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2016, vol. 53, iss. 1, pp. 1-8
A rapid test for detection of tribenuron-methyl resistance in sunflower
aInstitute of Field and Vegetable Crops, Novi Sad, Serbia
bInstitute of Lowland Forestry and Environment, Novi Sad, Serbia
cUniversity of Belgrade, Faculty of Agriculture, Serbia
Development of new varieties and production technology improvement of oil crops for different purposes (MESTD - 31025)

A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.
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article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov53-8412
published in SCIndeks: 06/05/2016
peer review method: double-blind
Creative Commons License 4.0

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