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2013, vol. 11, br. 1, str. 45-53
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Antimikrobna aktivnost metanolnih ekstrakata četiri vrste lišajeva iz porodice Parmeliaceae
Antimicrobial activity of methanol extracts of four Parmeliaceae lichen species
aUniverzitet u Nišu, Medicinski fakultet, Katedra Farmacija bUniverzitet u Nišu, Prirodno-matematički fakultet, Odsek Hemija cUniverzitet u Nišu, Prirodno-matematički fakultet, Odsek Biologija i ekologija
e-adresa: igor.pfc@hotmail.com
Projekat: Dobijanje, fizičko-hemijska karakterizacija, analitika i biološka aktivnost farmakološki aktivnih supstanci (MPNTR - 172044)
Sažetak
U ovom radu je disk-difuzionom metodom (1 mg uzorka ekstrakta po disku; ekstrakti su rastvarani u metanolu; koncentracija je bila 25 mg/mL) određena antimikrobna aktivnost metanolnih ekstrakata četiri vrste lišaja iz porodice Parmeliaceae (Hypogymnia physodes (L.) Nyl., Evernia prunastri (L.) Ach., Flavoparmelia caperata (L.) Hale i Parmelia sulcata Taylor). Testirana je aktivnost na 11 sojeva Gram-pozitivnih (Enterococus sp., Bacillus subtilllis, Sarcina lutea, Micrococus luteus, Staphylococcus aureus, Clostridium sporogenes) i Gram-negativnih bakterija (Escherichia coli, Proteus vulgaris, Salmonela enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae), i po jedna plesan (A. niger) i kvasac (C. albicans). Svi testirani ekstrakti su pokazali umerenu antimikrobnu aktivnost. Multivarijantna statistička analiza (aglomerativna hijerarhijska klaster analiza) dobijenih rezultata omogućila je da se testirani uzorci grupišu na osnovu sličnosti/razlika u pokazanoj antimikrobnoj aktivnosti prema različitim sojevima mikroorganizama: antimikrobni profili ekstrakata H. physodes i E. prunastri su bili uporedivi; slično važi za ekstrakte F. caperata i P. sulcata. Pored toga, na osnovu sličnosti/razlika u osetljivosti na dejstvo ekstrakata proučavanih lišajeva, ovde testirani mikroorganizmi se mogu podeliti u dve grupe: Grupa I - P. vulgaris, K. pneumoniae (Gram-negativne bakterije), A. niger i C. albicans; Grupa II - E. coli, S. enteritidis, P. aeruginosa (Gram-negativne bakterije) i sve ispitivane Gram-pozitivne vrste.
Abstract
Antimicrobial activity of methanol extracts of four Parmeliaceae lichens (Hypogymnia physodes (L.) Nyl., Evernia prunastri (L.) Ach., Flavoparmelia caperata (L.) Hale and Parmelia sulcata Taylor) against a panel of microbial strains (11 Gram-positive (Enterococus sp., Bacillus subtilllis, Sarcina lutea, Micrococus luteus, Staphylococcus aureus, Clostridium sporogenes) and Gram-negative bacteria (Escherichia coli, Proteus vulgaris, Salmonela enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae), the filamentous fungus A. niger and the yeast C. albicans) was assayed using a disk diffusion method (1 mg of the extract per disc; extracts were dissolved in methanol, 25 mg/mL). All tested extracts showed moderate antimicrobial activity. Multivariate statistical treatment (agglomerative hierarchical clustering analysis, AHC) of the obtained results allowed grouping of the samples according to their antimicrobial potential against different strains: antimicrobial profile of H. physodes and E. prunastri extracts were comparable; the similar is true for F. caperata and P. sulcata samples. In addition, based on the similarities/ dissimilarities in their susceptibility toward the tested extracts, two groups of microorganisms could be distinguished: Group I - P. vulgaris, K. pneumoniae (Gram-negative bacteria), A. niger and C. albicans; Group II - E. coli, S. enteritidis, P. aeruginosa (Gram-negative bacteria) and all of the assayed Gram-positive strains.
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