Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:2
  • preuzimanja u poslednjih 30 dana:0

Sadržaj

članak: 3 od 7  
Back povratak na rezultate
Vojnosanitetski pregled
2016, vol. 73, br. 3, str. 256-260
Prevalencija Panton-Valentin leukocidin gena u vanbolničkim meticilin-rezistentnim Staphylococcus aureus u Pomoravskom okrugu
Petrović-Jeremić Ljiljanaa, Kuljić-Kapulica Nadab, Ristanović Elizabetab ORCID, Jošić Draganac, Lepšanović Zoricad ORCID
aCenter for Clinical Microbiology, Public Health Institution, Ćuprija
bVojnomedicinska akademija, Institut za mikrobiologiju, Beograd, Srbija + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd, Srbija
cInstitut za zemljište, Beograd, Srbija
dVojnomedicinska akademija, Institut za epidemiologiju, Beograd, Srbija + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd, Srbija

e-adresa: ljiljanapetrovicjeremi @hotmail.com
Ključne reči: meticilin; rezistencija; staphylococcus aureus; infekcije; vanbolničke; polimeraza; reakcija stvaranja lanaca; panton-valentin leukocidin; srbija
Sažetak
Uvod/Cilj. Vanbolnički (community-associated - CA) meticilin rezistentni Staphybcoccus aureus (CA-MRSA) se brzo širi u opštoj populaciji, pa i među onima koji nisu bili izloženi riziku boravka u bolnici. Panton Valentin leukocidin (PVL), kodiran lukF-PV i lukF-PV genima, može biti ključni toksin odgovoran za virulenciju vanbolničkog MRSA. Cilj rada bio je detektovati gene koji kodiraju PVL u izolatima vanbolničkog MRSA u Pomoravskom okrugu. Metode. Prisustvo S. aureus rezistentnog na meticilin testirano je tokom dvogodišnjeg perioda, na 52 910 briseva grla i nosa poreklom od zdravih, radno sposobnih ljudi koji podležu sanitarnom nadzoru. Brisevi su zasejavani na krvni agar i inkubirani 24 h. Rezistencija na meticilin detektovana je disk difuzionim testom sa diskom cefoksitina i E-testom, a test aglutinacije primenjen je za dokazivanje penicillin-vezujućih proteina 2a (PVP2a). Za detekciju mecA gena PVL gena i tipizaciju SCCmec regiona primenjena je polymerase chain reaction (PCR) metoda. Rezultati. Stafilokokno kliconoštvo bilo je prisutno kod 1 363 (2,58%) ispitanih, a MRSA je potvrđen kod 50 izolata S. aureus (3,67%). PVL gen je otkriven u dva (4%) CA-MRSA izolata. Jedan od PVL-pozitivnih izolata sadržao je SCCmec region tipa IV, a drugi tipa V. Zaključak. Prevalenca MRSA kod zdravih kliconoša, kao i zastupljenost PVL gena bili su niski. Vrednosti dobijene u ovoj studiji, pokazuju da se naš region ne razlikuje značajno od drugih delova naše zemlje i drugih evropskih zemalja.
Reference

Archer, G.L. (1998) Staphylococcus aureus : A Well-Armed Pathogen. Clinical Infectious Diseases, 26(5): 1179-1181

Brown, M.L., o'Hara F. Patrick,, Close, N.M., Mera, R.M., Miller, L.A., Suaya, J.A., Amrine-Madsen, H. (2011) Prevalence and Sequence Variation of Panton-Valentine Leukocidin in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Strains in the United States. Journal of Clinical Microbiology, 50(1): 86-90

Carleton, Heather A., Diep, B., Charlebois, Edwin D., Sensabaugh, George F., Perdreau‐Remington, F. (2004) Community‐Adapted Methicillin‐Resistant Staphylococcus aureus (MRSA): Population Dynamics of an Expanding Community Reservoir of MRSA. Journal of Infectious Diseases, 190(10): 1730-1738

Centers for Disease Control and Prevention Community associated MRSA information for clinicians: Infection control topics. [cited 2005 February 3]. Available from: http://www.cdc.gov/ncidod/dhqp/ar_mrs_ca_clinicians.html$4

Cirkovic, I., Sorum, M., Radenkovic, D., Vlahovic, M.S., Larsen, A.R. (2012) National surveillance reveals findings of Panton-Valentine leukocidin positive meticillin-resistant Staphylococcus aureus in Serbia. Journal of Medical Microbiology, 62(Pt_2): 342-344

Clinical Laboratory Standards Institute (2013) Performance standards for antimicrobial susceptibility testing. CLSI document M02-A11. Wayne, PA. Clinical and Laboratory Standards Institute, Approved standard 11th ed

Ćirković, I., Đukić, S., Vuković, D., Stevanović, G., Švabić-Vlahović, M., Stepanović, S. (2013) Nasal carriage of methicillin-resistant Staphylococcus aureus among medical students of Belgrade University. Srpski arhiv za celokupno lekarstvo, vol. 141, br. 5-6, str. 349-353

den Heijer, C.D.J., van Bijnen, E.M., Paget, W.J., Pringle, M., Goossens, H., Bruggeman, C.A., Schellevis, F.G., Stobberingh, E.E. (2013) Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study. Lancet Infectious Diseases, 13(5): 409-415

Diep, B.A., Gill, S.R., Chang, R.F., Phan, T.H., Chen, J.H., Davidson, M.G., Lin, F., Lin, J., Carleton, H.A., Mongodin, E.F. (2006) Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Lancet, 367(9512): 731-739

