• citations in SCIndeks: [2]
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:8
  • full-text downloads in 30 days:2


article: 3 from 8  
Back back to result list
2015, vol. 72, iss. 10, pp. 899-905
Artificial saliva effect on toxic substances release from acrylic resins
aUniveristy of Niš, Faculty of Medicine, Department of Prosthetic Dentistry
bUniveristy of Niš, Faculty of Medicine, Institute of Biology and Human Genetics
cUniveristy of Niš, Faculty of Technology, Leskovac
dUniveristy of Niš, Faculty of Science
eUniveristy of Niš, Faculty of Medicine
Virtual human osteoarticular system and its application in preclinical and clinical practice (MESTD - 41017)

Background/Aim. Acrylic-based resins are intensively used in dentistry practice as restorative or denture-base materials. The purpose of this study was to analyze the surface structure of denture base resins and the amount of released potentially toxic substances (PTS) immediately upon polymerization and incubation in different types of artificial saliva. Methods. Storage of acrylic samples in two models of artificial saliva were performed in a water bath at the temperature of 37 ± 1°C. Analysis of the surface structure of samples was carried out using scanning electronic microscopy analysis immediately after polymerization and after the 30-day incubation. The amounts of PTS per day, week and month extracts were measured using high-pressure liquid chromatography. Results. Surface design and amount of PTS in acrylic materials were different and depended on the types and duration of polymerization. The surfaces of tested acrylates became flatter after immersing in solutions of artificial saliva. The degree of acrylic materials release was not dependent on the applied model of artificial saliva. Conclusion. In order to improve biological features of acrylic resin materials, it was recommended that dentures lined with soft or hard coldpolymerized acrylates should be kept at least 1 to 7 days in water before being given to a patient. So, as to reach high degree of biocompatibility preparation of prosthetic restorations from heat-polymerized acrylate was unnecessary.
Ali, A., Bates, J.F., Reynolds, A.J., Walker, D.M. (1986) The burning mouth sensation related to the wearing of acrylic dentures: an investigation. British Dental Journal, 161(12): 444-447
Babich, H., Zuckerbraun, H.L., Wurzburger, B.J., Rubin, Y.L., Borenfreund, E., Blau, L. (1996) Benzoyl peroxide cytotoxicity evaluated in vitro with the human keratinocyte cell line, RHEK-1. Toxicology, 106(1-3), 187-96
Baker, S., Brooks, S.C., Walker, D.M. (1988) The release of residual monomeric methyl methacrylate from acrylic appliances in the human mouth: An assay for monomer in saliva. J Dent Res, 67(10): 1295-9
Bartolini, J.A., Murchison, D.F., Wofford, D.T., Sarkar, N.K. (2000) Degree of conversion in denture base materials for varied polymerization techniques. J Oral Rehabil, 27: 488-493
Bettencourt, A.F., Neves, C.B., Almeida, M.S., Pinheiro, L.M., Oliveira, S.A., Lopes, L.P., Castro, M.F. (2010) Biodegradation of acrylic based resins: A review. Dental materials, 26(5): e171-80
Boeckler, A.F., Morton, D., Poser, S., Dette, K. (2008) Release of dibenzoyl peroxide from polymethyl methacrylate denture base resins: an in vitro evaluation. Dental materials, 24(12): 1602-7
Cimpan, M.R., Matre, R., Cressey, L.I., Tysnes, B., Lie, S.A., Gjertsen, B.T., Skaug, N. (2000) The effect of heat- and auto-polymerized denture base polymers on clonogenicity, apoptosis, and necrosis in fibroblasts: denture base polymers induce apoptosis and necrosis. Acta Odontol Scand, 58(5): 217-28
Hanks, C.T., Wataha, J.C., Sun, Z. (1996) In vitro models of biocompatibility: A review. Dent Mater, 12(3): 186-93
Hongo, T., Sato, K., Sato, A. (1994) Amounts of residual monomer leached from clinically used acrylic resin denture base. J Dent Mater, 13(23); 252-3 (Japanese)
International Organisation for Standardization (2009) International Standard ISO 10993-5: Biological Evaluation of Medical Devices - Part 5: Tests for in vitro cytotoxicity. Geneva
