Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:4
  • preuzimanja u poslednjih 30 dana:0

Sadržaj

članak: 3 od 6  
Back povratak na rezultate
2019, vol. 147, br. 9-10, str. 553-559
Uticaj ekspresije steroidnih receptora na angiogenezu, proliferaciju i apoptozu u miomima premenopauznih i postmenopauznih žena
aBar General Hospital, Bar, Montenegro
bClinical Centre of Montenegro, Clinic of Gynaecology and Obstetrics, Podgorica, Montenegro
cUniverzitet u Kragujevcu, Fakultet medicinskih nauka, Katedra za patološku fiziologiju
dUniverzitet u Kragujevcu, Fakultet medicinskih nauka, Katedra za ginekologiju sa akušerstvom
eBlood Transfusion Institute of Montenegro, Bar Organizational Unit, Bar, Montenegro

e-adresasmitrovic@medf.kg.ac.rs
Ključne reči: receptori za steroide; apoptoza; angiogeneza; premenopauza; postmenopauza
Sažetak
Uvod/Cilj Cilj ove studije je bio da se utvrdi uticaj ekspresije steroidnih receptora na markere angiogeneze, proliferacije i apoptoze ćelija mioma kod žena u premenopauzi i postmenopauzi. Metode Ovo je bila studija preseka, kliničko-eksperimentalna, retrospektivna, neintervencijska studija u polju istraživanja fundamentalnih mehanizama patogeneze bolesti, korišćenjem patohistoloških materijala iz postojeće arhive. Istraživanjem je obuhvaćeno 76 bolesnika sa dijagnostikovanim lejomioima uterusa, operativno lečenih na Klinici za ginekologiju i akušerstvo Kliničkog centra Kragujevac, Srbija. Prema menstrualnom statusu, formirane su dve eksperimentalne podgrupe. Prva grupa bile su žene u premenopauzi (n = 35; 46,2 ± 5,02 godina), a druga grupa bile su žene u postmenopauzi (n = 41; 60,25 ± 5,41 godina). Korišćeno je bojenje H&E za miom i miometrijum, kao i imunohistohemija za ERa, ERb, PRa, vaskularni endotelni faktor rasta, endoglin, Ki67 i caspase 3. Rezultati Progesteronski receptor je bio više izražen u miomu i miometrijumu u premenopauzi u poređenju sa miomom i miometrijumom žena u postmenopauzi. Ekspresija caspase-3 je statistički značajno povećana u grupi žena koje su u postmenopauzi u poređenju sa grupom žena koje su u premenopauzi. ERa i ERb nisu bili različiti imeđu grupa ni u uzorcima mioma ni miometrijuma. Zaključak Prema našim podacima, PRa je imao veći uticaj na apoptozu i rast ćelija nego receptori za estrogene. Pošto je PRa povećan kod žena u premenopauzi u miomu i miometrijumu, verovatno je ova ekspresija dovela do smanjenja ekspresije apoptotskog markera kod žena koje su u premenopauzi.
Reference
Awowole, I.O., Makinde, O.N., Badejoko, O.O., Omoniyi-Esan, G.O., Tijani, A.M., Ajenifuja, K.O. (2016) Clinical correlates of leiomyoma estrogen and progesterone receptors among Nigerian women. Int J Gynaecol Obstet, 135(3), 314-318
Borahay, M.A., Al-Hendy, A., Kilic, G.S., Boehning, D. (2015) Signaling pathways in leiomyoma: Understanding pathobiology and implications for therapy. Mol Med, 21, 242-256
Commandeur, A.E., Styer, A.K., Teixeira, J.M. (2015) Epidemiological and genetic clues for molecular mechanisms involved in uterine leiomyoma development and growth. Hum Reprod Update, 21(5), 593-615
Hague, S., Manek, S., Oehler, M.K., Mackenzie, I.Z., Bicknell, R., Rees, M.C. (2002) Tamoxifen induction of angiogenic factor expression in endometrium. Br J Cancer, 86(5), 761-767
Heidenhain, M. (1896) Noch einmal uber die darstellung der centralkorper durch eisnhamotoxylin: Nebst einigen allgemeinen bemerkungen uber die hamatoxylinfarben. Wiss Mikrosk, 13, 186-186
Javid, S., Ziamajidi, N., Foroughi, S., Abbasalipourkabir, R. (2017) Effects of tamoxifen-loaded solid lipid nanoparticles on the estrogen receptor-a (ER-a) and vascular endothelial growth factor-A (VEGF-A) genes expression in the endometrial tissue of ovariectomized female Sprague-Dawley rats. Int J Biol Macromol, 96, 706-712
