In this article is processed a method of collecting data on the available types and quantities of biomass in Serbia, intended for energy and for other purposes. The analysis was performed based on the changed proprietary relationships, economic transition, and variable technological and technical conditions in agricultural production, forestry, industrial wood processing, and collection of communal refuse. The obtained data were compared with the data collected one, two or three decades ago. The statistics and published data used in this article were collected from numerous references: Statistical yearbook of Serbia, papers published in journals, books, monographs, and studies. Biomass quantities were calculated based on following mass ratios: grain/stalks, fruits/cutoff branches, grapes/vine, cattle/manure, tree/wood residue, and number of residents/communal refuse. Investigation data were obtained from collected data of mass ratios in agricultural and forestry production, published in various references. On based precisely collected data in Serbia, 9.68 million tons of biomass residue are produced per year at farms, orchards and vineyards 600 thousand, 14 million tons from manure from cattle production, 771 thousand tons of residue from forestry, and 1.2 million tons from communal refuse. Total quantity of produced biomass in Vojvodina equals 26.4 million tons. Production the total amount of biomass has dropped for 20 to 30% compared to the last decade. In agreement to estimation based on the previous records of exploitation of biomass in Serbia, 30 to 40% of the total amount of biomass can be used for production of energy, electricity, and biofuel. The rest of this quantity can be used for fertilization, cattle bedspreads, vegetable production, metal polishing in metalworking industry, insulation in civil engineering, cosmetics industry, fodder production, etc.