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Acta herbologica
2012, vol. 21, br. 2, str. 69-77
jezik rada: srpski
vrsta rada: naučni članak
objavljeno: 18/01/2019
Testiranje korovske vrste Solanum nigrum L. na rezistentnost prema triazinskim herbicidima
aInstitut za kukuruz 'Zemun polje', Beograd-Zemun
bUniversity of Life Sciences, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
cAdnan Menderes University, Agricultural Faculty, Aydın, Turkey

Projekat

Razvoj integrisanih sistema upravljanja štetnim organizmima u biljnoj proizvodnji sa ciljem prevazilaženja rezistentnosti i unapređenja kvaliteta i bezbednosti hrane (MPNTR - 46008)

Sažetak

Rezistentni biotipovi korova se najčešće javljaju nakon višegodišnje primene herbicida. Nakon zabrane primene atrazina, 2006. godine, u upotrebu je uveden terbutilazin, herbicid iz grupe triazina sa istim mehnizmom delovanja - zaustavljanjem procesa fotosinteze putem blokade transporta elektrona u PS II. Cilj rada je utvrđivanje rezistentnosti vrste Solanum nigrum L. na PS II inhibitore. Semena S. nigrum su uzorkovana sa mesta na kojima nikada nisu primenjivani herbicidi (potencijalna S populacija) i sa mesta na kojima je atrazin primenjivan kontinuirano dugi niz godina (potencijalna R populacija). Herbicid na bazi terbutilazina (500 g l-1 a.m.), je primenjen kao komercijalna formulacija u količinama 187,5, 375, 750 i 1500 g a.m. po ha, u fazi 4 lista korova. Fluorescencija hlorofila je zatim merena 1 sat, 1 dan i 2 dana nakon tretiranja i izračunata je maksimalna kvantna efikasnost PS II (Fv/Fm). Nakon toga, PCR analiza je sprovedena korišćenjem specijalno dizajniranih prajmera za psbA gen. Biljke R populacije su bile tolerantnije na delovanje herbicida i razvile veću biomasu u odnosu na biljke S populacije.Vrednosti maksimalne kvantne efikasnosti (Fv/Fm) su, dan nakon primene terbutilazina u preporučenoj količini, bile veće kod biljaka R populacije (0,588) nego kod biljaka S populacije (0,405). Analiza sekvenci genoma nije pokazala da je došlo do promene u nukleotidu u specifičnom regionu psbA gena odgovornog za rezistentnost prema triazinskim herbicidima tako da nije potvrđeno da je osnova povećane tolerantnosti ili rezistentnosti vrste S. nigrum prema triazinskim herbicidima genetska modifikacija u mestu delovanja.

Ključne reči

Solanum nigrum; rezistentnost; triazini; fluorescencija; PCR analiza

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