2016, vol. 66, br. 4, str. 489-496
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Atipamezol - atenuisana telazol/ksilazin - indukovana ekspresija c-fos u talamencefaličnom i cerebralnom korteksu pacova
Atipamezole attenuated telazol/xylazine-induced expression of c-fos in rat thalamencephal and cerebral cortex
aDepartment of Veterinary Science, Jilin Agriculture Science and Technology College, Jilin, China + Key Lab of Preventive Veterinary Medicine in Jilin Province, Jilin, China + Key Lab of Preventive Veterinary Medicine in Jilin Province, Jilin, China bCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China cDepartment of Veterinary Science, Jilin Agriculture Science and Technology College, Jilin, China
e-adresa: hbwang1940@163.com
Projekat: Project of the National Natural Science Foundation of China, No. 31302150
Project of the National Natural Science Foundation of China, No. 31272617
Project of the National Natural Science Foundation of China, No. 31572580
Ključne reči: atipamezole; telazol; xylazine; rat; c-fos; brain
Sažetak
Cilj studije je bio procena da li atipamezol može da ograniči telazol/ksilazin - indukovanu ekspresiju c-fosa u mozgu pacova. Pacovima je ubrizgana mešavina 13,81 mg/ kg telazola i 5,21 mg/kg ksilazina. Nakon 10 minuta aplikovano je 0,522 mg/kg atipemezola, a nakon poslednje injekcije je sat kasnije pacovima uklonjen talamencefalični i cerebralni korteks. Nivo Fos proteina je izmeren u moždanom tkivu metodom Western blot. Rezultati su pokazali da atipamezol atenuira telazol/ksilazol indukciju ekspresije c-fos u talamencefaličnom i cerebralnom korteksu. Rezultati ukazuju da je atipemezol sposoban da inhibira telazol/ksilazin indukovanu ekspresiju c-fosa u mozgu pacova i tako zaštiti nerve od oštećenja.
Abstract
The aim of this study was to assess whether atipamezole can restrain telazol/xylazine induced expression of c-fos in the rat brain. Rats were injected with a mixture of 13.81 mg/kg telazol and 5.21 mg/kg xylazine, following 10 min later 0.522 mg/kg atipamezole. Thereon, the thalamencephal and cerebral cortex were removed one hour after the last injection. The level of Fos protein was measured in the brain tissue by Westernblot. The results revealed that atipamezole attenuates telazol/xylazine induction of c-fos expression in the thalamencephal and cerebral cortex. The results indicated that atipamezole is able to inhibit telazol/xylazine-induced c-fos expression in the rat brain, thus protecting it from nerve damage.
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