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2014, vol. 30, iss. 4, pp. 731-741
Natural mycobiota and aflatoxin B1 presence in bee pollen collected in Serbia
aUniversity of Belgrade, Faculty of Agriculture, Serbia
bVeterinary Specialist Institute, Kraljevo
cInstitute for Animal Husbandry, Belgrade-Zemun, Serbia
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (MESTD - 46008)
Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (MESTD - 46009)
Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (MESTD - 46010)

Keywords: bee pollen; fungi; aflatoxin B1
Total fungal count, incidence of fungi and aflatoxin B1 (AFB1) concentration were studied in 33 samples of bee pollen randomly collected from beekeepers in Serbia. The total number of fungi was determined by dilution method whereas AFB1 was detected using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The mycological estimation showed the presence of nine genera of fungi as followed: Acremonium, Alternaría, Aspergillus, Cladosporium, Epiccocum, Fusarium, Mucor, Pénicillium and Rhizopus, with total number ranging from 1 x 103 to 1 x 105 CFU g-1. The results have shown the predominance of the fungi from the genera Aspergillus and Alternaria. Among Aspergillus species it was observed that the most frequent species was A. flavus with incidence of 27.27 %. Mycotoxin AFB1 was detected as 100% positive in all samples (100%) with an average concentration of 8.61 μg kg-1. The obtained results indicated that honey bee pollen must be strictly controlled during its manipulation in the harvesting and manufacturing. Therefore, the implementation of good manufacturing (beekeeping) practice to define procedures for honeybee products could be crucial to reduce the risk of possible contamination and provide natural and safety product without risk on the human health.
*** (2013) Regulation for the quality and other requirements for the honey and other honeybee products and for the preparation based on honey or honeybee products. Official gazette
American Society Of Analytical Chemists, (AOAC) (1997) Official method 925.09. in: Official methods of analysisth, Arlington, VA: AOAC, 1997, 16th ed
Bogdanov, S. (2012) Pollen: Production, nutrition and health: A review. Bee product science,
Bonvehí, J. S., Jordà, R. E. (1997) Nutrient Composition and Microbiological Quality of Honeybee-Collected Pollen in Spain. Journal of Agricultural and Food Chemistry, 45(3): 725-732
Brindza, J., Gróf, J., Bacigálová, K., Ferianc, P., Tóth, D. (2010) Pollen microbial collonization and safety. Acta Chimica Slovaca, 95-102, 3, 1
Bruns, H. A. (2003) Controlling Aflatoxin and Fumonisin in Maize by Crop Management. Toxin Reviews, 22(2-3): 153-173
Carpes, S.T. (2009) Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil. Brazilian journal of food technology, 12(03): 220-229
de Melo, I.L.P., de Almeida-Muradian, L.B. (2011) Comparison of methodologies for moisture determination on dried bee pollen samples. Ciência e Tecnologia de Alimentos, 31(1): 194-197
González, G., Hinojo, M.J., Mateo, R., Medina, A., Jiménez, M. (2005) Occurrence of mycotoxin producing fungi in bee pollen. International Journal of Food Microbiology, 105(1): 1-9
Groopman, J.D., Cain, L.G., Kensler, T.W., Harris, C.C. (1988) Aflatoxin Exposure in Human Populations: Measurements and Relationship to Cancer. Critical Reviews in Toxicology, 19(2): 113-145
Hani, B., Dalila, B., Saliha, D., Harzallah, D., Ghadbane, M., Khennouf, S. (2012) Microbiological sanitary aspects of pollen. Advances in environmental biology. 6, 1415-1420
Harley, R.M. (1997) Mycotoxins in cereals. in: D'Mello J.F.D. [ed.] Mycotoxins and Environmental Health. Handbook for SAC Mycotoxin Workshop, Edinburgh, September
Kačániová, M., Juráček, M., Chlebo, R., Kňazovická, V., Kadasi-Horáková, M., Kunová, S., Lejková, J., Haščík, P., Mareček, J., Šimko, M. (2011) Mycobiota and mycotoxins in bee pollen collected from different areas of Slovakia. Journal of Environmental Science and Health, Part B, 46(7): 623-629
