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2013, vol. 141, iss. 3-4, pp. 155-162
Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
aUniveristy of Niš, Faculty of Medicine, Clinic of Stomatology
bUniversity of Belgrade, Faculty of Dental Medicine
cUniversity of Belgrade, Faculty of Dental Medicine, Clinic for Conservative Dentistry and Endodontics

emailalek.mitic@yahoo.co.uk
Keywords: infection; root canal; endopathogenic bacteria; PCR
Abstract
Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
References
Abdullah, M., Ng, Y.L., Gulabivala, K., Moles, D., Spratt, D.A. (2005) Susceptibilites of two Enterococcus faecalis phenotypes to root canal medications. J Endod, 31: 30-36
Ando, N., Hoshino, E. (1990) Predominant obligate anaerobes invading the deep layers of root canal dentin. Int Endod J, 23(1): 20-7
Baumgartner, J.C., Falkler, W.A. (1991) Bacteria in the apical 5 mm of infected root canals. Journal of endodontics, 17(8): 380-3
Calt, S., Serper, A. (2002) Time-dependent effects of EDTA on dentin structures. J Endod, 28(1): 17-9
Dahlen, G. (2002) Microbiology and treatment of dental abscesses and periodontal-endodontic lesions. Periodontol 2000, 28: 206-39
Evans, M., Davies, J.K., Sundqvist, G., Figdor, D. (2002) Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. International endodontic journal, 35(3): 221-8
Fabricius, L., Dahlén, G., Holm, S.E., Möller, A.J.R. (1982) Influence of combinations of oral bacteria on periapical tissues of monkeys. European Journal of Oral Sciences, 90(3): 200-206
Giuliana, G., Ammatuna, P., Pizza, G., Capone, F., D`Angelo, M. (1997) Occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings. Journal of Clinical Periodontology, 24(7): 478-485
Haapasalo, M., Endal, U., Zandi, H., Coil, J.M. (2005) Eradication of endodontic infection by instrumentation and irrigation solutions. Endodontic Topics, 10(1): 77-102
Heid, C.A., Stevens, J., Livak, K.J., Williams, P.M. (1996) Real time quantitative PCR. Genome Res, 6, 986-994
Horiba, N., Maekawa, Y., Matsumoto, T., Nakamura, H. (1990) A study of the distribution of endotoxin in the dentinal wall of infected root canals. Journal of endodontics, 16(7): 331-4
Love, R.M. (2001) Enterococcus faecalis - a mechanism for its role in endodontic failure. International Endodontic Journal, 34(5): 399-405
Mitić, A., Mitić, N., Živković, S., Tošić, G., Savić, V., Dačić, S., Stojanović, M. (2009) Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja. Srpski arhiv za celokupno lekarstvo, vol. 137, br. 9-10, str. 482-489
Molander, A., Reit, C., Dahlen, G., Kvist, T. (1998) Microbiological status of root-filled teeth with apical periodontitis. Int Endod J, 31(1): 1-7
Möller, A.J. (1966) Microbiological examination of root canals and periapical tissues of human teeth: Methodological studies. Goteborg: Akademiforlaget
Moore, W.E., Moore, L.H., Ranney, R.R., Smibert, R.M., Burmeister, J.A., Schenkein, H.A. (1991) The microflora of periodontal sites showing active destructive progression. J Clin Periodontol, 18(10): 729-39
Orstavik, D., Haapasalo, M. (1990) Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol, 6(4): 142-9
Perez, F., Rochd, T., Lodter, J.P., Calas, P., Michel, G. (1993) In vitro study of the penetration of three bacterial strains into root dentine. Oral Surg Oral Med Oral Pathol, 76(1): 97-103
Salvatorelli, G., de Lorenzi, S. (2002) Biofilms stato dellarte. Linternista, 10(4), str. 222-31
Shabahang, S., Pouresmail, M., Torabinejad, M. (2003) In vitro antimicrobial efficacy of MTAD and sodium hypochlorite. J Endod, 29(7): 450-2
Siqueira, J.F., de Uzeda, M. (1996) Disinfection by calcium hydroxide pastes of dentinal tubules infected with two obligate and one facultative anaerobic bacteria. Journal of endodontics, 22(12): 674-6
Siqueira, J.F., Rôças, L.N. (2003) Bacteroides forsythus in primary endodontic infections as detected by nested PCR. J Endod, 29: 390-393
Siqueira, J.F., Rôças, I.N. (2003) PCR-based identification of Treponema maltophilum, T. amylovorum, T. medium, and T. lecithinolyticum in primary root canal infections. Archives of Oral Biology, 48(7): 495-502
Siqueira, J.F., Rôças, I.N., Souto, R., de Uzeda, M., Colombo, A.P. (2002) Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections. Journal of endodontics, 28(3): 168-72
Siren, E.K., Haapasalo, M.P.P., Ranta, K., Salmi, P., Kerosuo, E.N.J. (1997) Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. International Endodontic Journal, 30(2): 91-95
Spratt, D.A., Pratten, J., Wilson, M., Gulabivala, K. (2001) An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. International Endodontic Journal, 34(4): 300-307
Sundqvist, G. (1992) Associations between microbial species in dental root canal infections. Oral Microbiol Immunol, 7(5): 257-62
Sundqvist, G., Figdor, D. (2003) Life as an endodontic pathogen. Ecological differences between the untreated and root-filled root canals. Endodontic Topics, 6(1): 3-28
Torabinejad, M., Ung, B., Kettering, J.D. (1990) In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod, 16(12): 566-9
Torabinejad, M., Shabahang, S., Aprecio, R.M., Kettering, J.D. (2003) The antimicrobial effect of MTAD: An in vitro investigation. J Endod, 29(6): 400-3
 

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article language: Serbian
document type: Original Paper
DOI: 10.2298/SARH1304155M
published in SCIndeks: 13/11/2013

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