In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber

Zdravković, Jasmina; Ugrinović, Milan; Zdravković, Milan; Đorđević, Slaviša; Pavlović, Snežana; Jošić, Dragana

doi:10.2298/pif1503169z

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Pesticidi i fitomedicina

2015, vol. 30, br. 3, str. 169-178

In vitro i in vivo efekti Pseudomonas spp. i Bacillus sp. na Fusarium acuminatum, Botrytis cinerea i Aspergillus niger na krastavcu

Zdravković Jasmina^a, Ugrinović Milan^a, Zdravković Milan^a, Đorđević Slaviša^b, Pavlović Snežana^c, Jošić Dragana^c

^aInstitut za povrtarstvo, Smederevska Palanka
^bUniverzitet u Beogradu, Poljoprivredni fakultet
^cInstitut za zemljište, Beograd

e-adresa: jzdravkovic@institut-palanka.co.rs

Projekat:

Novi autohtoni izolati bakterija Lysobacter i Pseudomonas kao važan izvor metabolita korisnih za biotehnologiju, stimulaciju rasta biljaka i kontrolu bolesti bilja: od izolata do preparata (MPNTR - 46007)
Novi koncept oplemenjivanja sorti i hibrida povrća namenjenih održivim sistemima gajenja uz primenu biotehnoloških metoda (MPNTR - 31059)

Ključne reči: Rizobakterije; Pseudomonas spp.; Bacillus sp.; Rast biljaka; Bolesti biljaka; Krastavac

Sažetak

Krastavac (Cucumis sativus L) je važan predstavnik familije Cucurbitaceae, a proizvodnja zdravog rasada je nephodna za visoku produktivnost u plastenicima i na otvorenom polju. Da bi se umanjila primena pesticida i mineralnih đubriva i pri tome sačuvalo zemljište, ispitan je uticaj bakterija stimulatora biljnog rasta (PGPB) na rast biljaka i zaštitu od patogena. Primenjeni su sojevi Pseudomonas spp. sa različitim antimikoznim delovanjem i Bacillus sp. soj Q10 sa PGP aktivnošću. Praćena je antagonistička aktivnost Pseudomonas spp. na fitopatogene gljive izolovane sa biljaka krastavca: F. acuminatum, B. cinerea i A. niger. Testiran je uticaj prekonoćne kulture bakterija, supernatanta i termostabilnih antifungalnih faktora na rast ovih fitopatogena. Pseudomonas sp. K35 i K24 sojevi, koji pokazuju 70-80% inhibicije rasta gljiva bez obzira na primenjenu kulturu ili frakciju, efektivniji su od P. chlororaphis Q16 i Pseudomonas sp. K27. Sojevi Pseudomonas spp., koji su ispoljili visok stepen antagonizma, kombinovani su sa sojem Bacillus sp. Q10 i procenjen je uticaj na rast i zdravstveno stanje biljaka krastavca. U zavisnosti od primenjenog soja Pseudomonas sp., razlikovala se dinamika rasta krastavca. Tretman M3 - kombinacija Bacillus sp. Q10 i P. chlororaphis Q16 uticala je na početnu fazu porasta biljaka, dok je treatman M4 - kombinacija Bacillus sp. Q10 i Pseudomonas sp. K24, imala najviše uticaja na visinu biljke na kraju merenja. Uočene su značajne razlike za masu lista i masu biljke (M4) i masu lista (M5 sa sojem K35), dok sadržaj hlorofila i nivo NBI nisu imali značajne razlike kod svih ispitivanih kombinacija. Dobijeni rezultati ukazuju da je M3 pogodan za rane faze razvoja biljke, a kombinacije M4 i M5 pogodne su za zaštitu biljaka i u kasnijim fazama porasta biljaka. Potpuna ekspresija PGP svojstava za M4 i M5 može se utvrditi tek posle testiranja na siromašnim supstratima, koji će biti upoređeni sa rezultaima dobijenim na supstratima sa dovoljno hranljivih materija.

