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2009, vol. 56, iss. 4, pp. 171-175
Mutation status of p53 gene in oral squamous cell carcinoma
aUniversity of Belgrade, Faculty of Dental Medicine
bUniversity of Belgrade, Faculty of Medicine, Institute of Biology and Human Genetics
cUniversity of Belgrade, Faculty of Dental Medicine, Department of Maxillofacial Surgery
Projekat Ministarstva nauke Republike Srbije, br. 1454042

Introduction. p53 gene is the most common tumor suppressor gene involved in pathogenesis oral squamous cell carcinoma (OSCC). Protein product of p53 gene contributes to cell cycle control and apoptosis. p53 gene mutations may lead to uncontrolled cell growth. The aim of this study was to determine the incidence of mutation in DNA-binding domain of p53 gene. Materials and Methods. In the 60 specimens, the presence of point mutation in exons 5, 6, 7 and 8 was detected using PCR-SSCP method. To confirm the presence of p53 mutation found by SSCP method, five samples were analyzed by sequencing of exon 5. Results. Point mutation affecting exons 5, 6, 7 and 8 were found in 60% of analyzed samples. A higher incidence of mutation was detected in exon 7 and 8 (60%), than in exon 5 and 6. Sequencing of exon 5, confirmed the presence of mutations revealed by SSCP method. Study of associations showed an increase of p53 mutations in poor differentiated and carcinoma of higher clinical stages. Conclusion. p53 gene is one of major factor in control of cell cycle and has important role in pathogenesis of oral squamous cell carcinoma.
Bosch, F.X., Ritter, D., Enders, C., Flechtenmacher, C., Abel, U., Dietz, A., Hergenhahn, M., Weidauer, H. (2004) Head and neck tumor sites differ in prevalence and spectrum of p53 alterations but these have limited prognostic value. Int J Cancer, 111(4): 530-8
Boyle, J.O., Hakim, J., Koch, W., van der Riet, P., Hruban, R.H., Roa, R.A., Correo, R., Eby, Y.J., Ruppert, J.M., Sidransky, D. (1993) The incidence of p53 mutations increases with progression of head and neck cancer. Cancer Res, 53(19): 4477-80
Cabelguenne, A., Blons, H., de Waziers, I., Carnot, F., Houllier, A.M., Soussi, T., Brasnu, D., Beaune, P., Laccourreye, O., Laurent, P. (2000) p53 alterations predict tumor response to neoadjuvant chemotherapy in head and neck squamous cell carcinoma: A prospective series. J Clin Oncol, 18(7): 1465-73
Chaves, A.C.M., Cherubini, K., Herter, N., Furian, R., Santos, D.S., Squier, C., Domann, F.E. (2004) Characterization of p53 gene mutations in a Brazilian population with oral squamous cell carcinomas. Int J Oncol, 24(2): 295-303
Denissenko, M.F., Pao, A., Tang, M., Pfeifer, G.P. (1996) Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53. Science, 274(5286): 430-2
Greenlee, R.T., Murray, T., Bolden, S., Wingo, P.A. (2000) Cancer statistics, 2000. CA Cancer J Clin, 50(1): 7-33
Gudkov, A.V., Komarova, E.A. (2003) The role of p53 in determining sensitivity to radiotherapy. Nat Rev Cancer, 3(2): 117-29
Kress, S., Sutter, C., Strickland, P.T., Mukhtar, H., Schweizer, J., Schwarz, M. (1992) Carcinogen-specific mutational pattern in the p53 gene in ultraviolet B radiation-induced squamous cell carcinomas of mouse skin. Cancer Res, 52(22): 6400-3
Nagler, R.M., Kerner, H., Laufer, D., Ben-Eliezer, S., Minkov, I., Ben-Itzhak, O. (2002) Squamous cell carcinoma of the tongue: The prevalence and prognostic roles of p53, Bcl-2, c-erbB-2 and apoptotic rate as related to clinical and pathological characteristics in a retrospective study. Cancer Lett, 186(2): 137-50
Regezi, J.A., Jordan, R.C. (2001) Oral cancer in the molecular age. J Calif Dent Assoc, 29(8): 578-84
Yao, L., Iwai, M., Furuta, I. (1999) Correlations of bcl-2 and p53 expression with the clinicopathological features in tongue squamous cell carcinomas. Oral oncology, Vol. 35, No. 1, pp. 56-62
Zhang, Y., Xiong, Y., Yarbrough, W.G. (1998) ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways. Cell, 92(6): 725-34


article language: English, Serbian
document type: Original Paper
DOI: 10.2298/SGS0904171P
published in SCIndeks: 26/01/2010