- citati u SCIndeksu: 0
- citati u CrossRef-u:0
- citati u Google Scholaru:[
 ]
- posete u poslednjih 30 dana:5
- preuzimanja u poslednjih 30 dana:3
|
|
|
2014, vol. 30, br. 3, str. 529-538
|
|
Genetička varijabilnost klijanaca crvene deteline u odnosu na soni stres
Genetic variability of red clover seedlings in relation to salt stress
Projekat: Primena novih biotehnoloških rešenja gajenja goveda, ovaca i koza u cilju dobijanja biološki vredne i zdravstveno bezbedne hrane (MPNTR - 31053)
Sažetak
Crvena detelina je vrlo osetljiva biljka na veću koncentarciju soli, posebno tokom klijanja i rane faze porasta klijanaca. Cilj ovog istraživanja bio je da se proceni uticaj različite zaslanjenosti (0, 50, 100, 150 i 200mM NaCl) na klijanje i rani porast klijanaca četiri sorte crvene deteline (Kolubara, K-32, K-17 i K-39). Test klijavosti sproveden je u laboratorijskim uslovima u sterilnim plastičnim posudama na filter papiru natopljenom sa 10 ml odgovarajuće koncentracije soli. Uočeno je da energija klijanja (EK), klijavost (K), neklijala i bolesna semena (NB), normalni klijanci (NK), dužina korena (DK), dužina hipokotila (DH), sveža (SvMK) i suva masa klijanaca (SuMK) i vigor indeks klijanaca (VIK) se značajno smanjuju sa povećanjem koncentracije NaCl u podlogama za naklijavanje. Ispitivane sorte imale su različitu toleranciju na soni stres u fazi klijanaca. Generalno, proučavane sorte su veoma osetljive na veću koncentraciju soli, posebno K-32 koja je imala najniže vrednosti za EK, K, NK i VIK, kao i najveći broj NB. Sorta K-17 se pokazala kao sorta koja najbolje toleriše soni stres jer su vrednosti za EK, K, NK, DK i VIK bile najviše. Testiranje sorti crvene deteline u ranom porastu klijanaca na podlogama za naklijavanje sa različitom koncentracijom NaCl može pomoći u indentifikaciji i izboru sorti za gajenje na zaslanjenim zemljištima.
Abstract
Red clover is highly salt-sensitive plant, especially during germination and early seedling growth stages. The aim of this investigation was to estimate the effects of different saline conditions (0, 50, 100, 150, and 200mM NaCl) on germination and early seedling growth in four red clover varieties (Kolubara, K-32, K-17 and K-39). Germination test was conducted in the laboratory conditions using sterile plastic vessels on filter paper moistened with 10ml of the appropriate salt test solutions. It was observed that the germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium. The tested varieties of red clover showed different NaCl tolerance at the seedling stage. Generally, studied red clover varieties are very sensitive to salt, especially K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS. Variety K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest. Testing of varieties of red clover in the early seedling growth at different concentrations of NaCl in the growing medium could be helpful in the identification and selection of varieties for cultivation on saline soils.
|
|
|
|
Reference
|
|
 
|
Asci, O.O. (2011) Salt tolerance in red clover (Trifolium pratense L.) seedlings. African Journal of Biotechnology, 10, 44, 8774-8781
|
|
|
Asgharipour, M.R., Rafiei, M. (2011) Effect of salinity on germination and seedling growth of lentils. Australian Journal of Basic and Applied Sciences, 5, 11, 2002-2004
|
|
|
Ashraf, M., Mcneilly, T., Bradshaw, A.D. (1987) Selection and heritability of tolerance to sodium chloride in four forage species. Crop Sci, 227, 232-234
|
|
|
Ates, E., Tekeli, A.S. (2007) Salinity tolerance of Persian clover (Trifolium resupinatum var. majus Boiss) lines at germination and seedling stage. World Journal of Agricultural Sciences, 3, 71-79
|
|
|
Atis, I., Atak, M., Can, E., Mavi, K. (2011) Seed coat color effects on seed quality and salt tolerance of red clover (Trifolium pratense). International Journal of Agriculture and Biology, 13, 363-368
|
|
|
Can, E., Arslan, M., Sener, O., Daghan, H. (2013) Response of strawberry clover (Trifolium fragiferum L.) to salinity stress. Research on Crops, 14, 576-584
|
|
|
Dornbos, D.L. (1995) Seed vigour. u: Basra A.S. [ur.] Seed quality, New York: Food Products Press, 45-80
|
|
1
|
Elçi, S. (2005) Legume and graminae feed plants. Ankara: Turkish Ministry of Agriculture and Rural Affairs
|
|
1
|