Abstract

Cucumber (Cucumis sativus L) is an important member of the Cucurbitaceae family. Production of healthy nursery is necessary for high-quality production of this crop in greenhouses and in fields. With the idea of minimizing the use of pesticides and mineral fertilizers to preserve soil quality, we investigated the effects of plant growth promoting bacteria (PGPB) on growth promotion and protection of cucumber plants from phytopathogenic fungi. The effects of Pseudomonas spp. strains with different antifungal activities and Bacillus sp. Q10 strain with PGP activity were tested on cucumber plants. Antagonistic activity of Pseudomonas spp. against the growth of several phytopathogenic fungi isolated from cucumber: F. acuminatum, B. cinerea and A. niger, was observed. The influences of overnight cultures, supernatants and heat-stable antifungal factors were tested on the phytopathogenic fungi in vitro. Pseudomonas sp. K35 and K24 strains were more effective than P. chlororaphis Q16 and Pseudomonas sp. K27, showing 70-80% of fungal growth inhibition regardless of culture or fraction applied. The good antagonists that belong to pseudomonads and Bacillus sp. Q10 strain were used as mixtures for estimation of plant growth and health promoting effects on cucumber plants. Growth dynamics differed depending on the applied strain of Pseudomonas sp. The M3 treatment (a mixture of Bacillus sp. Q10 and P. chlororaphis Q16) stimulated the initial phase of growth, while M4 (a mixture of Bacillus sp. Q10 and Pseudomonas sp. K24) resulted in the maximal height at the final measurement. Significant differences in leaf and plant weight (M4), and leaf weight (M5, containing K35 strain) were found after the treatments. No significant differences in chlorophyll and NBI level were observed in any of the tested combinations. The obtained results suggested that M3 was suitable for stimulation of the early phase of cucumber growth, while the mixtures M4 and M5 improved plant protection and stimulated the later phases of cucumber growth.

