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2011, vol. 50, iss. 4, pp. 35-39
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Interaction of biomaterials containing calcium hydroxyapatite/poly-L-lactide with the simulated body fluid
Interakcija biomaterijala na bazi kalcijum hidroksiapatita /poli-L-laktida i simulirane telesne tečnosti
aUniveristy of Niš, Faculty of Medicine, Institute of Biology and Human Genetics, Serbia bUniveristy of Niš, Faculty of Medicine, Department of Pharmacy, Serbia cUniveristy of Niš, Faculty of Medicine, Institute of Biomedical Research, Serbia dSerbian Academy of Sciences and Arts - SASA, Institute of Technical Sciences , Belgrade, Serbia eUniveristy of Niš, Faculty of Science, Department of Biology and Ecology, Serbia
email: marijavukelic@medfak.ni.ac.rs
Project: Virtual human osteoarticular system and its application in preclinical and clinical practice (MESTD - 41017)
Abstract
The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.
Sažetak
Svrha biomaterijala je da zamene deo ili funkciju dela tela na siguran, fiziološki i ekonomski prihvatljiv način. Rekonstrukcija koštanih defekata oduvek je predstavljala veliki problem ortopedije i maksilofacijalne hirurgije. Otkrićem hidroksiapatita (Hap) kao sastavnog i najzastupljenijeg dela kostiju sisara omogućen je razvoj fosfatnih keramika kao potencijalnog materijala za implantaciju. U ovom radu ispitivano je da li i na koji način biomaterijal kalcijum hidroksiapatit/poli-L-laktid (HAp/PLLA) interaguje sa jonskim sastavom ljudske plazme. Simulirana telesna tečnost (SBF) je veštački napravljena tečnost jonskog sastava i jonske koncentracije bliske ljudskoj plazmi. HAp/PLLA je inkubiran 1, 2, 3 i 5 nedelja u SBF-u. Površina tretiranog i netretiranog materijala ispitivana je skening elektronskom mikroskopijom (SEM) i EDS analizom (Energy dispersive X-ray spectroscopy), dok je SBF u kome je materijal inkubiran podvrgnut merenju električne provodljivosti i pH vrednosti. Dobijeni rezultati pokazuju da u SBF-u degradacija materijala HAp/PLLA kreće od površine materijala i da je uzrokovana rastvaranjem njegove polimerne komponente i precipitacije materijala sličnog hidroksiapatitu na njegovoj površini. Ovaj materijal pokazuje karakteristike koje ga svrstavaju u kandidate za primenu u ortopediji i maksilofacijalnoj hirurgiji.
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