2016, vol. 66, br. 3, str. 373-391
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Uticaj različitih hemijskih oblika selena dodatog hrani koja sadrži karnozinsku kiselinu, riblje ulje i ulje repice na nastanak isparljivih masnih kiselina i metana u buragu, kao i profil masnih kiselina u sadržaju buraga i mišićima jagnjadi
The influence of different chemical forms of selenium added to the diet including carnosic acid, fish oil and rapeseed oil on the formation of volatile fatty acids and methane in the rumen, and fatty acid profiles in the rumen content and muscles of lambs
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jabłonna, Poland
e-adresa: m.czauderna@ifzz.pl
Projekat: Project of the National Science Centre (NCN): Grant No.2013/09/B/NZ9/00291
Project of the Kielanowski Institute of Animal Physiology and Nutrition, PAS, Jabłonna, Poland (Project No. II.1; 2015)
Sažetak
Jagnjad su raspoređena u 3 grupe od po 6 životinja. Tokom 35 dana jagnjad je hranjena obrocima koji sadrže 2% ulje repice, 1% riblje ulje i 0,1% karnozinske kiseline (kontrolna grupa) ili dve ogledne suplementirane sa 0,35 mg/kg Se u obliku seleniziranog kvasca (SeY) ili selenata. Mišići Musculus longissimus dorsi (MLD) i Musculus biceps femoris (MBF), tečnost iz buraga i mikroflora su uzeti od svake životinje. Dijetarna suplementacija sa SeY je efikasno stimulisala nakupljanje isparljivih lančanih masnih kiselina (VFAs), iso-razgrenatih lanaca VFAs, CO2 i CH4 u ruminalnoj tečnosti. Obrok suplementiran selenitom je najefikasnije snižavao vrednosti CO2, CH4 i VFAs, uključujući linearne lance VFAs, sa izuzetkom iso-razgranatih lanaca VFAs. Kod kontrole u mikrobioti bio je najviši sadržaj iso-SFAs i neparnih zasićenih masnih kiselina. Dijetarna suplementacija sa SeY je u ruminalnoj tečnosti efikasno snizila odnos sirćetne kiseline prema propionskoj. Suplementacija selenatom je poboljšala performanse jagnjadi time što je u buragu snizila koncentraciju CH4 i CO2 Dodavanje selenita smanjilo je biohidrogenizaciju C 18:0 u poređenju sa kontrolom. Selenat je u poređenju sa SeY efikasnije snizio zbirnu koncentraciju svih SFA (SSFAs) i svih masnih kiselina (SFAs) u MLD i MBF. SeY je efikasnije uticao na porast koncentracija SFA (SSFAs) i masnih kiselina (SFAs) u MLD i MBF. Selenat je bio efikasan u povećanju telesne mase jagnjadi.
Abstract
Lambs were divided into 3 groups of 6 animals each. For 35 days lambs were fed a diet including 2% rapeseed oil, 1% fish oil and 0.1% carnosic acid (the control group) or two experimental diets supplemented with 0.35 mg ∙ kg-1 Se as selenized-yeast (SeY) (the SeY diet) or selenate (the selenate diet). Muscles (Musculus longissimus dorsi (MLD) and Musculus biceps femoris (MBF)), ruminal fluids and microbiota were collected from each lamb. SeY supplementation most effectively stimulated the accumulation of straightchain volatile fatty acids (VFAs), iso-branched-chain VFAs, CO2 and CH4 in the ruminal fluid. The contents of CO2, CH4 and VFAs including straight-chain VFAs with the exception of iso-branched-chain VFAs were most effectively reduced by the selenite diet. The control diet most efficiently increased the concentration sums of odd-saturated fatty acids (odd-SFAs) and iso-SFAs in microbiota. The SeY diet most efficiently reduced acetic acid to propionic acid ratio in the ruminal fluid. The selenate diet improved animal performance by reducing ruminal concentrations of CH4 and CO2. The SeY diet and especially the selenate diet reduced the biohydrogenation to C18:0 when compared with the control diet. The selenate diet more efficiently reduced the concentration sums of all SFAs (SSFAs) and all fatty acids (SFAs) in MLD and MBF than the SeY diet, which most effectively increased the concentrations of SSFAs and SFAs in MLD and MBF. The selenate diet most effectively increased the body mass gain of lambs.
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