	Reference
	Azami-Sardooei, Z. (2011) Induction of resistance to Botrytis cinerea in tomato, bean and cucumber by Serratia plymuthica and plant activators. Gent, Belgium: Universiteit Gent, Faculteit Bio-Ingenieurswetenschappen, PhD Thesis
	Ben-Shalom, N., Ardi, R., Pinto, R., Aki, C., Fallik, E. (2003) Controlling gray mould caused by Botrytis cinerea in cucumber plants by means of chitosan. Crop Protection, 22(2): 285-290
	Cao, Y., Zhang, Z., Ling, N., Yuan, Y., Zheng, X., Shen, B., Shen, Q. (2011) Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils, 47(5): 495-506
	Couderchet, M. (2003) Benefits and problems of fungicide control of Botrytis cinerea in vineyards of Champagne. Vitis, 165-171; 42
	Djuric, S., Pavic, A., Jarak, M., Pavlovic, S., Starovic, M., Pivic, R., Josic, D. (2011) Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. Romanian Biotechnological Letters, 16(5), 6580-6590
	Dursun, A., Ekinci, M., Dönmez, F.M. (2010) Effects of foliar application of plant growth promoting bacterium on chemical contents, yield and growth of tomato (Lycopersicon esculentum 1.) and cucumber (Cucumis sativus 1.). Pakistan Journal of Botany, 42(5); 3349-3356
	Eifediyi, E.K., Remison, S.U. (2010) Growth and yield of cucumber (Cucumis sativus L.) as influenced by farmyard manure and inorganic fertilizer. Journal of Plant Breeding and Crop Science, 2(7); 216-220
	Ghazvini, N.S., Pazoki, A., Tajali, A. (2014) Study the effect of plant growth promoting rhizobacteria (PGPR) and humic acid for some savory (Satureja hortensis L.) plant physiological traits under drought stress. Bulletin of Environment, Pharmacology and Life Sciences, 3(3); 182-185
4	Gomez, K.A., Gomez, A.A. (1984) Statistical procedures for agricultural research. New York: John Wiley & Sons
	Hayat, R., Ali, S., Amara, U., Khalid, R., Ahmed, I. (2010) Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology, 60(4): 579-598
	Hussain, A., Hasnain, S. (2009) Cytokinin production by some bacteria: Its impact on cell division in cucumber cotyledons. African Journal of Microbiology Research, 3(11); 704-712
	Jarak, M., Mrkovački, N., Bjelić, D., Jošić, D., Hajnal-Jafari, T., Stamenov, D. (2012) Effects of plant growth promoting rhizobacteria on maize in greenhouse and field trial. African Journal of Microbiology Research, 6(27): 5683-5690
1	Josic, D., Delic, D., Rasulic, N., Stajkovic, O., Kuzmanovic, D., Stanojkovic, A., Pivic, R. (2012) Indigenous Pseudomonads from rhizosphere of maize grown on pseudogley soil in Serbia. Bulgarian Journal of Agricultural Science, 18(2); 197-206
3	Jošić, D., Pavlović, S., Starović, M., Stojanović, S., Stanojković-Sebić, A., Pivić, R. (2012) Biocontrol of Alternaria tenuissima originated from Ocimum basilicum L using indigenous Pseudomonas spp. strains. u: Proceedings of 7th CMAPSEEC, Subotica: AMAPSEEC & IMPR Josif Pančić, pp. 195-200
	Jošić, D., Ćirić, A., Soković, M., Stanojković-Sebić, A., Pivić, R., Lepšanović, Z., Glamočlija, J. (2015) Antifungal activities of indigenous plant growth promoting Pseudomonas spp. from alfalfa and clover rhizosphere. Frontiers in Life Science, 8(2): 131-138
	Jošić, D., Pivić, R., Miladinović, M., Starović, M., Pavlović, S., Đurić, S., Jarak, M. (2012) Antifungal activity and genetic diversity of selected Pseudomonas spp. from maize rhizosphere in Vojvodina. Genetika, vol. 44, br. 2, str. 377-388
6	Jošić, D., Protolipac, K., Starović, M., Stojanović, S., Pavlović, S., Miladinović, M., Radović, S. (2012) Phenazines producing pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. Archives of Biological Sciences, vol. 64, br. 4, str. 1495-1503
	Khanuchiya, S., Parabia, F.M., Patel, M., Patel, V., Patel, K., Gami, B. (2012) Effect of Pseudomonas fluorescence, P. aeruginosa and Bacillus subtilis as biocontrol agent for crop protection. Cibtech Journal of Microbiology, 1(1); 52-59
	Kishore, G.K., Pande, S., Podile, A.R. (2005) Biological control of collar rot disease with broad-spectrum antifungal bacteria associated with groundnut. Canadian Journal of Microbiology, 51(2): 123-132
	Kishore, G.K., Pande, S., Podile, A.R. (2006) Pseudomonas aeruginosa GSE 18 inhibits the cell wall degrading enzymes of Aspergillus niger and activates defence-related enzymes of groundnut in control of collar rot disease. Australasian Plant Pathology, 35(2): 259
