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Optimizacija rasta Saccharomyces cerevisiae (PTCC 24860) na pretretiranoj melasi za proizvodnju etanola primenom metode odzivne površine
aSchool of Chemical Engineering, Noushirvani University of Technology, Babol, Iran
bIslamic Azad University, Ayatollah Amoli Branch, Amol, Iran
Sažetak
Rast Saccharomyces cerevisiae (PTCC 24860) na pretretiranoj melasi iz šećerne repe je optimizovan primenom statističkog pristupa. U cilju oslobađanja svih monomernih šećera, melasa je tretirana razblaženom kiselinom. Uticaj parametara procesa, kao što su: koncentracija šećera, izvor azota, pH i vreme inkubacije na rast ćelija je statistički procenjen pomoću softvera Design Expert i aplikacijom centralnog kompozitnog dizajna (CCD) i metode odzivne površine. Optimalni uslovi gajenja su bili: pH 5,3, vreme inkubacije 24 h i sastav podloge od 35 g redukujućih šećera, 1,5 g NH4Cl i 1 g ekstrakta kvasca po litri podloge. Pri optimalnim uslovima rasta ćelija i vremenu inkubacije 12 h ostvarena je maksimalna proizvodnja etanola od 14,87 g/L.
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O članku

jezik rada: engleski
vrsta rada: naučni članak
DOI: 10.2298/CICEQ100201029S
objavljen u SCIndeksu: 13.07.2010.

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