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2012, vol. 27, br. 1, str. 59-67
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Skrining test u detekciji osetljivosti krompirove zlatice na insekticide
Screening test for detection of Leptinotarsa decemlineata (Say) sensitivity to insecticides
aUniverzitet u Novom Sadu, Poljoprivredni fakultet, Srbija
bUniverzitet u Kragujevcu, Agronomski fakultet, Čačak, Srbija
cMinistry of Agriculture, Trade, Forestry and Water Management, Belgrade
Projekat: Razvoj integrisanih sistema upravljanja štetnim organizmima u biljnoj proizvodnji sa ciljem prevazilaženja rezistentnosti i unapređenja kvaliteta i bezbednosti hrane (MPNTR - 46008)
Sažetak
Tokom 2009. godine ispitana je osetljivost 15 poljskih populacija krompirove zlatice (Leptinotarsa decemlineata Say.) - CPB na insekticide. Ispitivanje je izvedeno screening testom koji je omogućio brzu procenu osetljivosti prezimelih imaga na insekticide. Ocenjena je osetljivost na četiri insekticida (hlorpirifos, cipermetrin, tiametoksam i fipronil), koji se najčešće primenjuju u suzbijanju CPB u Srbiji. Insekticidi su primenjeni u preporučenoj, dva, pet i 10 puta većim količinama od preporučene, metodom potapanja (5 sec). Smrtnost insekata je određena posle 72h. Od 15 ispitanih populacija CPB, na količinu za praktičnu primenu hlorpirifosa samo su dve osetljive, jedna je blago rezistentna, 11 je rezistentno i jedna je visokorezistentna. Dve populacije su osetljive, dve blago rezistentne, pet je rezistentno i šest visokorezistentno na cipermetrin. Visokoosetljivo je 12 populacija na količinu za praktičnu primenu tiametoksama, a tri su osetljive. Na fipronil, dve su visokoosetljive populacije, šest je osetljivo, jedna blago rezistentna i šest je rezistentno. Primenom povećanih količina insekticida (2, 5 i 10 puta) od preporučenih, što opravdava samo metoda biotesta, došlo se brzo do saznanja o osetljivosti poljskih populacija CPB na insekticide.
Abstract
In 2009, the sensitivity of 15 field populations of Colorado potato beetle (Leptinotarsa decemlineata Say.) - CPB was assessed to chlorpyrifos, cypermethrin, thiamethoxam and fipronil, four insecticides which are mostly used for its control in Serbia. Screening test that allows rapid assessment of sensitivity of overwintered adults to insecticides was performed. Insecticides were applied at label rates, and two, five and 10 fold higher rates by soaking method (5 sec). Mortality was assessed after 72h. From 15 monitored populations of CPB, two were sensitive to label rate of chlorpyrifos, one was slightly resistant, 11 were resistant and one population was highly resistant. Concerning cypermethrin, two populations were sensitive, two slightly resistant, five were resistant and six highly resistant. Highly sensitive to thiamethoxam label rate were 12 populations, while three were sensitive. In the case of fipronil applied at label rate, two populations were highly sensitive, six sensitive, one slightly resistant and six were resistant. The application of insecticides at higher rates (2, 5 and 10 fold), that is justified only in bioassays, provided a rapid insight into sensitivity of field populations of CPB to insecticides.
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