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2018, vol. 75, br. 5, str. 473-480
Mezenhimske matične ćelije iz periapeksnih lezija moduliraju produkciju citokina od strane lokalnih imunskih ćelija
aVojnomedicinska akademija, Institut za medicinska istraživanja, Beograd + Univerzitet u Nišu, Medicinski fakultet
bUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd
cVojnomedicinska akademija, Institut za medicinska istraživanja, Beograd
dVojnomedicinska akademija, Institut za medicinska istraživanja, Beograd + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd
eUniverzitet u Beogradu, Stomatološki fakultet, Klinika za oralnu hirurgiju
fUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd + Univerzitet u Beogradu, Institut za primenu nuklearne energije - INEP

e-adresamjcolic@eunet.rs
Projekat:
Primena funkcionalizovanih ugljeničnih nanocevi i nanočestica zlata za pripremu dendritskih ćelija u terapiji tumora (MPNTR - 175102)
Project by the Faculty of Medicine of the Military Medical Academy, University of Defence in Belgrade, No: MF-VMA/10/16-18

Sažetak
Pokazano je da mezenhimske matične ćelije (engl. mesenchymal stem cells - MSC) suprimiraju imunske i inflamacijske reakcije. Međutim, nije poznato da li MSC iz inflamacijom zahvaćenih tkiva, kao što su periapeksne lezije (engl. periapical lesions - PLs) ispoljavaju slične efekte. Upravo je ovo pitanje razmatrano u našem radu čiji cilj je bio da se ispita sposobnost PL-MSC da moduliraju produkciju citokina od strane imunskih ćelija lokalno. Metode. MSC su izolovane iz asimptomatskih (engl. asymptomatic - as) i simptomatskih (engl. symptomatic - sy) PL zuba. Njihov fenotip je određen metodom protočne citofluorimetrije na osnovu detekcije površinskih markera specifičnih za MSC. Antiinflamacijska i imunomodulacijska svojstva PLMSC ispitivana su merenjem produkcije citokina u kokulturi sa mononuklearnim ćelijama (engl. mononuclear cells - MNCs) izolovanim iz asPLs i syPLs. Nivo produkovanih citokina u supernatantima određivan je ELISA metodom. Rezultati. Obe PL-MSC linije karakterisao je fenotip tipičan za druge tipove MSC u kome je dominirala ekspresija CD29, CD44, CD90, CD105 i CD166 markera. Izolovane PL-MSC linije su, nezavisno od fenotipske sličnosti, ispoljile isti modulacijski efekat na produkciju citokina, ali je odgovor asPL-MNCs i syPL-MNCs bio različit, uprkos sličnom sastavu MNCs u oba tipa lezija. Oba tipa MSC linija inhibirala su produkciju proinflamacijskih citokina kao što su interleukin 1 (IL-1) i faktor nekroze tumora (TNF-). Međutim, produkcija IL-8 bila je snižena jedino u kokulturi sa syPL-MNCs. PL-MSC linije su takođe modulirale produkciju imunoregulacijskih citokina. Produkcija transformišućeg faktora rasta (TGF-) bila je povećana u kokulturama sa oba tipa MNC, i asPL-MNCs i syPLMNCs, a nivo IL-10 bio je povećan jedino u kokulturi sa asPL-MNCs. Zaključak. Naši rezultati pokazuju da PLMSC doprinose smanjenju lokalne inflamacije i imunskog odgovora, ali je ovaj efekat verovatno manje efikasan u toku egzacerbacije inflamacije u PLs.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/VSP160901272M
objavljen u SCIndeksu: 09.08.2018.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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