2016, vol. 66, br. 4, str. 520-533
|
|
Molekularna karakterizacija ESBL-produkujućih Escherichia coli izolata iz zdravih goveda i ovaca
Molecular characterization of ESBL-producing Escherichia coli isolated from healthy cattle and sheep
aDepartment of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey bDepartment of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
e-adresa: pehlivanoglu@mehmetakif.edu.tr
Projekat: Project of the Scientific and Technological Research Council of Turkey, (TUBITAK project number, 112O 820)
Project of the Mehmet Akif Ersoy University, Scientific Research Projects Unit, Turkey, No. 0158KAYDEP-13
Sažetak
Cilj studije je bio karakterizacija Escherichia coli izolata koji proizvode ESBL, a koji su izolovani iz zdravih goveda i ovaca u Burdur proviniciji u Turskoj. Uzorci fecesa od ukupno 200 goveda i 200 ovaca su ispitivani pri čemu je 31 uzorak od goveda (15,5%) i 3 uzoraka od ovaca (1,5%), bilo pozitivno na ESBL-produkujuće E. coli. Upotrebljen je kombinovani klinički i laboratorijski standardni metod Instituta. Od svih ESBL klasa gena, koji su ustanovljeni PCR metodom, blaCTX-M tip je bio najčešći, a sledile su blaTEM i blaSHV familije. Izolati E. coli koji proizvode ESBL pokazali su ko-rezistanciju na veći broj različitih antibiotika uključujući aminoglikozide, fenikole, quinolone, inhibitore folatnog puta i tetracikline. Stepen rezistencije je bio veći kod izolata poreklom od goveda, u poređenju sa izolatima od ovaca. Filogenetsko grupisanje E. coli izolata ukazuje da je grupa A (naročito A1) bila dominantna (19/34, 55,9%), pri čemu su sledile grupe B1 (9/34, 26,5%) i D (6/34, 17,6%); nijedan izolat nije spadao u B2 grupu. Studija pokazuje i to da se ESBL-produkujući E. coli izolati, nalaze u intestinalnoj flori zdravih goveda i ovaca u Burdur pokrajini Turske. Ovo je prvi prikaz koji ukazuje na pojavu CTX-M tipa ESBL-produkujućih E. coli izolata iz ovaca na farmama u Turskoj.
Abstract
The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute's combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey.
|