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2009, vol. 60, br. 4, str. 247-256
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Etiološka proučavanja bakteriozne vlažne truleži uskladištenih glavica komorača
Etiology of soft rot on fenel bulbs
Institut za zaštitu bilja i životnu sredinu, Beograd, Srbija
Projekat: Projekat Ministarstva nauke Republike Srbije, br. 20051
Sažetak
U radu su proučene patogene i biohemijske odlike fitopatogenih bakterija izolovanih iz glavica komorača sa simptomima truleži. Proučavani sojevi prouzrokuju trulež glavica komorača, veštački inokulisanih, plodova paprike, kao i kriški mrkve i krompira i pri tome ispoljavaju izraženu pektolitičku aktivnost. Proučavanjem bakterioloških odlika utvrđeno je da su izolovani sojevi Gramnegativni, ne fluoresciraju na Kingovoj podlozi B, glukozu metabolišu i u aerobnim i anaerobnim uslovima, ne stvaraju levan i oksidazu. Na osnovu navedenih karakteristika zaključeno je da ispitivani sojevi pripadaju rodu Pectobacterium. Proučavani sojevi ne stvaraju fosfatazu, lecitinazu, indol, redukujuće supstance iz saharoze, a na Loganovoj podlozi formiraju karakteristične kolonije ružičaste boje sa tamnim centrom. Na osnovu ovih, ali i rezultata ostalih diferencijalnih testova za vrste roda Pectobacterium (metabolizam ugljenih hidrata, razvoj pri 5% NaCl, osetljivost prema eritromicinu), zaključeno je da vlažnu trulež komorača prouzrokuje bakterija Pectobacterium carotovorum subsp. carotovorum. Ovo je prvo saopštenje o nalazu ove bakterije kao patogena komorača u Srbiji.
Abstract
Soft rot bacterial strains were isolated from diseased fennel bulbs. They were characterised on the basis of its pathogenicity morphological, cultural and biochemical properties. All investigated strains caused the soft rot of inoculated fenel bulbs, pepper fruits as well as clices of potato and carrot. Results of bacteriological properties show that soft rot of fennel bulbs were caused by Gram negative, nonfluorescent, facultative anaerobic, levan and oxidase negative bacteria belonging to the genus Pectobacterium. The investigared strains utilised lactose, grew at 5% NaCl and weak growth was recorded on 37oC; they are resistent to erytromicin. Negative results were recorded in indol, phospathase, lecithinase and reducing compounds pf sucrose tests. These results as well as the characteristics growth on Logan differential medium indicated that sof rot of fenel bulbs was caused by Pectobacterium carotovorum subsp. carotovorum. This is the first report of this bacterium affecting fennel bulbs in Serbia.
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Reference
|
|
12  
|
Arsenijević, M. (1992) Fitopatogene bakterije. Beograd: Naučna knjiga
|
|
63
|
Arsenijević, M. (1997) Bakterioze biljaka. Novi Sad: S print
|
|
|
Arsenijević, M., Đurišić, S., Milošević, D. (1994) Erwinia carotovora subsp. atroseptica, potato pathogen in our country. Zaštita bilja, 45(3): 169-178
|
|
1
|
Arsenijević, M., Trkulja, V., Mitrović, P. (1996) Bakteriozna trulež glavica kupusa. Zaštita bilja, 474(218): 301-311
|
|
3  
|
Arsenijević, M., Obradović, A. (1996) Occurrence of bacterial wilt and soft rot of seed cabbage plants (Brassica oleracea var capitata L) in Yugoslavia. Journal of phytopathology, 144(6): 315-319
|
|
1
|
Arsenijević, M., Trkulja, V., Obradović, A. (1997) Pathogenic and bacteriological characteristics of Yugoslav Erwinia soft rot strains originating from pepper and eggplant fruits. Zeitschrift fur pflanzenkrankheiten und pflanzenschutz, 104(4): 394-402