	Kokalis-Burelle, N., Kloepper, J.W., Reddy, M.S. (2006) Plant growth-promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Applied Soil Ecology, 31(1-2): 91-100
	Ling, N., Xue, C., Huang, Q., Yang, X., Xu, Y., Shen, Q. (2010) Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt. BioControl, 55(5): 673-683
	Lukkania, N.J., Reddya, S.E.C. (2014) Evaluation of plant growth promoting attributes and biocontrol potential of native fluorescent Pseudomonas spp. against Aspergillus niger causing collar rot of ground nut. International Journal of Plant, Animal and Environmental Sciences, 4(4); 256-262
	Marín, P., Moretti, A., Ritieni, A., Jurado, M., Vázquez, C., González-Jaén, M.T. (2012) Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe. Food Microbiology, 31(2): 229-237
	Meyer, J.B., Lutz, M.P., Frapolli, M., Péchy-Tarr, M., Rochat, L., Keel, C., Maurhofer, M. (2010) Interplay between Wheat Cultivars, Biocontrol Pseudomonads, and Soil. Applied and Environmental Microbiology, 76(18): 6196-6204
	Moshabaki, I.F., Besharati, H. (2012) Effect of biofertilizers on yield and yield components of cucumber. Journal of Biology and Earth Sciences, 2(2), B83-B92
	Njegić, R., Žižić, M., Lovrić, M., Pavličić, D. (1991) Osnovi starističke analize. Beograd: Savremena administracija, III izdanje
	Parkunan, V., Ji, P. (2013) First Report of Leaf Spot on Onion Caused by Fusarium acuminatum in Georgia. Plant Disease, 97(10): 1384-1384
	Protolipac, K., Pavlović, S., Starović, M., Stojanović, S., Lepšanović, Z., Jošić, D. (2012) Antifungal activity of indigenous Pseudomonas isolates against Alternaria tenuissima isolated from Echinacea purpurea. u: Proceedings of 7th CMAPSEEC, Subotica: AMAPSEEC & IMPR Josif Pančić, pp. 187-191
	Raaijmakers, J.M., Paulitz, T.C., Steinberg, C., Alabouvette, C., Moënne-Loccoz, Y. (2008) The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant and Soil, 321(1-2): 341-361
	Sripontan, Y., Tan, C.W., Hung, M.H., Young, C.C., Hwang, S.Y. (2014) Effects of plant-growth-promoting microorganisms and fertilizers on growth of cabbage and tomato and Spodoptera litura performance. Journal of Asia-Pacific Entomology, 17(3): 587-593
	Starović, M., Josić, D., Pavlović, S., Drazić, S., Postić, D., Popović, T., Stojanović, S. (2013) The effectof IAA producing Bacillus sp. Q3 strain on marshmallow seed germination. Bulgarian Journal Agriculture Science, 19(3), 572-577
3	Stević, T., Pavlović, S., Stanković, S., Šavikin, K. (2012) Pathogenic microorganisms of medicinal herbal drugs. Archives of Biological Sciences, vol. 64, br. 1, str. 49-58
	Turan, M., Ekinci, M., Yildirim, E., Güneş, A., Karagöz, K., Kotan, R., Dursun, A. (2014) Plant growth-promoting rhizobacteria improved growth, nutrient, and hormone content of cabbage (Brassica oleracea) seedlings. Turkish Journal of Agriculture and Forestry, 38: 327-333
	Vuković, G.L., Pavlović, S.Đ., Ristić, M.S. (2009) Comparison of two sample preparation procedures for HPLC determination of ochratoxin A. Archives of Biological Sciences, vol. 61, br. 4, str. 639-644
	Wehner, T.C., Guner, N. (2004) Growth stage, flowering pattern, yield, and harvest date prediction of four types of cucumber tested at 10 planting dates. Acta Horticulturae, (637): 223-230
	Wing, N., Bryden, W.L., Lauren, D.R., Burges, W. (1993) Toxigenicity of Fusarium species and subspecies in section Gibbosum from different regions of Australia. Mycological Research, 97(12): 1441-1446
	Wolf, A., Fritze, A., Hagemann, M., Berg, G. (2002) Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. International Journal of Systematic and Evolutionary Microbiology, 52(6): 1937-1944
	Yolcu, H., Turan, M., Lithourgidis, A., Çakmakçi, R., Koç, A. (2011) Effects of plant growth-promoting rhizobacteria and manure on yield and quality characteristics of Italian ryegrass under semi arid conditions. Australian Journal of Crop Science, 5 (13): 1730-1736
	Zarrin, F., Saleemi, M., Zia, M., Sultan, T., Aslam, M., Rehman, R.U., Chaudhary, M.F. (2009) Antifungal activity of plant growth-promoting rhizobacteria isolates against Rhizoctonia solani in wheat. African Journal of Biotechnology, 8(2); 219-225

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/pif1503169z
objavljen u SCIndeksu: 02.01.2016.
metod recenzije: dvostruko anoniman

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