2016, vol. 66, br. 3, str. 294-303
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Efekti Lactobacillus plantarum LS/07 na aktivnosti enzima crevnih bakterija u preveniranju kancera, ateroskleroze i disbioze
Effect of Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities in the prevention of cancer, atherosclerosis and dysbiosis
Institute of Experimental Medicine, Medical Faculty, University of P. J. Šafárik, Košice, Slovak Republic
e-adresa: emilia.hijova@upjs.sk
Projekat: Project of the Agency of the Slovak Ministry of Education, Science, Research, and Sport of the Slovak Republic for the Structural Funds of the EU, no. ITMS 26220120058
Project of the Agency of the Slovak Ministry of Education, Science, Research, and Sport of the Slovak Republic for the Structural Funds of the EU, no. ITMS 26220220104
Project of the Agency of the Slovak Ministry of Education, Science, Research, and Sport of the Slovak Republic for the Structural Funds of the EU, no. ITMS 26220220152
Project VEGA 1/0279/13
Project VEGA 1/0309/16
Sažetak
Ispitivani su efekti probiotičkog soja Lactobacillus plantarum LS/07 na aktivnosti bakterijskih enzima - β-glukuronidaze (β-GLUCUR), β-galaktozidaze (β-GAL) i β-glukozidaze (β-GLU) u prevenciji kancera, ateroskleroze i disbioze. Sprague-Dawley muški pacovi su metodom slučajnog izbora, podeljeni u 12 eksperimentalnih grupa: C (kontrolna grupa), AT (grupa ateroskleroze), CC (karcinogena grupa), svaka grupa u kombinaciji samo sa antibioticima ili probioticima kao i svaka grupa sa dvostrukom kombinacijom antibiotika i probiotika. U kontrolnoj grupi, aktivnost β-GLUCUR nije se menjala tokom trajanja ogleda. Ishrana sa velikom količinom masti u AT grupi, uslovila je značajno povećanje aktivnosti β-GLUCUR (p<0,001) i β-GLU (p<0,01). Aplikacija azoksimetana u CC grupi, značajno je povećala aktivnost β-GLUCUR (p<0,01) ali je smanjila β-GLU (p<0,01). Svakodnevna aplikacija probiotika, individualno i u kombinaciji sa antibioticima, povećala je aktivnost β-GAL, kao i β-GLU, uz pozitivno smanjivanje nivoa β-GLUCUR. U kontrolnoj antibiotskoj grupi, β-GLUCUR aktivnost se značajno povećala (p<0,05) uz smanjenje β-GLU (p<0,01). Ovo može da bude posledica promene mikroflore u korist koliformnih bakterija. Rezultati pokazuju da postoji pozitivan efekat Lactobacillus plantarum LS/07, što nadalje ukazuje na moguću upotrebu ove bakterije u prevenciji oboljenja u humanoj i veterinarskoj medicini.
Abstract
The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group significantly increased the activity of β-glucuronidase (p<0.001) and β-glucosidase (p<0.01). Azoxymethane application in the carcinogenic group significantly increased β-glucuronidase (p<0.01), but reduced β-glucosidase (p<0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was significantly increased (p<0.05), and β-glucosidase decreased (p<0.01) which can be caused by a change of microflora in favor of coliform bacteria. These finding indicate the positive effects of probiotic Lactobacillus plantarum LS/07 which allows its use in disease prevention in human and veterinary medicine.
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