• citations in SCIndeks: [4]
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:8
  • full-text downloads in 30 days:3


article: 4 from 9  
Back back to result list
2008, vol. 136, iss. 9-10, pp. 476-480
Identification of periodontopathogen microorganisms by PCR technique
aClinical Centre Niš, Clinic of Children's Internal Diseases
bUniversity of Belgrade, Faculty of Dental Medicine
cUniversity of Belgrade, Faculty of Dental Medicine, Department of Periodontology and Oral Medicine
INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.
Beikler, T., Schnitzer, S., Abdeen, G., Ehmke, B., Eisenacher, M., Flemmig, T.F. (2006) Sampling strategy for intraoral detection of periodontal pathogens before and following periodontal therapy. Journal of Periodontology, 77(8): 1323
de Malheiros, V.J., Avila-Mario, J. (2004) Detection of pathogens from periodontal lesions. Rev Saude Publica, 38(5): 723-8
Đajić, D., Đukanović, D., Stanić, S., Kovačević, K. (2001) Bolesti usta, parodontologija, atlas. Beograd: Elit Medica
Feng, X.H., Zhang, L., Meng, H.X., i dr. (2006) Prevalence of putative periodontal microorganisms in Chinese patients with aggressive periodontitis. Zhonghua Kou Qiang YI Xue Za Zhi, (6); 41:344-7
Foschi, F., Cavrini, F., Montebugnoli, L., Stashenko, P., Sambri, V., Prati, C. (2005) Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol, 20(5): 289-95
Gomes, B.P.F.A., Pinheiro, E.T., Gade-Neto, C.R., Sousa, E.L.R., Ferraz, C.C.R., Zaia, A.A., Teixeira, F.B., Souza-Filho, F.J. (2004) Microbiological examination of infected dental root canals. Oral Microbiol Immunol, 19(2): 71-6
Jacinto, R.C., Gomes, B.P.F.A., Ferraz, C.C.R., Zaia, A.A., Filho, S.F.J. (2003) Microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and the antimicrobial susceptibility of some isolated anaerobic bacteria. Oral Microbiol Immunol, 18(5): 285-92
Jung, I.Y., Choi, B.K., Kum, K.Y., Roh, B.D., Lee, S.J., Lee, C.Y., Park, D.S. (2000) Molecular epidemiology and association of putative pathogens in root canal infection. J Endod, 26(10): 599-604
Mullally, B.H., Dace, B., Shelburne, C.E., Wolff, L.F., Coulter, W.A. (2000) Prevalence of periodontal pathogens in localized and generalized forms of early-onset periodontitis. J Periodontal Res, 35(4): 232-41
Ohnishi, M. (2006) Quantitative analysis of periodontal pathogens in aggressive periodontitis patients in Japanese population. Kokubyo Gakkai Zasshi, 73, str. 70-8
Okada, M., Hayashi, F., Nagasaka, N. (2001) PCR detection of 5 putative periodontal pathogens in dental plaque samples from children 2 to 12 years of age. J Clin Periodontol, 28(6): 576-82
Okuda, K., Ishihara, K., Nakagawa, T., Hirayama, A., Inayama, Y., Okuda, K. (2001) Detection of Treponema denticola in atherosclerotic lesions. J Clin Microbiol, 39(3): 1114-7
Peters, S.R., Valdez, M., Riviere, G., Thomas, D.D. (1999) Adherence to and penetration through endothelial cells by oral treponemes. Oral Microbiol Immunol, 14(6): 379-83
Rudney, J.D., Chen, R., Pan, Y. (2003) Endpoint quantitative PCR assays for Bacteroides forsythus, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans. J Periodontal Res, 38(5): 465-70
Samaranayake, L.P. (2002) Essential microbiology for dentistry. Edinburgh, itd: Churchill-Livingstone
Siqueira, J.F., Rôças, I.N. (2003) Bacteroides forsythus in primary endodontic infections as detected by nested PCR. J Endod, 29(6): 390-3
Takeuchi, Y., Umeda, M., Sakamoto, M., Benno, Y., Huang, Y., Ishikawa, I. (2001) Treponema socranskii, Treponema denticola, and Porphyromonas gingivalis are associated with severity of periodontal tissue destruction. J Periodontol, 72(10): 1354-63
Taylor, D., Aduse, J., Sayed, P., Slaney, J.M., Thomas, B.J., Curtis, M.A. (2002) Oro-dental bacteria in various atherosclerotic arteries. Eur J Clin Microbiol Infect Dis, 21(10): 755-7


article language: Serbian
document type: Paper
DOI: 10.2298/SARH0810476M
published in SCIndeks: 11/12/2008