Kačánjuvá, M., Fikselová, M. (2007) Mycological flora on tree fruits, crusts, leaves and pollen Sorbus domestica L. Annals of Agriculturals and Environmental Medicine, 229-232, 14
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Stojanović, Lj., Radović, Č., Gogić, M. (2012) Distribution of moulds and mycotoxins in maize grain silage in the trench silo. Biotechnology in Animal Husbandry, vol. 28, br. 4, str. 845-854
Krnjaja, V.S., Lević, J.T., Stanković, S.Ž., Petrović, T.S., Lukić, M.D. (2013) Molds and mycotoxins in freshly harvested maize. Zbornik Matice srpske za prirodne nauke, vol. , br. 124, str. 111-119
Lacey, J., Magan, N. (1991) Fungi colonising cereal grain: Their occurrence and water and temperature relationships. in: Chelkowski J. [ed.] Cereal grain: Mycotoxins, fungi and quality in drying and storage, Amsterdam: Elsevier Science, pp. 77-118
Lazarevic, K.B., Andric, F.L., Trifkovic, J.D., Tesic, Z.L., Milojkovic-Opsenica, D.M. (2012) Characterisation of Serbian unifloral honeys according to their physicochemical parameters. Food Chemistry, vol. 132, br. 4, str. 2060-2064
Leja, M., Mareczek, A., Wyżgolik, G., Klepacz-Baniak, J., Czekońska, K. (2007) Antioxidative properties of bee pollen in selected plant species. Food Chemistry, 100(1): 237-240
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A., Stepanić, A. (2013) An outbreak of Aspergillus species in response to environmental conditions in Serbia. Pesticidi i fitomedicina, vol. 28, br. 3, str. 167-179
Llnskens, H.F., Jorde, W. (1997) Pollen as food and medicine-A review. Economic Botany, 51(1): 78-86
Logrieco, A., Bottalico, A., Mule, G., Moretti, A., Perrone, G. (2003) Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. European Journal of Plant Pathology, 109, str. 645-667
Magan, N., Olsen, M. (2004) Mycotoxins in food: Detection and control. Abington Hall: CRC press, Abington, MA, USA
Medina, A., Gonzáles, G., Sáez, J.M., Mateo, R., Jiménez, M. (2004) Bee pollen, a substrate that stimulates ochratoxin a production by wilh. Systematic and Applied Microbiology, 27(2): 261
Mutsaers, M., Blitterswijk, H., Leven, L., Kerkvliet, J., Waerdt, J. (2005) Bee products. Properties, processing and marketing. Wageningen: Agromisa Foundation, Netherlands
Nedić, N., Jevtić, G., Jež, G., Anđelković, B., Milosavljević, S., Kostić, M. (2011) Diferencijacija prednjeg krila medonosne pčele iz Srbije. Biotechnology in Animal Husbandry, vol. 27, br. 3, str. 1387-1394
Nedić, N., Mladenović, M., Dugalić-Vrndić, N., Milosavljević, S. (2008) Dobra pčelarska praksa uslov kvalitetnije proizvodnje meda, Xvi naučno savetovanje sa međunarodnim učešćem 'kvalitetom i selekcijom u pčelarstvu ka Evropi'. Beograd: Poljoprivredni fakultet, (pp. 121-128), 9-10 februar
Nogueira, C., Iglesias, A., Feás, X., Estevinho, L.M. (2012) Commercial Bee Pollen with Different Geographical Origins: A Comprehensive Approach. International Journal of Molecular Sciences, 13(12): 11173-11187
Pitta, M., Markaki, P. (2010) Study of aflatoxin B1 production by Aspergillus parasiticus in bee pollen of Greek origin. Mycotoxin Research, 26(4): 229-234
Rustom, I.Y.S. (1997) Aflatoxin in food and feed: Occurrence, legislation and inactivation by physical methods. Food Chemistry, 59(1): 57
Snowdon, J.A., Cliver, D.O. (1996) Microorganisms in honey. International journal of food microbiology, 31(1-3): 1-26
Tarr, B. (2006) Managing the effects of molds and mycotoxins in ruminants. Shur-gain, nutreco Canada inc
Watanabe, T. (1994) Pictorial atlas of soil and seed fungi morphologies of cultured fungi and key to species. London-New York-Washington: Lewis Publishers


article language: English
document type: Original Scientific Paper
DOI: 10.2298/BAH1404731P
published in SCIndeks: 15/05/2015

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