktivne supstance tiobarbiturne kiseline; lipidna peroksidacija; validacija metode
Sažetak
Oksidativne promene u hrani za ribe nastale tokom skladištenja predstavljaju veliki problem u ishrani riba. Ovo istraživanje ima za cilj da predstavi brzu i jednostavnu metodu za određivanje reaktivnih supstanci tiobarbiturnekiseline (TBARS) bez hidrolize u komercijalnoj hrani za kalifornijeske pastrmke, kao metodu za određivanje lipidne peroksidacije. Metoda je validovana i ispitane su sledeće karakteristike: linearnost (opseg), granica detekcije, granica kvantifikacije, preciznost (ponovljivost i unutar laboratorijska reproduktivnost), tačnost i stabilnost metode (Xi Rkontrolne karte). Prikazani rezultati ukazuju da je TBARS metoda linearna u opsegu 1.11-13.33 nmol/mL i precizna u uslovima ponovljivosti i unutar laboratorijske reproduktivnosti. S obzirom na malu koncentraciju analita u uzorku metoda je pokazala zadovoljavajuću tačnost. Takođe, metoda je stabilna u pogledu promenljivosti srednjih vrednosti merenja i razlika između merenja. Rezultati dobijeni ovim istraživanjem su ukazali da se ova metoda može uspešno primeniti za određivanje TBARS-a u hrani za kalifornijske pastrmke tokom rutinske kontrole kvaliteta u akvakulturi kada je potrebno analizirati veliki broj uzoraka za kratko vreme.
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O članku

jezik rada: engleski
vrsta rada: originalan članak
DOI: 10.5937/FFR2001043R
objavljen u SCIndeksu: 04.07.2020.
metod recenzije: jednostruko anoniman
Creative Commons License 4.0

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