2016, vol. 66, br. 2, str. 214-221
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Efekti glifosata na enzimsku aktivnost i glukozu u serumu kod pacova Rattus norvegicus
Effects of glyphosate on enzyme activity and serum glucose in rats Rattus norvegicus
aUniverzitet u Sarajevu, Prirodno-matematički fakultet, Federacija BiH bPublic Health Center 'Mustafa Šehović', Tuzla, Bosnia and Herzegovina
e-adresa: mfocak10@mail.com
Sažetak
Glifosat je pesticid koji utiče na mnoge parametre u krvi ukoliko se unese oralno ili subkutano. Ovaj pesticid izaziva snažne promene u metaboličkoj aktivnosti što se može meriti promenama aktivnosti organospecifičnih enzima, među kojima su najznačajniji aminotransferaze (AST i ALT), dok je glukoza indikator stresnog stanja i energetskog metabolizma nakon akutnog tretmana glifosatom. U ovom istraživanju, glifosat je apliciran subkutano, na svakih 24 časa u periodu od 15 dana. Doza glifosata korišćena u ovom tretmanu iznosila je 2.8 g/kg. Eksperimentalni pacovi su bili starosne dobi od 13 nedelja. Koncentracija serumske glukoze, aktivnost laktat dehidrogenaze i jetrenih transaminaza (AST i ALT) su analizirane kao indikatori promena koje su nastupile nakon tretmana glifosatom. Utvrđeno je da glifosat dovodi do statistički značajnog sniženja koncentracije serumske glukoze. Povećanje aktivnosti AST, ALT i LDH (p<0.05) u serumu je indikator oštećenja hepatocita i njihovog isplavljivanja dok promene LDH ukazuje na oštećenja drugih tkiva i organa.
Abstract
Glyphosate is a pesticide that influences many blood parameters if taken orally or subcutaneously. This pesticide causes important changes in the metabolic activity which can be measured by organospecific enzyme activity such as liver aminotransferases (AST and ALT), while glucose acts as a stress, energy and metabolism indicator after acute glyphosate exposure. In this research, glyphosate was applied subcutaneously to rats, administrated each 24 hours for a 15 days period. The concentration of the applied glyphosate was 2.8 g/kg. The experimental rats were 13 weeks old. The concentration of serum glucose, the activity of lactate dehydrogenase and liver transaminases (AST and ALT) were observed as indicators of metabolic changes after treatment. It was observed that glyphosate led to a statistically significant decrease of serum glucose level. Statistically significantly increased (p<0.05) AST, ALT and LDH activities are indicators of hepatocyte damage while LDH activity demonstrates damage of other tissues.
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