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2011, vol. 65, br. 6, str. 697-706
In vitro ispitivanje potencijalne toksičnosti oralno tkivnih kondicionera
aUniverzitet u Nišu, Medicinski fakultet, Stomatološka klinika, Srbija
bUniverzitet u Nišu, Tehnološki fakultet, Leskovac, Srbija
cUniverzitet u Nišu, Medicinski fakultet, Katedra Stomatološka protetika, Srbija
dUniverzitet u Nišu, Medicinski fakultet, Institut za biologiju sa humanom genetikom, Srbija

e-adresakrunic@ni.ac.rs
Ključne reči: citotoksičnost; oralno tkivni kondicioneri; MTT test; HPLC
Sažetak
Oralno tkivni kondicioneri predstavljaju materijale koji se povremeno aplikuju na gingivalnu površinu pločaste zubne proteze, a radi eliminacije mehaničkih iritacija i ozdravljenja oštećene i inflamirane sluzokože. Po hemijskom sastavu mogu se podeliti na metakrilatne i silikonske kondicionere. Cilj istraživanja bio je ispitivanje potencijalne toksičnosti jednodnevnih, sedmnodnevnih i tridesetodnevnih ekstrakata oralno tkivnih kondicionera različitih koncentracija. U istraživanju su korišćeni ekstrakti dva silikonska i četiri metakrilatna materijala različitih efektivnih koncentracija (5, 12,5, 25 i 50%). Količina toksičnih supstanci i dinamika njihovog oslobađanja iz metakrilatnih kondicionera praćeni su tečnom hromatografijom pod visokim pritiskom (HPLC). Ova analiza nije mogla da se primeni na silikonske kondicionere jer se oni sastoje od polimernih i neorganskih supstanci. Ocena proliferativnosti ćelijske kulture, kao parametara citotoksičnosti, vršena je Mosmann-ovim MTT testom, koji se zasniva na redukciji žute tetrazolijumove soli (3-(4,5-dimetiltiazolil-2)-2,5-difeniltetrazolium bromid - MTT) mitohondrijalnom sukcinat dehidrogenazom metabolički aktivnih ćelija, pri čemu nastaje plavi formazan. Sa porastom dužine ekstrakcionog perioda raste i količina oslobođenih potencijalno toksičnih supstanci iz uzoraka metakrilatnih kondicionera. Citotoksičnost ispitivanih materijala značajno raste sa porastom koncentracije ispitivanih ekstrakata, kao i sa povećanjem dužine trajanja ekstrakcionog perioda. S ciljem poboljšanja bioloških karakteristika pločastih zubnih proteza podloženih mekim materijalima preporučuje se njihovo potapanje u vodu dan pre predaje pacijentu. Silikonski kondicioneri su pokazali manju citotoksičnost u odnosu na metakrilatne kondicionere, što ih preporučuje za primenu u svakodnevnoj stomatološkoj praksi.
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O članku

jezik rada: srpski
vrsta rada: stručni članak
DOI: 10.2298/HEMIND110627056K
objavljen u SCIndeksu: 25.01.2012.

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