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2009, vol. 56, iss. 4, pp. 171-175
Mutation status of p53 gene in oral squamous cell carcinoma
aUniversity of Belgrade, Faculty of Dental Medicine
bUniversity of Belgrade, Faculty of Medicine, Institute of Biology and Human Genetics
cUniversity of Belgrade, Faculty of Dental Medicine, Department of Maxillofacial Surgery

emailbrankapo@verat.net
Project:
Projekat Ministarstva nauke Republike Srbije, br. 1454042

Abstract
Introduction. p53 gene is the most common tumor suppressor gene involved in pathogenesis oral squamous cell carcinoma (OSCC). Protein product of p53 gene contributes to cell cycle control and apoptosis. p53 gene mutations may lead to uncontrolled cell growth. The aim of this study was to determine the incidence of mutation in DNA-binding domain of p53 gene. Materials and Methods. In the 60 specimens, the presence of point mutation in exons 5, 6, 7 and 8 was detected using PCR-SSCP method. To confirm the presence of p53 mutation found by SSCP method, five samples were analyzed by sequencing of exon 5. Results. Point mutation affecting exons 5, 6, 7 and 8 were found in 60% of analyzed samples. A higher incidence of mutation was detected in exon 7 and 8 (60%), than in exon 5 and 6. Sequencing of exon 5, confirmed the presence of mutations revealed by SSCP method. Study of associations showed an increase of p53 mutations in poor differentiated and carcinoma of higher clinical stages. Conclusion. p53 gene is one of major factor in control of cell cycle and has important role in pathogenesis of oral squamous cell carcinoma.
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article language: English, Serbian
document type: Original Paper
DOI: 10.2298/SGS0904171P
published in SCIndeks: 26/01/2010