|
|
3
|
Bradburry, J.F. (1986) Guide to plant pathogenic bacteria. CAB International Mycological Institute
|
|
|
Cother, E.J., Sivasithamparam, K. (1983) Erwinia the carotovora group. u: Fahy, P.C.and Persley, G.J. [ur.] Plant bacterial diseases: A dignostic guide, Sydnez , Australia: Academic Press
|
|
1
|
de Boer, S.H., Kelman, A. (2001) Erwinia soft rot. u: Schaad, N.W., Jones J.B., Chun, W. [ur.] Laboratory guide for Identification of plant Pathogenic bacteria, St. Paul, Minnesota: APS Press, Third Edition.pp. 56-73
|
|
|
Degefu, Y., Virtanen, E., Vairinen, T. (2008) Pre-PCR processes in the molecular detection of blackleg and soft rot Erwiniae in seed potatoes. J. Phytopathology, 157: 370- 378
|
|
|
Đinović, I. (2002) Svet povrća. Beograd: Reprograf
|
|
|
Farrar, J.J. (2002) Soft rot. u: Davis, M.R., Raid R.N [ur.] Compendium of umbelliferous crop diseases, St Paul, Minnesota: APS, pp.14-15
|
|
|
Gardan, L., Gouy, C., Christen, R., Samson, R. (2003) Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. Int J Syst Evol Microbiol, 53(Pt 2): 381-91
|
|
4
|
Gavrilovic, V.M., Obradovic, A., Arsenijevic, M. (2001) Bacterial soft rot of carrot, parsley and celery. Plant Pathogenic Bacteria, str. 269-271
|
|
|
Gavrilović, V., Milijašević, S., Arsenijević, M. (2007) Pseudomonas marginalis - prouzrokovač truleži korena celera i paštrnaka. Zaštita bilja, vol. 58, br. 1-4, str. 5-13
|
|
1
|
Ivanović, M., Gašić, K., Gavrilović, V., Obradović, A. (2009) Pectobacterium carotovorum subsp. Carotovorum - prouzrokovač vlažne truleži biljaka kale u Srbiji i Crnoj Gori. Pesticidi i fitomedicina, vol. 24, br. 4, str. 287-293
|
|
|
Janse, J.D., Spit, B.E. (1989) A note on limutations of identifying soft rot Erwinias by temperature tolerances and sensitivity to eritromycin on a pectate medium. Journal of Phytopathology, 125(3): 265
|
|
|
Jovanović, O. (1998) Patogene karakteristike grupe Erwinia carotovora različitog porekla. Zaštita bilja, Vol. 49 (1), No 223: 29-55
|
|
1
|
Jovanović, O. (1998) Biohemijsko-fiziološke karakteristike bakterija grupe 'Erwinia carotovora' različitog porekla. Zaštita bilja, 49(2): 169-180
|
|
10
|
Klement, Z. (1990) Inoculation plant tissues: Cancer and dieback disease. u: Klement, Z., Rudolph, K., Sands, D. [ur.] Methods in Phytobacteriology, Budapest: Akademiai Kiado, str. 105-106
|
|
|
Laurila, J., Hannukkala, A., Nykyri, J., Pasanen, M., Helias, V., Garlant, L., Pirhonen, M. (2009) Symptoms and yield reduction caused by Dickeya spp. Strains isolated from potato and river water in Finland. European Journal of Plant Pathology, 126, str. 249-262
|
|
26
|
Lelliott, R.A., Stead, D.E. (1987) Methods for the diagnosis of bacterial diseases of plants. Oxford, UK: Blackwell Scientific Publications
|
|
|
Nuh, B., Bastas, K.K., Maden, S., Yasar, A. (2006) Bacterial leaf and peduncle soft rot caused by Pectobacterium carotovorum on tulips in Konya, Turkey. Phytoparasitica, 34 (3), str. 279-280
|
|
3
|
Obradović, A. (1996) Patogene i biohemijsko-fiziološke karakteristike bakterija grupe Erwinia carotovora parazita krompira. Zaštita bilja, 215, str. 57-70
|
|
4
|
Obradović, A. (1999) Identifikacija bakterija rodova Pseudomonas i Erwinia patogena salate i karfiola. Zaštita bilja, 50, 9-60
|
|
|
Samson, R., Legendre, J.B., Christen, R., Fischer-Le, S.M., Achouak, W., Gardan, L. (2005) Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species,. Int J Syst Evol Microbiol, 55(Pt 4): 1415-27
|
|
|
|
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