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2019, vol. 35, iss. 1, pp. 61-69
Mycobiota and aflatoxin B1 in poultry feeds
aInstitute for Animal Husbandry, Belgrade-Zemun, Serbia
bUniversity of Belgrade, Faculty of Agriculture, Serbia
cMaize Research Institute 'Zemun Polje', Belgrade-Zemun, Serbia
Project:
Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods (MESTD - 31023)
Sustainable conventional and revitalized traditional production of poultry meat and eggs with added value (MESTD - 31033)
Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (MESTD - 46010)

Abstract
In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
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article language: English
document type: Original Scientific Paper
DOI: 10.2298/BAH1901061K
published in SCIndeks: 16/07/2019
Creative Commons License 4.0

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