International Standards Organization (2008) Dentistry base polymers part 1: Denture base polymers. Geneva: ISO, ISO 20795-1
Jorge, J.H., Giampaolo, E.T., Machado, A.L., Vergani, C.E. (2003) Cytotoxicity of denture base acrylic resins: a literature review. Journal of Prosthetic Dentistry, 90(2): 190-193
Jorge, J.H., Giampaolo, E.T., Machado, A.L., Pavarina, A.C., Carlos, I.Z. (2007) Biocompatibility of denture base acrylic resins evaluated in culture of L929 cells: Effect of polymerisation cycle and post-polymerisation treatments. Gerodontology, 24(1): 52
Kalipçilar, B., Karaağaçlioğlu, L., Hasanreisoğlu, U. (1991) Evaluation of the level of residual monomer in acrylic denture base materials having different polymerization properties. Journal of oral rehabilitation, 18(5): 399-401
Koda, T., Tsuchiya, H., Yamauchi, M., Hoshino, Y., Takagi, N., Kawano, J. (1989) High-performance liquid chromatographic estimation of eluates from denture base polymers. Journal of Dentistry, 17(2): 84-9
Kostić, M., Najman, S., Kocić, J.S., Krunić, N., Ajduković, Z., Petrović, D., Anđelković, M. (2008) Effect of denture base resin extracts on HeLa cells growth in vitro. Hemijska industrija, vol. 62, br. 3, str. 217-222
Lindh, L. (2002) On the adsorption behaviour of saliva and purified salivary proteins at solid/liquid interfaces. Swed Dent J Suppl, 152: 1-57
Luckachan, G.E., Pillai, C.K.S. (2011) Biodegradable Polymers- A Review on Recent Trends and Emerging Perspectives. Journal of Polymers and the Environment, 19(3): 637-676
Mikai, M., Koike, M., Fujii, H. (2006) Quantitative analysis of allergenic ingredients in eluate extracted from used denture base resin. Journal of oral rehabilitation, 33(3): 216-20
Moharram, M.A., Nour, A.K.N., Hakeem, N. A., Abou-Table, Z.A., Badr, N.A. (1992) Effect of cross-linking agents on the molecular properties of denture base resins. Journal of Materials Science, 27(22): 6041-6046
Moztarzadeh, F., Keyanpour-Rad, M., Shabani, V. (2003) Synthesis of a Photocurable Dental Ionomer Cement Based on the Reaction of Acryloyl Chloride Coupling Compound with Acrylic/Itaconic Acids Copolymer. Iran Polym J, 12(3); 211-6
Ogle, R.E., Davis, E.L. (1998) Clinical wear study of three commercially available artificial tooth materials: thirty-six month results. Journal of prosthetic dentistry, 79(2): 145-51
Pavarina, A.C., Vergani, C.E., Machado, A.L., Giampaolo, E.T., Teraoka, M.T. (2003) The effect of disinfectant solutions on the hardness of acrylic resin denture teeth. Journal of oral rehabilitation, 30(7): 749-52
Preetha, A., Banarjee, R. (2005) Comparison of artificial saliva substitutes. Trends Biomater Artif Organs, 18(2); 178-86
Radford, D.R., Sweet, S.P., Challacombe, S.J., Walter, J.D. (1998) Adherence of Candida albicans to denture-base materials with different surface finishes. J Dent, 26(7): 577-83
Satyanarayana, D., Chatterji, P.R. (1995) Studies on the enzymatic and fungal degradation of cross-linked gelatin and its graft copolymers. Journal of Environmental Polymer Degradation, 3(3): 177-186
Schmalz, G. (1994) Use of cell cultures for toxicity testing of dental materials: Advantages and limitations. J Dent, 22 Suppl 2: S6-11
van Joost, Th., van Ulsen, J., van Loon, L.A.J. (1988) Contact allergy to denture materials in the burning mouth syndrome. Contact Dermatitis, 18(2): 97-99
Zissis, A.J., Polyzois, G.L., Yannikakis, S.A., Harrison, A. (2000) Roughness of denture materials: a comparative study. Int J Prosthodont, 13(2); 136-40


article language: English
document type: Original Scientific Paper
DOI: 10.2298/vsp140304070k
published in SCIndeks: 06/11/2015
peer review method: double-blind

Related records

Acta stomatologica Naissi (2014)
Examination of adherence of dental acrylic polymers in vivo
Kostić Milena, et al.

Acta medica Medianae (2012)
Application of direct contact test in evaluation of cytotoxicity of acrylic denture base resins
Kostić Milena, et al.

Vojnosanitetski pregled (2018)
Denture base resins biocompatibility testing in vivo
Kostić Milena, et al.

show all [22]