Eiff, C.von, Becker, K., Machka, K., Stammer, H., Peters, G. (2001) Nasal Carriage as a Source of Staphylococcus aureus Bacteremia. New England Journal of Medicine, 344(1): 11-16

Gordon, Rachel J., Lowy, Franklin D. (2008) Pathogenesis of Methicillin‐Resistant Staphylococcus aureus Infection. Clinical Infectious Diseases, 46(S5): S350-S359

Gouveia, C., Friaes, A., Neves, C.M., Melo, J., Ramirey, C.M. (2013) MRSA and PVL positive Staphylococcus aureus are rarely found in community-acquired osteoarticular infections in children in Portugal: A country with high MRSA Prevalence. Online Int J Micr Res, 1(2): 20-4

Hultén, Kristina G., Kaplan, Sheldon L., Lamberth, Linda B., Slimp, K., Hammerman, Wendy A., Carrillo‐Marquez, M., Starke, Jeffrey R., Versalovic, J., Mason, Edward O. (2010) Hospital‐Acquired Staphylococcus aureus Infections at Texas Children’s Hospital, 2001-2007. Infection Control and Hospital Epidemiology, 31(2): 183-190

1

Jones, M.E., Mayfield, D.C., Thornsberry, C., Karlowsky, J.A., Sahm, D.F., Peterson, D. (2002) Prevalence of oxacillin resistance in Staphylococcus aureus among inpatients and outpatients in the United States during 2000. Antimicrob Agents Chemother, 46(9): 3104-5

Krziwanek, K., Luger, C., Sammer, B., Stumvoll, S., Stammler, M., Metz-Gercek, S., Mittermayer, H. (2007) PVL-positive MRSA in Austria. European Journal of Clinical Microbiology & Infectious Diseases, 26(12): 931-935

Lazarevic, V., Beaume, M., Corvaglia, A., Hernandez, D., Schrenzel, J., François, P. (2011) Epidemiology and virulence insights from MRSA and MSSA genome analysis. Future Microbiology, 6(5): 513-532

Lina, G., Piemont, Y., Godail-Gamot, F., Bes, M., Peter, M.-O., Gauduchon, V., Vandenesch, F., Etienne, J. (1999) Involvement of Panton-Valentine Leukocidin--Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia. Clinical Infectious Diseases, 29(5): 1128-1132

Milheirico, C., Oliveira, D.C., de Lencastre, H. (2007) Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 51(9): 3374-3377

1

Moran, G.J., Krishnadasan, A., Gorwitz, R.J., Fosheim, G.E., Mcdougal, L.K., Carey, R.B., i dr. (2006) Methicillin-resistant S. aureus infections among patients in the emergency department. New England Journal of Medicine, 355(7): 666

Obradović, B., Kovačević, L., Miloradović-Aćimović, M., Purtić-Kljajić, D., Relić, T. (2009) Zastupljenost meticil-rezistentnih sojeva Staphylococcus aureus-a u zdravoj populaciji u Beogradu. Zdravstvena zaštita, vol. 38, br. 2, str. 27-31

o'Brien F.G., Lim, T.T., Chong, F.N., Coombs, G.W., Enright, M.C., Robinson, D.A., Monk, A., Said-Salim, B., Kreiswirth, B.N., Grubb, W.B. (2004) Diversity among Community Isolates of Methicillin-Resistant Staphylococcus aureus in Australia. Journal of Clinical Microbiology, 42(7): 3185-3190

1

Oliveira, D.C., Lencastre, H. (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrobial agents and chemotherapy, 46(7): 2155-61

1

Paez, A., Skiest, D. (2008) Methicillin-resistant Staphylococcus aureus: From the hospital to the community. Current Infectious Disease Reports, 10(1): 14

Petrović-Jeremić, L.J. (2009) Sensitivity of methicillin-resistent Strephylococcus anreus in hospital and non-hospital settings to the ather groups of antibiotics. PONS, 16: 18-25

Shukla, S.K., Stemper, M.E., Ramaswamy, S.V., Conradt, J.M., Reich, R., Graviss, E.A., Reed, K.D. (2004) Molecular Characteristics of Nosocomial and Native American Community-Associated Methicillin-Resistant Staphylococcus aureus Clones from Rural Wisconsin. Journal of Clinical Microbiology, 42(8): 3752-3757

van de Velde, H. (1894) Etude sur le mécanisme de la virulence du staphylocoque pyogène. La Cellule, 10: 401-10

Vandenesch, F., Naimi, T., Enright, M.C., Lina, G., Nimmo, G.R., Heffernan, H., Liassine, N., Bes, M., Greenland, T., Reverdy, M. (2003) Community-Acquired Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Genes: Worldwide Emergence. Emerging Infectious Diseases, 9(8): 978-984

Wright, J. (1936) Staphylococcal leucocidin (neisser-wechsberg type) and antileucocidin. Lancet, 227(5879): 1002-1005

Zhang, K., McClure, J., Elsayed, S., Tan, J., Conly, John M. (2008) Coexistence of Panton‐Valentine Leukocidin-Positive and -Negative Community‐Associated Methicillin‐Resistant Staphylococcus aureus USA400 Sibling Strains in a Large Canadian Health‐Care Region. Journal of Infectious Diseases, 197(2): 195-204
   

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/VSP140715003P
objavljen u SCIndeksu: 05.03.2016.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

Povezani članci

Nema povezanih članaka