Ferguson, J. (1995) An introduction to seed vigour testing. u: Van de Vanter H. A. [ur.] ISTA Seed Vigor Testing Seminar, ISTA, 1-10
|
|
|
Ghaderi-Far, F., Gherekhloo, J., Alimagham, M. (2010) Influence of environmental factors on seed germination and seedling emergence of yellow sweet clover (Melilotus officinalis). Planta Daninha, 28(3): 463-469
|
|
|
Gravandi, S. (2013) The examination of different NaCl concentrations on germination, radicle length and plumule length on three cultivars of clover. Annals of Biological Research, 4, 5, 200-203
|
|
|
Guan, B., Zhou, D., Zhang, H., Tian, Y., Japhet, W., Wang, P. (2009) Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature. Journal of Arid Environments, 73(1): 135-138
|
|
11
|
ISTA (2008) International rules for seed testing. Bassersdorf, Switzerland: International Seed Testing Association
|
|
11
|
ISTA (2009) International rules for seed testing. Bassersdorf, Switzerland: International Seed Testing Association
|
|
11
|
ISTA (1999) International rules for seed testing. International Seed Testing Association, Seed Sci. Technol, p 27
|
|
|
Kara, B., Uysal, N. (2010) Effect of different salinity (NaCl) concentrations on the first development stages of root and shoot organs of wheat. Anadolu J. Agric. Sci., 25(1); 37-43
|
|
|
Khan, M., GUL, B., Weber, D.J. (2002) Effect of temperature and salinity on the germination of Sarcobatus vermiculatus. Biologia Plantarum, 45(1): 133
|
|
|
Lianes, A., Reinoso, H., Luna, V. (2005) Germination and early growth of Prosopis strombulifera seedlings in different saline solutions. World Journal of Agricultural Sciences, 1, 2, 120-128
|
|
2 
|
Ličina, V., Nešić, L., Belić, M., Hadžić, V., Sekulić, P., Vasin, J., Ninkov, J. (2011) The soils of Serbia and their degradation. Ratarstvo i povrtarstvo, vol. 48, br. 2, str. 285-290
|
|
1
|
Machado, N.N.B., Saturnino, S.M., Bomfim, D.C., Custodio, C.C. (2004) Water stress induced by Mannitol and Sodium chloride in Soybean cultivars. Braz. Arch. Biol. Technol, 47, str. 521-529
|
|
|
Majidi, M.M., Jazayeri, M.R., Mohammadinejad, G. (2010) Effect of salt stress on germination characters and some ions accumulation of sainfoin (Onobrychis viciifolia Scop.) genotypes. Iranian Journal of Rangelands and Forests Plant Breeding Research, 17, 2, 256-269
|
|
1
|
Mandic, V., Krnjaja, V.S., Tomic, Z., Bijelic, Z., Zujovic, M., Simic, A.S., Prodanovic, S. (2012) Genotype, seed age and pH impacts on germination of alfalfa. Romanian biotechnological letters, vol. 17, br. 2, str. 7205-7211
|
|
1
|
Mandić, V., Tomić, Z., Krnjaja, V., Bijelić, Z., Žujović, M., Simić, A., Prodanović, S. (2011) Effect of acid stress on germination and early seedling growth of red clover. Biotechnology in Animal Husbandry, vol. 27, br. 3, str. 1295-1303
|
|
|
Munns, R., Husain, S., Rivelli, A.R., James, R.A., Condon, A., Lindsay, M.P., Lagudah, E.S., Schachtman, D.P., Hare, R.A. (2002) Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant and Soil, 247(1): 93-105
|
|
|
Nichols, P.G.H., Malik, A.I., Stockdale, M., Colmer, T.D. (2009) Salt tolerance and avoidance mechanisms at germination of annual pasture legumes: importance for adaptation to saline environments. Plant and Soil, 315(1-2): 241-255
|
|
2
|
Rogers, M.E., Noble, C.L., Halloran, G.M., Nicolas, M.E. (1995) The effects of NaCl on germination and early seedling growth of white clover (Trifolium repens L) populations selected for high and low salinity tolerance. Seed Science and Technology, 23, 277-287
|
|
|
Saberi, M., Davari, A., Pouzesh, H., Shahriari, A. (2013) Effect of different levels of salinity and temperature on seeds germination characteristics of two range species under laboratory condition. International Journal of Agriculture and Crop Sciences, 5, 14, 1553-1559
|
|
|
Shereen, A., Ansari, R. (2001) Salt Tolerance in Soybean (Glycine max L.): Effect on Growth and Water Relations. Pakistan Journal of Biological Sciences, 4(10): 1212-1214
|
|
1
|
Vieira, R.D., Carvalho, N.M. (1994) Teste de vigor em sementes. Jaboticabal: Funep/Unesp
|
|
|
Zhanwu, G., Hui, Z., Jicai, G., Chunwu, Y., Chunsheng, M., Deli, W. (2011) Germination responses of Alfalfa (Medicago sativa L.) seeds to various salt-alkaline mixed stress. African Journal of Agricultural Research, 6, 16, 3793-3803
|
|
|
|
|