Konarska, M., Wrona, A.N., Aleksandrovych, V., Bereza, T., Sajewicz, M., Gach-Kuniewicz, B. (2016) Angiogenesis and pro-angiogenic factors in uterine fibroids: Facts and myths. Folia Med Cracov, 56(2), 37-43
Laganà, A.S., Vergara, D., Favilli, A., Rosa, L.V.L., Tinelli, A., Gerli, S., et al. (2017) Epigenetic and genetic landscape of uterine leiomyomas: A current view over a common gynaecological disease. Arch Gynecol Obstet, 296(5), 855-867
Levy, G., Hill, M.J., Plowden, T.C., Catherino, W.H., Armstrong, A.Y. (2013) Biomarkers in uterine leiomyoma. Fertil Steril, 99(4), 1146-1152
Maruo, T., Ohara, N., Wang, J., Matsuo, H. (2004) Sex steroidal regulation of uterine leiomyoma growth and apoptosis. Hum Reprod Update, 10(3), 207-220
Patel, B., Elguero, S., Thakore, S., Dahoud, W., Bedaiwy, M., Mesiano, S. (2015) Role of nuclear progesterone receptor isoforms in uterine pathophysiology. Hum Reprod Update, 21(2), 155-173
Plewka, A., Plewka, D., Madej, P., Nowaczyk, G., Sieron-Stoltny, K., Jakubiec-Bartnik, B. (2011) Processes of apoptosis and cell proliferation in uterine myomas originating from reproductive and perimenopausal women. Folia Histochem Cytobiol, 49(3), 398-404
Plewka, D., Marczyński, J., Morek, M., Bogunia, E., Plewka, A. (2014) Receptors of hypothalamic-pituitary-ovarian-axis hormone in uterine myomas. Biomed Res Int, 2014, 521313
Reis, F.M., Petraglia, F., Taylor, R.N. (2013) Endometriosis: Hormone regulation and clinical consequences of chemotaxis and apoptosis. Hum Reprod Update, 19(4), 406-418
Saarelainen, S.K., Staff, S., Peltonen, N., Lehtimäki, T., Isola, J., Kujala, P.M. (2014) VEGF, and its receptors in predicting metastases in endometrial carcinoma. Tumour Biol, 35(5), 4651-4657
Sakaguchi, H., Fujimoto, J., Aoki, I., Tamaya, T. (2003) Expression of estrogen receptor Alpha and beta in myometrium of premenopausal and postmenopausal women. Steroids, 68(1), 11-19
Sen, N., Demirkan, N.C., Colakoglu, N., Duzcan, S.E. (2008) Are there any differences in the expression of hormonal receptors and proliferation markers between uterine and extrauterine leiomyomas?. Int J Surg Pathol, 16(1), 43-47
Sparić, R. (2014) Uterine myomas in pregnancy, childbirth and puerperium. Srpski arhiv za celokupno lekarstvo, vol. 142, br. 1-2, str. 118-124
Stewart, E.A., Cookson, C.L., Gandolfo, R.A., Schulze-Rath, R. (2017) Epidemiology of uterine fibroids: A systematic review. BJOG, 124(10), 1501-1512
Suvarna, K.S., Layton, C., Bancroft, J.D., ur. (2018) Bancroft's theory and practice of histological techniques. Churchill Livingstone/Elsevier Science
Tal, R., Segars, J.H. (2014) The role of angiogenic factors in fibroid pathogenesis: Potential implications for future therapy. Hum Reprod Update, 20(2), 194-216
Tinelli, A., Sparić, R., Kadija, S., Babović, I., Tinelli, R., Mynbaev, O.A. (2016) Myomas: Anatomy and related issues. Minerva Ginecol, 68(3), 261-273
Tsigkou, A., Reis, F.M., Lee, M.H., Jiang, B., Tosti, C., Centini, G. (2015) Increased progesterone receptor expression in uterine leiomyoma: Correlation with age, number of leiomyomas, and clinical symptoms. Fertil Steril, 104(1), 170-175.e1
Weidner, N., Semple, J.P., Welch, W.R., Folkman, J. (1991) Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N Engl J Med, 324(1), 1-8
Wu, X., Blanck, A., Olovsson, M., Möller, B., Favini, R., Lindblom, B. (2000) Apoptosis, cellular proliferation and expression of p53 in human uterine leiomyomas and myometrium during the menstrual cycle and after menopause. Acta Obstet Gynecol Scand, 79(5), 397-404
Yun, B.S., Seong, S.J., Cha, D.H., Kim, J.Y., Kim, M.L., Shim, J.Y. (2015) Changes in proliferating and apoptotic markers of leiomyoma following treatment with a selective progesterone receptor modulator or gonadotropin-releasing hormone agonist. Eur J Obstet Gynecol Reprod Biol, 191, 62-67
 

O članku

jezik rada: engleski
vrsta rada: originalan članak
DOI: 10.2298/SARH181030023M
objavljen u SCIndeksu: 16.11.2019.