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2021, vol. 149, br. 3-4, str. 136-141
Ekspresija fibronektina u pulpi zuba pacova intoksikovanih olovom sa ekperimentalno izazvanim dijabetesom melitusom
aUniverzitet u Banjoj Luci, Medicinski fakultet, Katedra za bolesti zuba, Republika Srpska, BiH
bUniverzitet u Banjoj Luci, Medicinski fakultet, Katedra za patologiju, Republika Srpska, BiH
cUniverzitet u Banjoj Luci, Medicinski fakultet, Katedra za ortopediju vilica, Republika Srpska, BiH
dUniverzitet u Beogradu, Stomatološki fakultet, Klinika za bolesti zuba i endodonciju

e-adresairena.radman78@gmail.com
Ključne reči: fibronektin; odontogeneza; dijabetes; olovo
Sažetak
Uvod/Cilj Izloženost organizma olovu predstavlja jedan od značajnijih faktora koji utiču na opšte zdravlje uključujući oralno zdravlje, te ima uticaj na nastanak gleđnih i dentinskih defekata. Cilj ovog rada je bio da se imunohistohemijskom analizom odredi ekspresija medijatora odontogeneze fibronektina u pulpi zuba pacova sa ekperimentalno izazvanim dijabetesom melitusom, posle izlaganja životinja olovu. Metode Istraživanje je sprovedeno na 42 pacova soja vistar. Intoksikacija adultnih pacova olovnim acetatom je urađena putem vode za piće ad libitum. Prvu eksperimentalnu grupu (Exp_14 ) činilo je 16 pacova koji su dobijali olovo u vodi 14 dana, drugu grupu (Exp_30) 16 pacova koji su dobijali olovo u vodi 30 dana u istoj koncentraciji (1500 ppm), dok je kontrolnu grupu činilo 10 zdravih pacova. Grupe pacova Exp_14 i Exp_30 su uvedene u dijabetes melitus pomoću rastvora aloksana, koji je aplikovan intraperitonealno. Patohistološkom i imunohistohemijskom analizom određivana je ekspresija fibronektina u pulpi, odontoblastima, predentinu i dentinu zuba eksperimentalnih životinja. Rezultati Visoka difuzna pozitivnost fibronektina u grupi Exp_14 je uočena u 63,6% slučajeva, u grupi Exp_30 u 24% slučajeva, dok je u kontrolnoj grupi uočena u 50% slučajeva. Nije uočena statistički značajna razlika u ekspresiji fibronektina između ispitivanih grupa. Zaključak Intoksikacija olovom putem vode za piće, u periodu od 14 i 30 dana, imala je uticaj na ekspresiju fibronektina u pulpi, odontoblastima, predentinu i dentinu zuba životinja sa ekperimentalno izazvanim dijabetesom melitusom.
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O članku

jezik rada: engleski
vrsta rada: originalan članak
DOI: 10.2298/SARH191022102K
primljen: 22.10.2019.
revidiran: 12.10.2020.
prihvaćen: 15.10.2020.
objavljen onlajn: 06.11.2020.
objavljen u SCIndeksu: 18.05.2021.

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