2016, vol. 66, br. 2, str. 187-202
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Izuzimanje humanog DNK profila sa obrisa životinjske dlake
Gleaning a human DNA profile from trace swabs collected from animal hairs
aFaculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia bUniversity Department for Forensic Sciences, University of Split, Split, Croatia cForensic Science Centre 'Ivan Vučetić', General Police Directorate, Ministry of Interior, Zagreb, Croatia
e-adresa: gmrsic@mup.hr
Ključne reči: DNA profiling; sheep wool; dog hair; Chelex® 100; Qiagen EZ1® DNA Investigator Kit
Sažetak
Životinjske dlake su pogodna površina za zadržavanje tragova epitela kao forenzičkih uzoraka. Cilj ovog istraživanja je bio trostruki: procijeniti različite metode uzimanja uzoraka (vlažni i suvi obris) i ekstrakcije DNK (Chelex® 100 metoda iQiagenEZ1® DNA Investigator Kit), uporediti morfološke karakteristike vlakana dlake poreklom od dve vrste (pas, ovca) i njihov konačni učinak na uzimanje i obradu uzoraka. Preliminarni rezultati upućuju kako upotreba EZ1® DNA Investigator Kit-a rezultira kvalitetnijim profilom DNK donora. Rezultati različitih metoda uzimanja uzoraka pokazuju unutarvrsne razlike koje zahtevaju dalje istraživanje. Sposobnost zadržavanja i kasnije ekstrakcije DNK tragova je slična u obe ispitane vrste, uprkos značajnim morfološkim razlikama u njihovim dlačnim pokrivačima.
Abstract
Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.
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