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2016, vol. 53, br. 1, str. 1-8
Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil
aNaučni institut za ratarstvo i povrtarstvo, Novi Sad, Srbija
bInstitut za nizijsko šumarstvo i životnu sredinu, Novi Sad, Srbija
cUniverzitet u Beogradu, Poljoprivredni fakultet, Srbija

e-adresadragana.miladinovic@ifvcns.ns.ac.rs
Projekat:
Razvoj novih sorti i poboljšanje tehnologija proizvodnje uljanih biljnih vrsta za različite namene (MPNTR - 31025)

Ključne reči: biološka proba; Helianthus annuus L.; herbicidi; selekcija; sulfoniluree; tolerantnost
Sažetak
Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov53-8412
objavljen u SCIndeksu: 06.05.2016.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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